Surfactin suppresses TPA-induced breast cancer cell invasion through the inhibition of MMP-9 expression

  • Authors:
    • Sun Young Park
    • Ji-Hee Kim
    • Young Ji Lee
    • Sang Joon Lee
    • Younghee Kim
  • View Affiliations

  • Published online on: November 13, 2012     https://doi.org/10.3892/ijo.2012.1695
  • Pages: 287-296
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Abstract

Metastasis is the main cause of cancer mortality. In this study, we investigated the effects of surfactin, a cyclic lipopeptide produced by Bacillus subtilis, on cancer metastasis in vitro and the underlying molecular mechanisms involved. Surfactin inhibited the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced invasion, migration and colony formation of human breast carcinoma cells. Western blot analysis, gelatin zymography and reverse transcription-PCR analysis revealed that matrix metalloproteinase-9 (MMP-9) expression and activation was significantly suppressed by surfactin in a dose-dependent manner. Surfactin attenuated TPA-induced nuclear translocation and activation of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). Furthermore, surfactin strongly repressed the TPA-induced phosphorylation of Akt and extracellular signal-regulated kinase (ERK). Treatment with specific inhibitors of Akt and ERK suppressed MMP-9 expression and activation. These results suggest that the surfactin-mediated inhibition of breast cancer cell invasion and MMP-9 expression involves the suppression of the NF-κB, AP-1, phosphatidylinositol 3-kinase (PI-3K)/Akt and the ERK signaling pathways. Thus surfactin may have potential value in therapeutic strategies for the treatment of breast cancer metastasis.
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January 2013
Volume 42 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Park SY, Kim J, Lee YJ, Lee SJ and Kim Y: Surfactin suppresses TPA-induced breast cancer cell invasion through the inhibition of MMP-9 expression. Int J Oncol 42: 287-296, 2013
APA
Park, S.Y., Kim, J., Lee, Y.J., Lee, S.J., & Kim, Y. (2013). Surfactin suppresses TPA-induced breast cancer cell invasion through the inhibition of MMP-9 expression. International Journal of Oncology, 42, 287-296. https://doi.org/10.3892/ijo.2012.1695
MLA
Park, S. Y., Kim, J., Lee, Y. J., Lee, S. J., Kim, Y."Surfactin suppresses TPA-induced breast cancer cell invasion through the inhibition of MMP-9 expression". International Journal of Oncology 42.1 (2013): 287-296.
Chicago
Park, S. Y., Kim, J., Lee, Y. J., Lee, S. J., Kim, Y."Surfactin suppresses TPA-induced breast cancer cell invasion through the inhibition of MMP-9 expression". International Journal of Oncology 42, no. 1 (2013): 287-296. https://doi.org/10.3892/ijo.2012.1695