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Article

Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells

  • Authors:
    • Tetsuya Tamatani
    • Tarannum Ferdous
    • Natsumi Takamaru
    • Kanae Hara
    • Makoto Kinouchi
    • Nobuyuki Kuribayashi
    • Go Ohe
    • Daisuke Uchida
    • Hirokazu Nagai
    • Kenji Fujisawa
    • Youji Miyamoto
  • View Affiliations / Copyright

    Affiliations: Department of Oral Surgery, Subdivision of Molecular Oral Medicine, Division of Integrated Sciences of Translational Research, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8504, Japan
  • Pages: 1148-1156
    |
    Published online on: July 4, 2012
       https://doi.org/10.3892/ijo.2012.1544
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Abstract

Docetaxel (DOC) and 5-fluorouracil (5-FU) are important anticancer agents widely used in the treatment of a variety of cancers including oral squamous cell carcinoma (OSCC). The purpose of this study was to determine the antitumor efficacy of the sequential administration of DOC and 5-FU against OSCC cells (B88 and CAL27 cells) in vitro and in vivo. In in vitro growth inhibition assays, sequential treatment with DOC followed by 5-FU was more effective in inhibiting cancer cell growth than 5-FU followed by DOC, single treatment with DOC or 5-FU, or combined treatment with DOC and 5-FU. Furthermore, DOC followed by 5-FU significantly inhibited tumor growth in vivo compared to 5-FU followed by DOC. To understand the mechanisms underlying the enhanced growth inhibitory effect of the administration sequence, DOC followed by 5-FU, we examined the expression of 5-FU metabolic enzymes such as thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD) and orotate phosphoribosyl transferase (OPRT), which were known to regulate the antitumor effect of 5-FU, by real-time RT-PCR and western blot analysis. Downregulation of TS and DPD expression and upregulation of OPRT expression were induced by DOC treatment, suggesting that DOC enhanced the efficacy of 5-FU by altering the expression of its metabolic enzymes. These results indicate that sequential treatment with DOC followed by 5-FU could be a promising therapeutic strategy for oral cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Tamatani T, Ferdous T, Takamaru N, Hara K, Kinouchi M, Kuribayashi N, Ohe G, Uchida D, Nagai H, Fujisawa K, Fujisawa K, et al: Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells. Int J Oncol 41: 1148-1156, 2012.
APA
Tamatani, T., Ferdous, T., Takamaru, N., Hara, K., Kinouchi, M., Kuribayashi, N. ... Miyamoto, Y. (2012). Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells. International Journal of Oncology, 41, 1148-1156. https://doi.org/10.3892/ijo.2012.1544
MLA
Tamatani, T., Ferdous, T., Takamaru, N., Hara, K., Kinouchi, M., Kuribayashi, N., Ohe, G., Uchida, D., Nagai, H., Fujisawa, K., Miyamoto, Y."Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells". International Journal of Oncology 41.3 (2012): 1148-1156.
Chicago
Tamatani, T., Ferdous, T., Takamaru, N., Hara, K., Kinouchi, M., Kuribayashi, N., Ohe, G., Uchida, D., Nagai, H., Fujisawa, K., Miyamoto, Y."Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells". International Journal of Oncology 41, no. 3 (2012): 1148-1156. https://doi.org/10.3892/ijo.2012.1544
Copy and paste a formatted citation
x
Spandidos Publications style
Tamatani T, Ferdous T, Takamaru N, Hara K, Kinouchi M, Kuribayashi N, Ohe G, Uchida D, Nagai H, Fujisawa K, Fujisawa K, et al: Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells. Int J Oncol 41: 1148-1156, 2012.
APA
Tamatani, T., Ferdous, T., Takamaru, N., Hara, K., Kinouchi, M., Kuribayashi, N. ... Miyamoto, Y. (2012). Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells. International Journal of Oncology, 41, 1148-1156. https://doi.org/10.3892/ijo.2012.1544
MLA
Tamatani, T., Ferdous, T., Takamaru, N., Hara, K., Kinouchi, M., Kuribayashi, N., Ohe, G., Uchida, D., Nagai, H., Fujisawa, K., Miyamoto, Y."Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells". International Journal of Oncology 41.3 (2012): 1148-1156.
Chicago
Tamatani, T., Ferdous, T., Takamaru, N., Hara, K., Kinouchi, M., Kuribayashi, N., Ohe, G., Uchida, D., Nagai, H., Fujisawa, K., Miyamoto, Y."Antitumor efficacy of sequential treatment with docetaxel and 5-fluorouracil against human oral cancer cells". International Journal of Oncology 41, no. 3 (2012): 1148-1156. https://doi.org/10.3892/ijo.2012.1544
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