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August 2012 Volume 41 Issue 2

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Article

ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells

  • Authors:
    • Lian-Nv Qiu
    • Yong-Lie Zhou
    • Zhen-Ni Wang
    • Qiang Huang
    • Wei-Xiao Hu
  • View Affiliations / Copyright

    Affiliations: Centre of Laboratory Medicine, Zhejiang Provincial People's Hospital, Hangzhou 310014, P.R. China, Centre of Laboratory Medicine, Zhejiang Provincial People's Hospital, Hangzhou 310014, P.R. China, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, P.R. China
  • Pages: 533-540
    |
    Published online on: May 8, 2012
       https://doi.org/10.3892/ijo.2012.1467
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Abstract

Chronic lymphocytic leukemia (CLL) is a low-grade lymphoid malignancy incurable with conventional modalities of chemotherapy. We aimed to examine the proapoptotic effects of a novel proteasome inhibitor, N,N'-di-(m-methylphenyi)-3,6- dimethyl-1,4-dihydro-1,2,4,5-tetrazine-1,4-dicarboamide (ZGDHu-1), on CLL cells. B lymphocytes were isolated from CLL patients and normal healthy controls, and treated with various concentrations of ZGDHu-1 for different days. CLL cell viability was detected by MTT assay. The apoptosis, mitochondrial membrane potential (∆ψm) and reactive oxidative species (ROS) were examined by flow cytometry. The expression of caspase-3 and Bcl-2/Bax ratio was detected by western blotting. ZGDHu-1 significantly reduced the viability of CLL cells and induced apoptosis in comparison to the control cells (both P<0.05). Normal peripheral B cells were resistant to the apoptosis-inducing effects of ZGDHu-1. Apoptosis induced by ZGDHu-1 was accompanied with generation of ROS, loss of ∆ψm, downregulation of Bcl-2 and increase of caspase-3 cleavage. Results of this study indicate that ZGDHu-1 is a promising specific treatment for CLL in the clinic.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu L, Zhou Y, Wang Z, Huang Q and Hu W: ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells. Int J Oncol 41: 533-540, 2012.
APA
Qiu, L., Zhou, Y., Wang, Z., Huang, Q., & Hu, W. (2012). ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells. International Journal of Oncology, 41, 533-540. https://doi.org/10.3892/ijo.2012.1467
MLA
Qiu, L., Zhou, Y., Wang, Z., Huang, Q., Hu, W."ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells". International Journal of Oncology 41.2 (2012): 533-540.
Chicago
Qiu, L., Zhou, Y., Wang, Z., Huang, Q., Hu, W."ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells". International Journal of Oncology 41, no. 2 (2012): 533-540. https://doi.org/10.3892/ijo.2012.1467
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu L, Zhou Y, Wang Z, Huang Q and Hu W: ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells. Int J Oncol 41: 533-540, 2012.
APA
Qiu, L., Zhou, Y., Wang, Z., Huang, Q., & Hu, W. (2012). ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells. International Journal of Oncology, 41, 533-540. https://doi.org/10.3892/ijo.2012.1467
MLA
Qiu, L., Zhou, Y., Wang, Z., Huang, Q., Hu, W."ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells". International Journal of Oncology 41.2 (2012): 533-540.
Chicago
Qiu, L., Zhou, Y., Wang, Z., Huang, Q., Hu, W."ZGDHu-1 promotes apoptosis of chronic lymphocytic leukemia cells". International Journal of Oncology 41, no. 2 (2012): 533-540. https://doi.org/10.3892/ijo.2012.1467
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