Open Access

KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3

Retraction in: /10.3892/ijmm.2023.5265

  • Authors:
    • Pengyi Zheng
    • Kaijie Wu
    • Zhongwei Gao
    • Huibing Li
    • Wensheng Li
    • Xiaohui Wang
    • Zhenguo Shi
    • Fei Xiao
    • Kaixuan Wang
    • Zhijun Li
    • Qingjiang Han
  • View Affiliations

  • Published online on: April 9, 2021     https://doi.org/10.3892/ijmm.2021.4932
  • Article Number: 99
  • Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bladder cancer (BC) is among the most common urinary system tumors with a high morbidity and mortality worldwide. Despite advancements being made in the diagnosis and treatment of bladder cancer, targeted therapy remains the most promising treatment, and novel therapeutic targets are urgently required in to improve the outcomes of patients with BC. Kinesin family member 4A (KIF4A) is a plus‑end directed motor protein involved in the regulation of multiple cellular processes, such as mitosis and axon growth. Notably, KIF4A plays important roles in tumor growth and progression, and its expression is associated with the prognosis of several types of cancer. However, the potential role and molecular mechanisms of KIF4A in bladder cancer development remain unclear. The present study demonstrated that KIF4A was highly expressed in human BC tissues, and its expression was associated with patient clinicopathological characteristics, such as tumor stage (P=0.012) and with the prognosis of patients with BC. It was further found that KIF4A promoted the cell proliferation of bladder cancer both in vitro and in vivo. On the whole, the data presented herein provide evidence that KIF4A promotes the development of BC through the transcriptional activation of the expression of CDCA3. The present study indicates the involvement of KIF4A in the progression of BC and suggests that KIF4A may be a promising therapeutic target for the treatment of BC.

Introduction

Bladder cancer (BC) is one of the most common type of urinary system tumors with a high morbidity and mortality worldwide (1-3). In 2017, approximately 80,000 new cases of BC were diagnosed in the USA, leading to almost 15,000 deaths (4,5). The traditional treatment methods for BC, such as surgical treatment, radiotherapy and chemotherapy, are largely dependent on the stage and grade of BC (6,7). The 5-year total survival rates for advanced BC remain <20% (8). Despite advancements being made in the diagnosis and treatment for BC, the survival rates of patients remained unaltered until the emergence of targeted therapy (9). The BC molecular targets, such as phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), epidermal growth factor receptor (EGFR) and neuroblastoma RAS viral oncogene homolog (NRAS), have already been studied, and therapeutic drugs targeting these proteins have been preliminarily developed (10). However, in order to improve the survival rates of patients with BC, novel and promising therapeutic targets are urgently required.

Kinesin family proteins are a class of microtubule-dependent motor proteins that travel along microtubule tracks and mediate multiple cellular processes, such as mitosis and vesicles transport (11-13). Kinesin family member 4A (KIF4A), a member of the kinesin family, is a plus end directed motor protein (14). Previous studies have indicated that KIF4A is dominantly localized in the nucleus and affects multiple cellular processes (15,16). KIF4A regulates mitosis through various mechanisms, including the regulation of spindle assembly, chromosome regulation and segregation (17,18). Additionally, KIF4A participates in the process of DNA repair and replication, and further maintains genetic stability (19). KIF4A also promotes axon growth in neuronal cells (14).

Importantly, KIF4A is highly expressed in multiple human tissues and is overexpressed in several types of tumors, such as colorectal cancer (20). Researchers have revealed the involvement of KIF4A in the growth and progression of multiple types of cancer (20-22). KIF4A could serve as a potential contributor of several types of cancer, including lung cancer, colorectal cancer, and hepatocellular carcinoma (HCC), and its expression has been shown to be associated with the prognosis of these types of cancer (20-22). Although the function of KIF4A in tumor development have gained increasing attention, the possible role and mechanisms of KIF4A in the progression of BC remain unclear.

Cell division cycle-associated protein 3 (CDCA3) is a cell cycle regulator involved in the progression of multiple types of cancer, such as breast cancer and gastric cancer (23,24). CDCA3 has been shown to affect the proliferation, migration and apoptosis of cancer cells, and could therefore serve as a promising therapeutic target for tumors (25). Several proteins promote the progression of cancers via CDCA3 (26).

