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Article

Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2

Corrigendum in: /10.3892/ijo.2016.3695
  • Authors:
    • Li Ma
    • Yueping Liu
    • Cuizhi Geng
    • Xiaowei Qi
    • Jun Jiang
  • View Affiliations / Copyright

    Affiliations: Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China, Department of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P.R. China, Department of Breast Disease Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P.R. China
  • Pages: 1993-2000
    |
    Published online on: April 16, 2013
       https://doi.org/10.3892/ijo.2013.1903
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Abstract

In the present study, we investigated whether estrogen receptor (ER) β affected the proliferation and migration of the human breast cancer cell line MCF-7 through regulation of mitofusin 2 (mfn2). A previous study reported that mfn2 may be regulated by ER through a non-classical pathway; in this pathway, the ER modulates the activities of other transcription factors by stabilizing their binding to DNA and/or recruiting coactivators to the complex. However, the previous study, unlike the study presented here, did not directly explore the interactions between ER and mfn2. Here, RT-PCR and western blot analysis were used to test the expression of mfn2 in MCF-7 cells after exposure to different doses of estradiol (E2). The ability of cells to proliferate and migrate was determined by MTT assay and a monolayer-wounding protocol, respectively. Finally, changes in MCF-7 cell biology after transfection with ERβ or mfn2 expression vectors were investigated, and the role of ERβ in mfn2 expression was also explored. Our results showed that E2 attenuated mfn2 expression in a dose-dependent manner, concomitant with the activation of proliferation and migration of MCF-7 cells. The mfn2 expression vector effectively suppressed E2-induced upregulation of PCNA and migration in MCF-7 cells. ERβ inhibited the E2-induced mfn2 downregulation that accompanied the inhibition of proliferation and migration in MCF-7 cells. Briefly, ERβ may inhibit E2-induced proliferation and migration of MCF-7 cells through regulation of mfn2.
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1 

MacGregor JI and Jordan VC: Basic guide to the mechanisms of antiestrogen action. Pharmacol Rev. 50:151–196. 1998.PubMed/NCBI

2 

Sommer S and Fuqua SA: Estrogen receptor and breast cancer. Semin Cancer Biol. 11:339–352. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Green S, Walter P, Greene G, et al: Cloning of the human oestrogen receptor cDNA. J Steroid Biochem. 24:77–83. 1986. View Article : Google Scholar : PubMed/NCBI

4 

Kuiper GG, Enmark E, Pelto-Huikko M, et al: Cloning of a novel receptor expressed in rat prostate and ovary. Proc Natl Acad Sci USA. 93:5925–5930. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Katzenellenbogen BS, Montano MM, