Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells

Jiang,Yong-Sheng; Maeda,Masayo; Okamoto,Mayumi; Fujii,Mikiko; Fukutomi,Ryuichiro; Hori,Masato; Tatsuka,Masaaki; Ota,Takahide

doi:10.3892/ijo.2012.1730

Centrosomal localization of RhoGDIβ and its relevance to mitotic processes in cancer cells

Authors:
- Yong-Sheng Jiang
- Masayo Maeda
- Mayumi Okamoto
- Mikiko Fujii
- Ryuichiro Fukutomi
- Masato Hori
- Masaaki Tatsuka
- Takahide Ota
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Affiliations: Division of Tumor Biology, Department of Life Science, Medical Research Institute, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan, Department of Chemistry, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan, Department of Life Sciences, Life and Environmental Sciences, Prefectural University of Hiroshima, Shoubara, Hiroshima 727-0023, Japan
Published online on: December 7, 2012 https://doi.org/10.3892/ijo.2012.1730
Pages: 460-468
Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Rho GDP-dissociation inhibitors (RhoGDIs) are regulators of Rho family GTPases. RhoGDIβ has been implicated in cancer progression, but its precise role remains unclear. We determined the subcellular localization of RhoGDIβ and examined the effects of its overexpression and RNAi knockdown in cancer cells. Immunofluorescence staining showed that RhoGDIβ localized to centrosomes in human cancer cells. In HeLa cells, exogenous GFP-tagged RhoGDIβ localized to centrosomes and its overexpression caused prolonged mitosis and aberrant cytokinesis in which the cell shape was distorted. RNAi knockdown of RhoGDIβ led to increased incidence of monopolar spindle mitosis resulting in polyploid cells. These results suggest that RhoGDIβ has mitotic functions, including regulation of cytokinesis and bipolar spindle formation. The dysregulated expression of RhoGDIβ may contribute to cancer progression by disrupting these processes.

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