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Article

Flufenamic acid promotes angiogenesis through AMPK activation

  • Authors:
    • Ruiliang Ge
    • Lei Hu
    • Yilin Tai
    • Feng Xue
    • Lei Yuan
    • Gongtian Wei
    • Yi Wang
  • View Affiliations / Copyright

    Affiliations: Division of Hepatic Surgery Ⅱ, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Laboratory of Neural Signal Transduction, State Key Laboratory of Neuroscience, Institute of Neuroscience, Shanghai Institutes of Biological Sciences, Shanghai 200031, P.R. China
  • Pages: 1945-1950
    |
    Published online on: April 10, 2013
       https://doi.org/10.3892/ijo.2013.1891
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Abstract

Angiogenesis plays critical roles in development, tumor growth and metastasis. Flufenamic acid (FFA) is an anti-inflammatory agent known to alter ion fluxes across the plasma membrane. Its role in angiogenesis has not been fully addressed to date. Here, we report that FFA treatment promotes angiogenesis both in vitro and in vivo. Applying FFA for 12 h promoted tube formation of human umbilical vein endothelial cells (HUVECs) without affecting cell proliferation. Three angiogenesis-related genes, VEGF, e-NOS and AAMP, were analyzed by RT-PCR. A significant difference was found between the FFA group and the control; the FFA group had significantly higher mRNA accumulation levels of all the three genes (p<0.05). Moreover, in the chick embryo chorioallantoic membrane (CAM) assay, FFA promoted the formation of macroscopic blood vessels. Finally, western blotting showed that the FFA-treated group had significantly higher phosphorylated AMPK levels, compared with the control (p<0.05). These results suggest that FFA promotes angiogenesis both in vitro and in vivo likely via promoting tube formation through AMPK activation.
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Copy and paste a formatted citation
Spandidos Publications style
Ge R, Hu L, Tai Y, Xue F, Yuan L, Wei G and Wang Y: Flufenamic acid promotes angiogenesis through AMPK activation . Int J Oncol 42: 1945-1950, 2013.
APA
Ge, R., Hu, L., Tai, Y., Xue, F., Yuan, L., Wei, G., & Wang, Y. (2013). Flufenamic acid promotes angiogenesis through AMPK activation . International Journal of Oncology, 42, 1945-1950. https://doi.org/10.3892/ijo.2013.1891
MLA
Ge, R., Hu, L., Tai, Y., Xue, F., Yuan, L., Wei, G., Wang, Y."Flufenamic acid promotes angiogenesis through AMPK activation ". International Journal of Oncology 42.6 (2013): 1945-1950.
Chicago
Ge, R., Hu, L., Tai, Y., Xue, F., Yuan, L., Wei, G., Wang, Y."Flufenamic acid promotes angiogenesis through AMPK activation ". International Journal of Oncology 42, no. 6 (2013): 1945-1950. https://doi.org/10.3892/ijo.2013.1891
Copy and paste a formatted citation
x
Spandidos Publications style
Ge R, Hu L, Tai Y, Xue F, Yuan L, Wei G and Wang Y: Flufenamic acid promotes angiogenesis through AMPK activation . Int J Oncol 42: 1945-1950, 2013.
APA
Ge, R., Hu, L., Tai, Y., Xue, F., Yuan, L., Wei, G., & Wang, Y. (2013). Flufenamic acid promotes angiogenesis through AMPK activation . International Journal of Oncology, 42, 1945-1950. https://doi.org/10.3892/ijo.2013.1891
MLA
Ge, R., Hu, L., Tai, Y., Xue, F., Yuan, L., Wei, G., Wang, Y."Flufenamic acid promotes angiogenesis through AMPK activation ". International Journal of Oncology 42.6 (2013): 1945-1950.
Chicago
Ge, R., Hu, L., Tai, Y., Xue, F., Yuan, L., Wei, G., Wang, Y."Flufenamic acid promotes angiogenesis through AMPK activation ". International Journal of Oncology 42, no. 6 (2013): 1945-1950. https://doi.org/10.3892/ijo.2013.1891
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