The present study demonstrated that KIF4A was highly expressed in human BC tissues. The data further confirmed that KIF4A expression was associated with tumor stage and the prognosis of patients with BC. KIF4A promoted BC cell proliferation in vivo and in vitro. Importantly, it was confirmed that KIF4A promoted BC progression via transactivating the expression of CDCA3. Collectively, the findings of the present study indicate the involvement of KIF4A in the progression of BC and demonstrate that KIF4A may serve as a promising therapeutic target for the treatment of BC.

Materials and methods

Biological analysis

Biological analysis was conducted to investigate the mRNA levels of KIF4A in tumor and normal tissues and explore the link between KIF4A and the prognosis of patients with BC. Gene Expression Profiling Interactive Analysis (GEPIA; http://gepia.cancerpku.cn/detail.php?gene=KIF4A/) was used to collate and analyze The Cancer Genome Atlas (TCGA) data with a threshold of P<0.05 and LogFC >1 or <-1 for differential genes, and the median was used as the basis for dividing the patients into two groups for Kaplan-Meier survival analysis; the log rank test was used to assess significant differences. The 95% confidence interval is marked with a dotted line in the cell survival plots. All the data on the survival rates were obtained from TCGA.

Antibodies, primers and plasmids

Anti-KIF4A antibody [1:400 dilution for immunohistochemistry (IHC), 1:2,000 dilution for western blot analysis and 1:50 dilution for ChIP assay, ab122227; Abcam], anti-CDCA3 antibody (1:100 dilution, AB_2719030; Thermo Fisher Scientific, Inc.), anti-β-actin antibody (1:1,000 dilution, ab8226; Abcam), anti-Ki67 antibody (1:1,000 dilution, ab16667; Abcam), anti-proliferating cell nuclear antigen (PCNA) antibody (1:500 dilution, ab29; Abcam), anti-cyclin D1 antibody (1:500 dilution, ab16663; Abcam), anti-cyclin A antibody (1:500 dilution, ab185619; Abcam).

The sequences of primers used for reverse transcription-quantitative PCR (RT-qPCR) were as follows: KIF4A forward, 5′-TCTGTTTCAGGCTGCTTTCA-3′ and reverse, 5′-GCCCTGAAATATTTGATTGGAG-3′; CDCA3 forward, 5′-TGGTATTGCACGGACACCTA-3′ and reverse, 5′-TGTTTCACCAGTGGGCTTG-3′; and GAPDH forward, 5′-TGACTTCAACAGCGACACCCA-3′ and reverse, 5′-CACCCTGTTGCTGTAGCCAAA-3′.

The shRNA plasmids of KIF4A and CDCA3, as well as other plasmids, including pEnter-KIF4A, pEnter-CDCA3, and pGL-CDCA3 plasmids were constructed by the authors.

The shRNA sequences which specifically targeted KIF4A were as follows: 5′-AACAGGAAGAAGTCTTCAATACA-3′. In addition, the shRNA targeted sequences which specifically targeted CDCA3 were as follows: 5′-AACTGGAGGGTCTTAAACATGCC-3′.

Human tissue samples

A total of 159 human BC tissues and corresponding normal tissues were obtained from the First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology from March, 2020 to October, 2020. The relevant experiments were in line with the requirements of the Declaration of Helsinki and had been approved by the hospital. All patients were treated with surgery only, and no chemoradiotherapy was used. All patients were enrolled with informed consent. All studies were approved by the IACC of the First Hospital of Xi'an Jiaotong University (approval no. 2020-03-B003).

Through IHC staining, it was found that KIF4A was located in the nucleus of BC tissues. The intensity of staining was scored for 4 grades as follows: 0, negative staining; 1, low staining intensity; 2, medium staining intensity; 3, high staining intensity. The product (combination of proportion scores of positively stained cells and respective intensity scores) was used as the final staining score (a minimum value of 0 and a maximum value of 300). Scores of 0-100 were considered as low expression, and scores of 101-300 was considered as high expression.

CDCA3 was found to be expressed in the cytoplasm of BC samples. The expression level of CDCA3 was manually divided into 4 groups based on the staining intensity (0, negative staining; 1, low staining intensity; 2, medium staining intensity; 3, high staining intensity). Moreover, the proportion of stained cells was shown as follows (0, 0% stained cells; 1, 1-30% stained cells; 2, 31-60% stained cells; and 3, 61-100% stained cells) The score of staining intensity x the score of stained cells percentage <1 or =1 was considered as negative staining, a score of 2-4 was considered as low staining and a score >4 was considered as CDCA3 high staining.

