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Article

Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases

  • Authors:
    • Ketao Jin
    • Huanrong Lan
    • Feilin Cao
    • Na Han
    • Zhenzhen  Xu
    • Guangliang Li
    • Kuifeng He
    • Lisong Teng
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical College, Linhai, Zhejiang, P.R. China, Department of Gynecology and Obstetrics, Taizhou Hospital, Wenzhou Medical College, Linhai, Zhejiang, P.R. China, Cancer Chemotherapy Center, Zhejiang Cancer Hospital, Zhejiang University of Chinese Medicine, Hangzhou, Zhejiang, P.R. China, Department of Surgical Oncology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, P.R. China, Department of Surgical Oncology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, P.R. China
  • Pages: 583-588
    |
    Published online on: May 10, 2012
       https://doi.org/10.3892/ijo.2012.1469
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Abstract

Heterogeneity in primary tumors and related metastases may result in failure of antitumor therapies, particularly in targeted therapies for the treatment of cancer. In this study, patient-derived tumor tissue (PDTT) xenograft models of colon carcinoma with lymphatic and hepatic metastases were used to evaluate the response to EGFR- and VEGF-targeted therapies. Our results showed that primary colon carcinoma and its corresponding lymphatic and hepatic metastases have a different response rate to anti-EGFR (cetuximab) and anti-VEGF (bevacizumab) therapies. However, the underlying mechanism of these types of phenomenon is still unclear. To investigate whether such phenomena may result from the heterogeneity in primary colon carcinoma and related metastases, we compared the expression levels of cell signaling pathway proteins using immunohistochemical staining and western blotting, and the gene status of KRAS using pyrosequencing in the same primary colon carcinoma and its corresponding lymphatic and hepatic metastatic tissues which were used for establishing the PDTT xenograft models. Our results showed that the expression levels of EGFR, VEGF, Akt/pAkt, ERK/pERK, MAPK/pMAPK, and mTOR/pmTOR were different in primary colon carcinoma and matched lymphatic and hepatic metastases although the KRAS gene status in all cases was wild-type. Our results indicate that the heterogeneity in primary colon carcinoma and its corresponding lymphatic and hepatic metastases may result in differences in the response to dual-inhibition of EGFR and VEGF.
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Copy and paste a formatted citation
Spandidos Publications style
Jin K, Lan H, Cao F, Han N, Xu Z, Li G, He K and Teng L: Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases. Int J Oncol 41: 583-588, 2012.
APA
Jin, K., Lan, H., Cao, F., Han, N., Xu, Z., Li, G. ... Teng, L. (2012). Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases. International Journal of Oncology, 41, 583-588. https://doi.org/10.3892/ijo.2012.1469
MLA
Jin, K., Lan, H., Cao, F., Han, N., Xu, Z., Li, G., He, K., Teng, L."Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases". International Journal of Oncology 41.2 (2012): 583-588.
Chicago
Jin, K., Lan, H., Cao, F., Han, N., Xu, Z., Li, G., He, K., Teng, L."Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases". International Journal of Oncology 41, no. 2 (2012): 583-588. https://doi.org/10.3892/ijo.2012.1469
Copy and paste a formatted citation
x
Spandidos Publications style
Jin K, Lan H, Cao F, Han N, Xu Z, Li G, He K and Teng L: Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases. Int J Oncol 41: 583-588, 2012.
APA
Jin, K., Lan, H., Cao, F., Han, N., Xu, Z., Li, G. ... Teng, L. (2012). Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases. International Journal of Oncology, 41, 583-588. https://doi.org/10.3892/ijo.2012.1469
MLA
Jin, K., Lan, H., Cao, F., Han, N., Xu, Z., Li, G., He, K., Teng, L."Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases". International Journal of Oncology 41.2 (2012): 583-588.
Chicago
Jin, K., Lan, H., Cao, F., Han, N., Xu, Z., Li, G., He, K., Teng, L."Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases". International Journal of Oncology 41, no. 2 (2012): 583-588. https://doi.org/10.3892/ijo.2012.1469
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