Open Access

Role of microRNA‑375‑3p‑mediated regulation in tinnitus development

  • Authors:
    • Kyu-Hee Han
    • Hyeeun Cho
    • Kyeo-Rye Han
    • Seog-Kyun Mun
    • Young-Kook Kim
    • Ilyong Park
    • Munyoung Chang
  • View Affiliations

  • Published online on: May 21, 2021     https://doi.org/10.3892/ijmm.2021.4969
  • Article Number: 136
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Changes in the dorsal cochlear nucleus (DCN) following exposure to noise play an important role in the development of tinnitus. As the development of several diseases is known to be associated with microRNAs (miRNAs/miRs), the aim of the present study was to identify the miRNAs that may be implicated in pathogenic changes in the DCN, resulting in tinnitus. A previously developed tinnitus animal model was used for this study. The study consisted of four stages, including identification of candidate miRNAs involved in tinnitus development using miRNA microarray analysis, validation of miRNA expression using reverse transcription‑quantitative PCR (RT‑qPCR), evaluation of the effects of candidate miRNA overexpression on tinnitus development through injection of a candidate miRNA mimic or mimic negative control, and target prediction of candidate miRNAs using mRNA microarray analysis and western blotting. The miRNA microarray and RT‑qPCR analyses revealed that miR‑375‑3p expression was significantly reduced in the tinnitus group compared with that in the non‑tinnitus group. Additionally, miR‑375‑3p overexpression via injection of miR‑375‑3p mimic reduced the proportion of animals with persistent tinnitus. Based on mRNA microarray and western blot analyses, connective tissue growth factor (CTGF) was identified as a potential target for miR‑375‑3p. Thus, it was inferred that CTGF downregulation by miR‑375‑3p may weaken with the decrease in miRNA expression, and the increased pro‑apoptotic activity of CTGF may result in more severe neuronal damage, contributing to tinnitus development. These findings are expected to contribute significantly to the development of a novel therapeutic approach to tinnitus, thereby bringing about a significant breakthrough in the treatment of this potentially debilitating condition.
View Figures
View References

Related Articles

Journal Cover

July-2021
Volume 48 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Han K, Cho H, Han K, Mun S, Kim Y, Park I and Chang M: Role of microRNA‑375‑3p‑mediated regulation in tinnitus development. Int J Mol Med 48: 136, 2021
APA
Han, K., Cho, H., Han, K., Mun, S., Kim, Y., Park, I., & Chang, M. (2021). Role of microRNA‑375‑3p‑mediated regulation in tinnitus development. International Journal of Molecular Medicine, 48, 136. https://doi.org/10.3892/ijmm.2021.4969
MLA
Han, K., Cho, H., Han, K., Mun, S., Kim, Y., Park, I., Chang, M."Role of microRNA‑375‑3p‑mediated regulation in tinnitus development". International Journal of Molecular Medicine 48.1 (2021): 136.
Chicago
Han, K., Cho, H., Han, K., Mun, S., Kim, Y., Park, I., Chang, M."Role of microRNA‑375‑3p‑mediated regulation in tinnitus development". International Journal of Molecular Medicine 48, no. 1 (2021): 136. https://doi.org/10.3892/ijmm.2021.4969