The sections from each patient were observed within a total of 5 visual fields, and 2 experienced pathologists examined the sections.

Cells, cell culture and transfection

The T24 (SCSP-536) and 5637 (TCHu 1) human BC cells were purchased from the The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences and maintained in McCoy's 5a medium and RPMI-1640 culture medium (Gibco; Thermo Fisher Scientific, Inc.), respectively, supplemented with 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and incubated at 37°C in a 5% CO2 incubator.

The indicated plasmids in the present study were transfected into BC cells using Lipofectamine® 3000 (L3000015; Invitrogen; Thermo Fisher Scientific, Inc.). To perform transfection in 6-well plates, 1 μg plasmids and 5 μl Lipofectamine® were respectively added into 500 μl Opti-MEM (Gibco; Thermo Fisher Scientific, Inc.) for 5 min, subsequently mixed for 20 min and added to the cells without serum, after 4 h, the medium was refreshed with complete medium. The subsequent in vitro assays were performed after 24 h. The stable knockdown of KIF4A in the cell lines was achieved by the use of KIF4A depletion or control and used for the animal assays.

RT-qPCR

Total RNA was extracted from the T24 and 5637 cells using TRIzol® reagent (15596-018; Invitrogen; Thermo Fisher Scientific, Inc.). Total RNA was then reverse transcribed using the M-MLV reverse transcriptase kit (M1701; Promega Corporation). The primer sequences have been described above. qPCR was performed through the use of SYBR-Green mixture (RR420A; Takara Bio, Inc.), and the expression levels of KIF4A and CDCA3 were normalized to those of GAPDH. The thermocycling conditions were used for qPCR were as follows: Initial denaturation at 95°C for 3 min; followed by 30 cycles of denaturation at 95°C for 30 sec, annealing at 58°C for 30 sec and extension a 72°C for 30 sec. The 2−ΔΔCq method was used to quantify the results (27).

Western blot analysis

All the cell and tissue samples were lysed with RIPA buffer (R0278; Sigma-Aldrich; Merck KGaA) to isolate total proteins and the proteins were separated by SDS-PAGE. The tge BCA method was used for protein determination. SDS-PAGE was performed, and 10% gel was used for the SDS-PAGE assay. A total of 20 μg protein was loaded per lane. The proteins were sequentially transferred onto nitrocellulose (NC) membranes, followed by blocking with 5% fat-free milk in TBST buffer at room temperature for 2 h. The NC membranes were then incubated with primary antibodies to KIF4A, CDCA3, Ki67, PCNA, cyclin D1, cyclin A and β-actin at room temperature for 1.5 h. Subsequently the membranes were incubated with rabbit or mouse HRP-conjugated secondary antibodies (1:5,000 dilution, ab6721 for rabbit, and ab6728 for mouse; Abcam) at room temperature for 1 h. Signals were then visualized using an ECL kit (ab65623; Abcam). Signal intensity was measured using ImageJ (version 1.8.0; National Institutes of Health).

Colony formation assay

The BC cells, T24 and 5637, were transfected with the shRNA plasmids or overexpression plasmids for 48 h and then re-seeded into 6-well plates at a density of almost 500 cells each well and maintained for almost 2 weeks, when the colonies were formed. The colonies were then fixed with methanol at −20°C for 10 min and stained with 0.1% crystal violet (332488; Sigma-Aldrich; Merck KGaA) at room temperature for 20 min. After washing with PBS, the stained colonies were then photographed and the differences in colony numbers between the control- and KIF4A shRNA plasmid-transfected BC cells were calculated.

MTT assay

Both the T24 and 5637 cells were transfected with the shRNA plasmids or overexpression plasmids for 48 h and then seeded into the 96-well plates at a density of 1,000 cells each well and maintained for ~48 h. Cells were then treated with MTT (5 mg/ml, M2128; Sigma-Aldrich; Merck KGaA) for 4 h and washed with PBS 3 times. Cells were then stained by the use of 150 μl DMSO and the optical density (OD) value at a wavelength of 570 nm was measured and analyzed by a multifunctional enzyme label instrument (SpectraMax i3x; Molecular Devices).

CCK-8 assay

BC cells were plated into 96-well plates at a density of ~1,000 cells per well and subsequently transfected with the shRNA plasmids or overexpression plasmids for 48 h. The cells were then treated with CCK-8 (ab228554; Abcam) for 3 h and the absorbance value was measured at a wavelength of 490 nm by a multifunctional enzyme label instrument (SpectraMax i3x; Molecular Devices).

