Open Access

Exhaustion‑like dysfunction of T and NKT cells in an X‑linked severe combined immunodeficiency patient with maternal engraftment by single‑cell analysis

  • Authors:
    • Wei Dong
    • Wenyan Li
    • Shaojin Zhang
    • Xian Zeng
    • Qi Qin
    • Huifeng Fan
    • Zhonghui Tang
    • Xia Wu
    • Gen Lu
  • View Affiliations

  • Published online on: February 6, 2023     https://doi.org/10.3892/ijmm.2023.5228
  • Article Number: 25
  • Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Maternal engraftment is frequently present in X‑linked severe combined immunodeficiency (X‑SCID) patients caused by pathogenic mutations in IL2GR. However, the functional status of the engrafted cells remains unclear because of the difficulty in separately evaluating the function of the maternal and autologous cells. The present study reported an X‑SCID patient with a de novo c.677C>T (p.R226H) variant in exon 5 of IL2RG, exhibiting recurrent and persistent infections from 3‑months‑old. After the male patient suffering recurrent pneumonia and acute hematogenous disseminated tuberculosis when 13‑months‑old, single‑cell RNA sequencing was applied to characterize the transcriptome landscape of his bone marrow mononuclear cells (BMMNCs). A novel bioinformatic analysis strategy was designed to discriminate maternal and autologous cells at single‑cell resolution. The maternal engrafted cells consisted primarily of T, NKT and NK cells and the patient presented with the coexistence of autologous cells of these cell types. When compared respectively with normal counterparts, both maternal and autologous T and NKT cells increased the transcription of some important cytokines (GZMB, PRF1 and NKG7) against infections, but decreased the expression of a number of key transcription factors (FOS, JUN, TCF7 and LEF1) related to lymphocyte activation, proliferation and differentiation. Notably, the expression of multiple inhibitory factors (LAG3, CTLA4 and HAVCR2) were substantially enhanced in the T and NKT cells of both origins. In conclusion, both maternal and autologous T and NKT cells exhibited exhaustion‑like dysfunction in this X‑SCID patient suffering recurrent and persistent infections.
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March-2023
Volume 51 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Dong W, Li W, Zhang S, Zeng X, Qin Q, Fan H, Tang Z, Wu X and Lu G: Exhaustion‑like dysfunction of T and NKT cells in an X‑linked severe combined immunodeficiency patient with maternal engraftment by single‑cell analysis. Int J Mol Med 51: 25, 2023
APA
Dong, W., Li, W., Zhang, S., Zeng, X., Qin, Q., Fan, H. ... Lu, G. (2023). Exhaustion‑like dysfunction of T and NKT cells in an X‑linked severe combined immunodeficiency patient with maternal engraftment by single‑cell analysis. International Journal of Molecular Medicine, 51, 25. https://doi.org/10.3892/ijmm.2023.5228
MLA
Dong, W., Li, W., Zhang, S., Zeng, X., Qin, Q., Fan, H., Tang, Z., Wu, X., Lu, G."Exhaustion‑like dysfunction of T and NKT cells in an X‑linked severe combined immunodeficiency patient with maternal engraftment by single‑cell analysis". International Journal of Molecular Medicine 51.3 (2023): 25.
Chicago
Dong, W., Li, W., Zhang, S., Zeng, X., Qin, Q., Fan, H., Tang, Z., Wu, X., Lu, G."Exhaustion‑like dysfunction of T and NKT cells in an X‑linked severe combined immunodeficiency patient with maternal engraftment by single‑cell analysis". International Journal of Molecular Medicine 51, no. 3 (2023): 25. https://doi.org/10.3892/ijmm.2023.5228