Distinct expression of C4.4A in colorectal cancer detected by different antibodies Corrigendum in /10.3892/ijo.2016.3694

Yamamoto,Hirofumi; Oshiro,Ryota; Ohtsuka,Masahisa; Uemura,Mamoru; Haraguchi,Naotsugu; Nishimura,Junichi; Takemasa,Ichiro; Mizushima,Tsunekazu; Doki,Yuichiro; Mori,Masaki

doi:10.3892/ijo.2012.1714

January 2013 Volume 42 Issue 1

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January 2013 Volume 42 Issue 1

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Article

Distinct expression of C4.4A in colorectal cancer detected by different antibodies

Corrigendum in: /10.3892/ijo.2016.3694

Authors:
- Hirofumi Yamamoto
- Ryota Oshiro
- Masahisa Ohtsuka
- Mamoru Uemura
- Naotsugu Haraguchi
- Junichi Nishimura
- Ichiro Takemasa
- Tsunekazu Mizushima
- Yuichiro Doki
- Masaki Mori
View Affiliations / Copyright

Affiliations: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
Pages: 197-201
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Published online on: November 22, 2012

https://doi.org/10.3892/ijo.2012.1714
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Abstract

The metastasis-associated gene C4.4A encodes a glycolipid-anchored membrane protein expressed in several human malignancies. The present study aimed to perform a detailed assessment of C4.4A expression in colorectal cancer tissues, in terms of intra-cellular localization, intra-tumoral location and difference in molecular weight. To advance this goal, we developed three new antibodies against the C4.4A protein (two polyclonal Abs: C4.4A-119 and C4.4A-277 and one monoclonal Ab: C4.4A GPI-M) to use in addition to the two previously produced polyclonal Abs (C4.4A-81, C4.4A GPI-P). Antibody specificities were confirmed by absorption tests. Western blot analysis and immunohistochemistry showed that the C4.4A-119 and C4.4-277 Abs detected 70-kDa C4.4A, mainly in the cytoplasm, irrespective of intra-tumoral location. The C4.4A GPI-P and C4.4A GPI-M Abs reacted with the membranous ~40-kDa C4.4A, exclusively at the tumor invasive front, and each detected an identical tumor cell population. The tested antibodies showed varied C4.4A detection rates in 33 CRC tissues. The C4.4A-277 Ab yielded the highest positive rate in 29 of 33 CRC tissues (87.9%), while the C4.4A GPI-P and C4.4A GPI-M Abs each only showed 33.3% positivity. The present findings suggest that the GPI anchor signaling sequence may be essential for detecting membranous C4.4A at the invasive front of CRC tissues.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Distinct expression of C4.4A in colorectal cancer detected by different antibodies

Corrigendum in: /10.3892/ijo.2016.3694

This article is mentioned in:

Abstract

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