Cell cycle assay

Following transfection for 24 h, the cells were fixed using 70% ethyl alcohol for 24 h at −20°C and incubated with a concentration of 50 μg/ml propidium iodide (PI) at 37°C for 30 min, subsequently the samples were analyzed by the use of a FACSCalibur flow cytometer (FACSAria III; BD Biosciences). The percentage of cells in different phases, including the G1, S and G2/M phases was compared between the control-transfected and KIF4A-depleted cells.

Tumor growth in vivo assay

Animal maintenance and operation in the present study were approved by the Institutional Animal Care Committee (IACC) of the First Hospital of Xi'an Jiaotong University. Female BALB/c nude mice (8 weeks old; weithing ~20 g) were supplied by Beijing Vital River Experimental Animal Technology Co., Ltd. Mice were fed with food and water ad libitum and were fed at specific pathogen-free conditions at 20°C, 60% humidity and alternating 12-h light/dark cycles. A total of 12 athymic nude mice were included in the control (n=6) and shRNA (n=6) groups. The mice were sacrificed by excessive anesthesia via intraperitoneal injection with pentobarbital sodium at a concentration of 120 mg/kg. The hearts of the mice were then monitored and their deaths were confirmed by cardiac arrest. To measure tumor growth capacity in vivo, ~1×106 T24 cells were stably transfected with KIF4A-targeted shRNA plasmids to stably deplete the expression of KIF4A and injected subcutaneously into the right flanks of female nude mice (6 for each group). After 2 weeks, tumors began to form, and the volume was measured up to 7 weeks. The tumor volume was calculated as follows: Length × (width2)/2. The tumor growth curves were calculated and compared between the two groups.

ChIP and luciferase assays

ChIP assays were performed using a commercial kit, the ChIP assay kit (ab500; Abcam). A total number of 108 T24 cells were crosslinked, resuspended and lysed with RIPA buffer (R0278; Sigma-Aldrich; Merck KGaA), then sonicated so as to shear the DNA into a range of 500-1,000 bp. Chromatin fraction was then immunoprecipitated by the use of KIF4A or IgG (ab172730; Abcam, 1:200 dilution) antibodies at 4°C for 6 h, respectively, and the mix was enriched by the use of protein A Agarose (5015979001; Roche Diagnostics). Beads were isolated and washed with PBS 5 times. DNA was finally purified and RT-qPCR assays were performed as described above.

For luciferase assays, T24 cells were maintained and transfected with 1 μg pGL-CDCA3, pGL-CDCA1, pGL-CDCA8, pGL-Basic pEnter-KIF4A overexpression plasmids overnight by 5 μl Lipofectamine®. The luciferase reporter plasmid was bought from Merck (SRE0045). At 1 day following transfection, the cells were washed with PBS twice and lysed with RIPA (R0278; Sigma-Aldrich; Merck KGaA), and the luciferase activities were detected by the use of a luciferase assay kit (ab253393; Abcam). The normalization was performed by comparison with Renilla luciferase activity.

Statistical analysis

GraphPad 6.0 software was used for all statistical analyses in the present study. Data are represented as the mean ± SEM. Paired t-tests was used to compare the expression of KIF4A in tumor tissues and normal tissues. One-way ANOVA with Tukey's post hoc test were used to make comparisons among multiple groups. Moreover, the analysis of the association between the clinicopathological features of patients with BC and KIF4A expression was performed using the χ2 test. Kaplan-Meier survival analysis was performed to evaluate the prognosis of patients and the log rank test was used to determine statistically significant differences. Pearson's correlation analysis was also performed to determine the correlation between KIF4A and CDCA3 expression. The unpaired t-test was used for statistical comparisons, and P<0.05 was considered to indicate a statistically significant difference.

Results

KIF4A is highly expressed in human BC tissues and is associated with the prognosis of patients with BC

As a member of kinesin proteins, KIF4A can mediate the process of mitosis and tumorigenesis (16). The possible role of KIF4A on the progression of BC remains unclear. Therefore, KIF4A expression in BC tissues obtained from surgery and the corresponding normal tissues isolated from 159 patients was measured by IHC.

