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Article Open Access

Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells

  • Authors:
    • María Luisa Hernández-Bule
    • Ernesto Roldán
    • Joaquín Matilla
    • María Ángeles Trillo
    • Alejandro Úbeda
  • View Affiliations / Copyright

    Affiliations: Department of Research-BEM-IRYCIS, Hospital Ramon y Cajal, 28034 Madrid, Spain, Clinical Immunology, Cellular and Tumoral Immunology Section, Hospital Ramon y Cajal, 28034 Madrid, Spain, Research-Biochemistry-IRYCIS, Hospital Ramon y Cajal, 28034 Madrid, Spain
    Copyright: © Hernández-Bule et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1251-1259
    |
    Published online on: July 24, 2012
       https://doi.org/10.3892/ijo.2012.1569
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Abstract

Recently, a number of electric and electrothermal therapies have been applied to the treatment of specific cancer types. However, the cellular and molecular mechanisms involved in the response to such therapies have not been well characterized yet. Capacitive-resistive electric transfer (CRET) therapy uses electric currents at frequencies within the 0.45-0.6 MHz range to induce hyperthermia in target tissues. Preliminary trials in cancer patients have shown consistent signs that CRET could slow down growth of tumor tissues in brain gliomas, without inducing detectable damage in the surrounding healthy tissue. Previous studies by our group have shown that subthermal treatment with 0.57-MHz electric currents can induce a cytostatic, not cytotoxic response in HepG2 human hepatocarcinoma cells; such effect being mediated by cell cycle alterations. In contrast, the study of the response of NB69 human neuroblastoma cells to the same electric treatment revealed consistent indications of cytotoxic effects. The present study extends the knowledge on the response of NB69 cells to the subthermal stimulus, comparing it to that of primary cultures of human peripheral blood mononuclear cells (PBMC) exposed to the same treatment. The results showed no sensitivity of PBMC to the 0.57 MHz subthermal currents and confirmed that the treatment exerts a cytotoxic action in NB69 cells. The data also revealed a previously undetected cytostatic response of the neuroblastoma cell line. CRET currents affected NB69 cell proliferation by significantly reducing the fraction of cells in the phase G2/M of the cell cycle at 12 h of exposure. These data provide new information on the mechanisms of response to CRET therapy, and are consistent with a cytotoxic and/or cytostatic action of the electric treatment, which would affect human cells of tumor origin but not normal cells with a low proliferation rate.
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Copy and paste a formatted citation
Spandidos Publications style
Hernández-Bule ML, Roldán E, Matilla J, Trillo MÁ and Úbeda A: Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells. Int J Oncol 41: 1251-1259, 2012.
APA
Hernández-Bule, M.L., Roldán, E., Matilla, J., Trillo, M.Á., & Úbeda, A. (2012). Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells. International Journal of Oncology, 41, 1251-1259. https://doi.org/10.3892/ijo.2012.1569
MLA
Hernández-Bule, M. L., Roldán, E., Matilla, J., Trillo, M. Á., Úbeda, A."Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells". International Journal of Oncology 41.4 (2012): 1251-1259.
Chicago
Hernández-Bule, M. L., Roldán, E., Matilla, J., Trillo, M. Á., Úbeda, A."Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells". International Journal of Oncology 41, no. 4 (2012): 1251-1259. https://doi.org/10.3892/ijo.2012.1569
Copy and paste a formatted citation
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Spandidos Publications style
Hernández-Bule ML, Roldán E, Matilla J, Trillo MÁ and Úbeda A: Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells. Int J Oncol 41: 1251-1259, 2012.
APA
Hernández-Bule, M.L., Roldán, E., Matilla, J., Trillo, M.Á., & Úbeda, A. (2012). Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells. International Journal of Oncology, 41, 1251-1259. https://doi.org/10.3892/ijo.2012.1569
MLA
Hernández-Bule, M. L., Roldán, E., Matilla, J., Trillo, M. Á., Úbeda, A."Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells". International Journal of Oncology 41.4 (2012): 1251-1259.
Chicago
Hernández-Bule, M. L., Roldán, E., Matilla, J., Trillo, M. Á., Úbeda, A."Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells". International Journal of Oncology 41, no. 4 (2012): 1251-1259. https://doi.org/10.3892/ijo.2012.1569
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