Open Access

Mechanisms of microRNA‑142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy

  • Authors:
    • Du Xiao
    • Jingdan Lv
    • Zhigang Zheng
    • Yi Liu
    • Yonggen Zhang
    • Cuizhu Luo
    • Liu Qi
    • Bing Qin
    • Chao Liu
  • View Affiliations

  • Published online on: April 8, 2021     https://doi.org/10.3892/ijmm.2021.4931
  • Article Number: 98
  • Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Researchers have confirmed the microRNA (miRNA/miR)‑epilepsy association in rodent models of human epilepsy via a comprehensive database. However, the mechanisms of miR‑142 in epilepsy have not been extensively studied. In the present study, a rat model of epilepsy was first established by an injection of lithium chloride‑pilocarpine and the successful establishment of the model was verified via electroencephalogram monitoring. The levels of miR‑142, phosphatase and tensin homolog deleted on chromosome 10 (PTEN)‑induced putative kinase 1 (PINK1), marker proteins of mitochondrial autophagy, and apoptosis‑related proteins were measured. Additionally, the pathological changes in the hippocampus, the ultrastructure of the mitochondria, and degeneration and the apoptosis of neurons were observed using different staining methods. The malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in the hippocampus, mitochondrial membrane potential (MTP) and reactive oxygen species (ROS) generation were detected. Furthermore, the targeting association between miR‑142 and PINK1 was predicted and verified. Consequently, apoptosis increased, and mitochondrial autophagy decreased, in the hippocampus of epileptic rats. Following miR‑142 inhibition, the epileptic rats exhibited an increased Bax expression, a decreased Bcl‑2 expression, upregulated marker protein levels of mitochondrial autophagy, a reduced MDA content, an enhanced SOD activity, an increased MTP and decreased ROS generation. PINK1 is a target gene of miR‑142, and its overexpression protected against hippocampal damage. Taken together, the results of the present study demonstrated that miR‑142 inhibition promotes mitochondrial autophagy and reduces hippocampal damage in epileptic rats by targeting PINK1. These findings may provide useful information for the treatment of epilepsy.
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June-2021
Volume 47 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Xiao D, Lv J, Zheng Z, Liu Y, Zhang Y, Luo C, Qi L, Qin B and Liu C: Mechanisms of microRNA‑142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy. Int J Mol Med 47: 98, 2021
APA
Xiao, D., Lv, J., Zheng, Z., Liu, Y., Zhang, Y., Luo, C. ... Liu, C. (2021). Mechanisms of microRNA‑142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy. International Journal of Molecular Medicine, 47, 98. https://doi.org/10.3892/ijmm.2021.4931
MLA
Xiao, D., Lv, J., Zheng, Z., Liu, Y., Zhang, Y., Luo, C., Qi, L., Qin, B., Liu, C."Mechanisms of microRNA‑142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy". International Journal of Molecular Medicine 47.6 (2021): 98.
Chicago
Xiao, D., Lv, J., Zheng, Z., Liu, Y., Zhang, Y., Luo, C., Qi, L., Qin, B., Liu, C."Mechanisms of microRNA‑142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy". International Journal of Molecular Medicine 47, no. 6 (2021): 98. https://doi.org/10.3892/ijmm.2021.4931