It was noted that KIF4A was mainly expressed in the nucleus and exhibited a brown-yellow granular appearance. The expression level of KIF4A was graded according to the staining intensity and the staining area. According to the staining results, it was found that KIF4A was located in the nucleus of cancer cells, and highly expressed in BC tissues, compared to the adjacent normal tissues (Fig. 1A). In addition, the mRNA levels of KIF4A were detected in 30 tumor tissues and corresponding normal tissues from patients with BC. Through RT-qPCR assays, it was found that the mRNA levels of KIF4A were markedly upregulated in the tumor tissues, consistent with data presented in Fig. 1A (Fig. 1B). Similarly, western blot analysis revealed the high expression of KIF4A in tumor tissues compared with normal tissues (Fig. 1C).

The mRNA levels of KIF4A was also significantly high in a total of 404 tumor tissues, compared with that in 28 normal tissues, which was analyzed using TCGA data (Fig. 1D). To further explore the effects of KIF4A on the progression of BC, the effects of KIF4A on the prognosis of BC were evaluated. Through Kaplan-Meier survival analysis using TCGA data, it was found that the mRNA level of KIF4A was evidently associated with the disease-free survival rate of two sets of patients with BC (Fig. 1E). However, no association was found between KIH4A and the overall survival of patients (data not shown). Collectively, these data demonstrated that KIF4A expression was associated with the prognosis of patients with BC.

Link between KIF4A expression and the clinicopathological characteristics of patients with BC

The potential link between the clinicopathological features of the 159 patients with BC and KIF4A expression was further assessed. Patient age, sex, tumor stage, grade and lymph node metastasis were analyzed, respectively. Of note, it was found that KIF4A expression was positively associated with tumor stage (P=0.012, Table I), whereas no significant associations were found between the expression of KIF4A and other clinicopathological features, including patient age (P=0.360), sex (P=0.566), tumor grade (P=0.236) and lymph node metastasis (P=0.889) (Table I).

Table I

Association of KIF4A and clinicopathological characteristics of 159 patients with BC.

Table I

Association of KIF4A and clinicopathological characteristics of 159 patients with BC.

FeatureAll n=159KIF4A expression
χ2P-value
Low (n=58)High (n=101)
Age (years)0.8360.360
 <651194178
 ≥65401723
Sex0.3300.566
 Male873057
 Female722844
Tumor stage6.3620.012a
 T1-T2673235
 T3/T4922666
Tumor grade1.4070.236
 Low592534
 High1003367
Lymph node metastasis0.0200.889
 Yes511932
 No1083969

a Statistically significant (P<0.05). KIF4A, kinesin family member 4A; BC, bladder cancer.

Knockdown of KIF4A in BC cells leads to an impaired capacity of proliferation in vitro

The present study then explored whether KIF4A affects the proliferation of BC cells in vitro. To explore the role of KIF4A in cell proliferation, KIF4A shRNA plasmids were first used to knock down the expression of KIF4A in BC cells, and its effects on cell proliferation were then detected. In total, two types of BC cells were used in these assays, the T24 and 5637 cells. The decreased expression of KIF4A following shRNA transfection was examined by RT-qPCR and western blot analysis. As was expected, the results indicated that the KIF4A expression level was significantly decreased in KIF4A shRNA-transfected T24 and 5637 cells (Fig. S1A and B).

Subsequently, the effects of KIF4A on the proliferation of BC cells were detected through colony formation, MTT and CCK-8 assays. The results of colony formation assays revealed that the number of colonies formed was markedly decreased following the knockdown of KIF4A in T24 and 5637 cells (Fig. 2A). Similarly, the results of MTT assay revealed that the KIF4A-depleted T24 and 5637 cells exhibited an evidently decreased absorbance value at the 570 nm wavelength (Fig. 2B). Similarly, through CCK-8 assays, it was found that the depletion of KIF4A led to a decrease in the absorbance value at the 490 nm wavelength, suggesting the suppression of cell proliferation induced by the knockdown of KIF4A (Fig. 2C).

To confirm the findings presented in Fig. 2A-C, the expression levels of 2 proliferative cell markers, PCNA and Ki67, were detected, respectively. A significant decrease in the PCNA and Ki67 expression levels was observed in the KIF4A-depleted BC cells, consistent with the decline in the cell proliferative capacity (Fig. 2D and E). Therefore, these data confirmed the promoting effects of KIF4A on BC cell proliferation.

