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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2012 Volume 41 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells

  • Authors:
    • Chang G. Dong
    • William K.K. Wu
    • Su Y. Feng
    • Xiao J. Wang
    • Jun F. Shao
    • Jian Qiao
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, P.R. China, Institute of Digestive Disease, Department of Medicine and Therapeutics and LKS Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, P.R. China, Department of Neurosurgery, Wuxi No. 4 People's Hospital affiliated to Soochow University, Wuxi 214062, P.R. China, Institute of Digestive Disease, Department of Medicine and Therapeutics and LKS Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, P.R. China, Department of Neurosurgery, Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, P.R. China, Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, P.R. China
  • Pages: 1005-1012
    |
    Published online on: July 3, 2012
       https://doi.org/10.3892/ijo.2012.1542
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Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that function as negative gene regulators. Alterations in the expression of miRNAs have been implicated in the pathogenesis and development of most human malignancies. Recent data indicate that microRNA-21 and microRNA-10b are significantly elevated in glioblastoma multiforme (GBM) suggesting their role in the regulation of multiple genes associated with cancer. In this study, U87MG human glioblastoma cells were treated with miRNA inhibitors targeting miR-10b and miR-21, alone or in combination. The results showed that the miR-21 inhibitor additively interacted with miR-10b inhibitor on U87MG cells. The 50% inhibitory concentration values were dramatically decreased in cells treated with the combination of miR-10b and miR-21 inhibitors. Furthermore, inhibitors synergistically combined, enhanced apoptosis significantly and reduced invasion ability assessed by flow cytometry and Transwell migration assay. Thus, the miR-21 inhibitor may interrupt the activity of EGFR pathways, increasing PDCD4 and TPM1 expression and reducing MMP activities, independently of PTEN status. Meanwhile, miR-10b inhibitor reduced by Twist proceeds to inhibit translation of the mRNA encoding HOXD10 leading to the increase of the expression of the well-characterized pro-metastatic gene RHOC. Taken together, these data strongly suggest that a combination of miR-21 inhibitor and miR-10b inhibitor could be an effective therapeutic strategy for controlling the growth of GBM by inhibiting oncogene expression and overexpressing tumor suppressor genes. Moreover, a regulatory strategy based on the combination of miRNA inhibitors may provide insights into the mechanisms of the modulation of signaling genes involved in tumor cell apoptosis and invasiveness.
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Copy and paste a formatted citation
Spandidos Publications style
Dong CG, Wu WK, Feng SY, Wang X , Shao JF and Qiao J: Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells. Int J Oncol 41: 1005-1012, 2012.
APA
Dong, C.G., Wu, W.K., Feng, S.Y., Wang, X. ., Shao, J.F., & Qiao, J. (2012). Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells. International Journal of Oncology, 41, 1005-1012. https://doi.org/10.3892/ijo.2012.1542
MLA
Dong, C. G., Wu, W. K., Feng, S. Y., Wang, X. ., Shao, J. F., Qiao, J."Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells". International Journal of Oncology 41.3 (2012): 1005-1012.
Chicago
Dong, C. G., Wu, W. K., Feng, S. Y., Wang, X. ., Shao, J. F., Qiao, J."Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells". International Journal of Oncology 41, no. 3 (2012): 1005-1012. https://doi.org/10.3892/ijo.2012.1542
Copy and paste a formatted citation
x
Spandidos Publications style
Dong CG, Wu WK, Feng SY, Wang X , Shao JF and Qiao J: Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells. Int J Oncol 41: 1005-1012, 2012.
APA
Dong, C.G., Wu, W.K., Feng, S.Y., Wang, X. ., Shao, J.F., & Qiao, J. (2012). Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells. International Journal of Oncology, 41, 1005-1012. https://doi.org/10.3892/ijo.2012.1542
MLA
Dong, C. G., Wu, W. K., Feng, S. Y., Wang, X. ., Shao, J. F., Qiao, J."Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells". International Journal of Oncology 41.3 (2012): 1005-1012.
Chicago
Dong, C. G., Wu, W. K., Feng, S. Y., Wang, X. ., Shao, J. F., Qiao, J."Co-inhibition of microRNA-10b and microRNA-21 exerts synergistic inhibition on the proliferation and invasion of human glioma cells". International Journal of Oncology 41, no. 3 (2012): 1005-1012. https://doi.org/10.3892/ijo.2012.1542
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