KIF4A knockdown results in the arrest of the BC cell cycle

The cell cycle is known to be precisely controlled by various regulators to mediate cell proliferation. The present study then analyzed the differences in the cell cycle between the control and KIF4A-depleted T24 and 5637 cells. The results revealed that the depletion of KIF4A increased the percentage of T24 and 5637 cells in the S phase (Fig. S2A), suggesting the arrest of the cell cycle. In addition, the expression of the cell cycle markers, cyclin D1 and cyclin A was detected by western blot analysis, and the results revealed the evident decrease in cyclin D1 and cyclin A expression in the KIF4A-depleted T24 and 5637 cells, consistent with the aforementioned results shown in in Fig. S2A (Fig. S2B). On the whole, these data demonstrated that KIF4A depletion resulted in cell cycle arrest in BC.

KIF4A promotes the tumor growth of BC cells in vivo

The aforementioned results (Figs. 2 and S2) provided the evidence that KIF4A promoted cell proliferation and affected the cell cycle of BC in vitro. To further investigate whether KIF4A knockdown can suppress tumor growth, in vivo animal assays were performed. T24 cells stably transfected with control or KIF4A shRNA plasmids were injected into nude mice. After 2 weeks, tumors began to form. A total of five representative tumor samples in each group were photographed and are shown in Fig. 3A. After 7 weeks, the tumors were isolated, tumor volumes were compared, and the growth curves were calculated. From the tumor growth curves, it was noted that the tumors in the KIF4A-depleted groups were markedly smaller than those of the control group (Fig. 3A). To confirm the effective decrease, KIF4A expression was detected in tumor tissues, and the results of both western blot analysis and ICH revealed that the expression of KIF4A in the tumors derived from KIF4A-depleted cells was markedly decreased compared with that in the controls (Fig. 3B and C). Collectively, these results indicated that KIF4A promoted the tumor growth of BC cells in mice.

KIF4A promotes BC development by promoting the expression of CDCA3

As it was determined that KIF4A regulated cell proliferation in BC both in vitro and in vivo, the regulatory mechanisms underlying the promoting effects of KIF4A on BC cell proliferation were then investigated.

The aforementioned results (Fig. S2) indicated that the depletion of KIF4A significantly led to cell cycle arrest in BC. Through the analysis of TCGA data, it was found that the CDCA family members CDCA1, CDCA3 and CDCA8, which are key regulators of the cell cycle, were significantly highly expressed in BC tissues. Through TCGA data, it was further found that KIF4A expression significantly and positively correlated with the expression of CDCA1 (Fig. S3A, left panel), CDCA3 (Fig. 4A) and CDCA8 (Fig. S3A, right panel) in BC, suggesting that KIF4A regulates the expression of these proteins.

Importantly, by performing luciferase assays, the present study further examined whether KIF4A can transcriptionally promote CDCA3 expression. The pGL-CDCA3 plasmid containing the promoter region of CDCA3 was co-transfected with pEnter-vector or pEnter-KIF4A plasmid into T24 cells. According to the results, it was show that KIF4A could promote the activation of CDCA3 promoter in T24 cells (Fig. 4B). However, it was noted that KIF4A could not promote the activation of CDCA1 and CDCA8 (Fig. S3B). Of note, through ChIP assays, it was found that KIF4A antibody could be specifically co-immunoprecipitated by the promoter fragment of CDCA3 in T24 cells. Moreover, IgG antibody could not be co-immunoprecipitated, suggesting the specific binding of KIF4A with CDCA3 promoter (Fig. 4C). It was also found that KIF4A could not bind with the promoter of CDCA1 and CDCA8 (Fig. S3C). It was thus determined that KIF4A could not regulate the expression of CDCA1 and CDCA8.

Subsequently, RT-qPCR and western blot analysis were further conducted to detect the expression levels of CDCA3 in both KIF4A overexpression and depleted T24 cells. The over- expression of CDCA3 and KIF4A in T24 cells was observed (Fig. S4). In addition, the knockdown of KIF4A decreased the expression level of CDCA3 at the mRNA and protein level, respectively (Fig. 4D and E).

To further explore whether KIF4A promotes the proliferation of BC cells through the transcriptional activation of CDCA3, rescue assays were then performed. Through colony formation assays, it was revealed that the inhibition of cell proliferation induced by KIF4A knockdown was evidently reversed by CDCA3 overexpression (Fig. 4F). Additionally, CDCA3 overexpression following KIF4A depletion reversed the suppression of the proliferation of T24 and 5637 cells, which was confirmed through MTT assays (Fig. 4G). Collectively, these data demonstrate that KIF4A transcriptionally activates the expression of CDCA3.

KIF4A cooperates with CDCA3 to promote the growth of BC

The aforementioned results presented in Fig. 4 revealed that KIF4A could bind to the CDCA3 promoter and transactivate CDCA3, further promoting the proliferation of BC cells. The present study then aimed to confirm this finding using clinical samples. IHC assays were performed using the tumor tissue samples surgically obtained from human BC tissues, and the expression levels of both KIF4A and CDCA3 were detected and analyzed in continuous slices of tumor tissues. Notably, it was found that the expression of CDCA3 was mainly located in the cytoplasm and evidently associated with the expression of KIF4A (P<0.001, Fig. 5 and Table II). Based on these results, it was determined that KIF4A promotes the progression of BC through the transcriptional activation of CDCA3.

Table II

Association between KIF4A and CDCA3 expression in 159 patients with BC.

Table II

Association between KIF4A and CDCA3 expression in 159 patients with BC.

All patients (n=159)KIF4A
χ2P-value
Low (n=58)High (n=101)
CDCA318.849<0.001
Low (n=71)3932
High (n=88)1969

Discussion

Over the years, targeted therapy for BC has attracted increasing attention with its numerous advantages (28). Due to the high metastasis of cancer, advancements in targeted therapy are urgently required (29-31). At present, multiple molecular markers of BC, which have potential value for the diagnosis and prognosis of BC, have already been revealed (32). To improve the prognosis of patients with BC, novel therapeutic targets still need to be developed. Importantly, the present study found that a member of kinesins, KIF4A, was associated with the poor prognosis of patients with BC. KIF4A was also associated with the clinical features of patients with BC. Furthermore, KIF4A promoted BC cell proliferation in vitro and in vivo by transactivating CDCA3 (Fig. 6). Therefore, KIF4A may be considered as a novel therapeutic target for BC.

KIF4A is known to be involved in the regulation of chromosome congression and spindle dynamics during mitosis (33,34). In the present study, it was found KIF4A that affected the proliferation of BC cells in vitro and in mice, which was largely due to the effects on chromosomes and spindle. Another study demonstrated that KIF4A depletion led to the defects of chromosome formation and spindle organization, subsequently resulting in cell cycle arrest, consistent with our conjecture (18).

The effects of KIF4A on the cell cycle have been extensively studied in cancer cells. KIF4A ablation has been shown to cause the activation of SAC and further lead to cell cycle arrest at the G2/M phase (35). Another study indicated that HCC cells were arrested in the G2/M phase caused by KIF4A knockdown (36). Similarly, the present study found that KIF4A depletion blocked the BC cell cycle. Of note, it was also found that KIF4A promoted the transcription of CDCA3, a cell cycle regulator, and contributed to the control of cell cycle in BC cells, which may affect the growth of BC. Notably, KIF4A may also prevent cell apoptosis and thus promote tumorigenesis (36). Whether KIF4A regulates the development of BC by affecting cell apoptosis is also worthy of further study.

As is known, KIF4A serves as an oncogene and is involved in multiple types of tumors, such as breast, colorectal and oral cancer (20,37,38). KIF4A overexpression blocks cancer cell growth in the stomach (39). In breast cancer, KIF4A could mediate the Rad51 pathway through the interact with BRCA2 (40). Similarly, an increased KIF4A expression level has also been considered as a potential clinical and prognostic biomarker in prostate cancer (41). The present study demonstrated that KIF4A was associated with a poor prognosis of BC, and affected the progression of BC by regulating proliferation. The present study, together with other studies on KIF4A in cancer development, indicated the oncogene role of KIF4A in multiple types of cancer. However, the specific effects of KIF4A among different tumors also require further investigation.

In the present study, it was found that the CDCA family proteins, CDCA1, CDCA3 and CDCA8, were highly expressed in BC tissues according to TCGA data. Further analyses confirmed that the expression of KIF4A positively correlated with CDCA1, CDCA3 and CDCA8 expression in BC tissues. However, through ChIP and luciferase assays, it was found that only CDCA3 could be regulated by KIF4A. Therefore, these findings confirmed that KIF4A could affect the progression of BC by regulating CDCA3 expression.

Previous research has demonstrated that CDCA3 expression is related to an improved overall survival of patients with BC (42). Notably, it was found that KIF4A activated the expression of CDCA3, and further promoted the development of BC. In fact, it has been confirmed that kinesins cab transcriptional regulate CDCA family protein expression and promote cancer progression. For example, KIF18B has been shown to promote the proliferation of pancreatic ductal adenocarcinoma (PDAC) via activating CDCA8 expression (43).

Apart from KIF4A, several proteins promote cancer development by transcriptionally activating CDCA3. HoxB3 can promote prostate cancer progression via the promotion of CDCA3 expression (44). As a cell cycle regulator, CDCA3 also promotes the development of cancer progression, such as oral cancer (45). CDCA3 is also high expression in BC tissues. A previous study indicated that CDCA3 promoted the proliferation of colorectal cancer cells via the activation of the cyclin D1 signaling pathway (46). The present study screened CDCA3 as a downstream protein of KIF4A through ChIP assays; further molecular mechanisms need to be studied in view of its role in BC development. In the future, the authors aim to examine the mechanisms through which KIF4A further influences the BC cell cycle and proliferation by regulating CDCA3 transcription.

There are several other factors which are involved in the progression of BC. For example, CDODA-Me has been shown to decrease specificity protein transcription factors and to exhibit anti-neoplastic activity in bladder cancer cells by inducing reactive oxygen species (ROS) (47). Another study demonstrated the importance of the microenvironment of BC, providing evidence that the predominance of M2-polarized macrophages affected the angiogenesis, tumor grade and invasiveness of BC (48). The present study found that KIF4A plays a role in BC progression. However, it should also be determined whether KIF4A affects ROS and the microenvironment, and the underling mechanisms need to be elucidated. Notably, the present study demonstrated the effects of KIF4A on the apoptosis of BC cells. Further studies are required however, to detect the expression of p70S6K to investigate the capacity of BC cells to form spheres following apoptosis, according to a previous study (49).

In conclusion, the present study found that KIF4A was highly expressed in human BC tissues and was associated with the prognosis and clinicopathological characteristics of patients. It was also confirmed that KIF4A promoted the proliferation of BC cells in vitro and in mice in vivo. Furthermore, KIF4A transcriptionally activated the expression of CDCA3, and therefore contributed to the progression of BC. Therefore, KIF4A may prove to be a promising molecular target for the treatment of BC.

Supplementary Data

Availability of data and materials

The datasets used and/or analyzed during the present study are available from the author on reasonable request.

Authors' contributions

PZ, KWu, ZG and ZL performed the molecular biology experiments and drafted the manuscript. HL, WL, XW, ZS, FX, KWa and QH participated in the design of the study and performed the statistical analysis. PZ, KWu and ZL conceived the study, participated in the study design and coordination, and assisted in the drafting of the manuscript. PZ and ZL confirmed the authenticity of all the raw data. All authors have read and approved the final manuscript.

Ethics approval and consent to participate

All experiments involving human tissue were approved by the IACC of the First Hospital of Xi'an Jiaotong University (approval no. 2020-03-B003). Animal maintenance and operation in this study was approved by the Institutional Animal Care Committee (IACC) of the First Hospital of Xi'an Jiaotong University.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Acknowledgments

Not applicable.

Funding

No funding was received.

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June-2021
Volume 47 Issue 6

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Spandidos Publications style
Zheng P, Wu K, Gao Z, Li H, Li W, Wang X, Shi Z, Xiao F, Wang K, Li Z, Li Z, et al: KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3 Retraction in /10.3892/ijmm.2023.5265. Int J Mol Med 47: 99, 2021
APA
Zheng, P., Wu, K., Gao, Z., Li, H., Li, W., Wang, X. ... Han, Q. (2021). KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3 Retraction in /10.3892/ijmm.2023.5265. International Journal of Molecular Medicine, 47, 99. https://doi.org/10.3892/ijmm.2021.4932
MLA
Zheng, P., Wu, K., Gao, Z., Li, H., Li, W., Wang, X., Shi, Z., Xiao, F., Wang, K., Li, Z., Han, Q."KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3 Retraction in /10.3892/ijmm.2023.5265". International Journal of Molecular Medicine 47.6 (2021): 99.
Chicago
Zheng, P., Wu, K., Gao, Z., Li, H., Li, W., Wang, X., Shi, Z., Xiao, F., Wang, K., Li, Z., Han, Q."KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3 Retraction in /10.3892/ijmm.2023.5265". International Journal of Molecular Medicine 47, no. 6 (2021): 99. https://doi.org/10.3892/ijmm.2021.4932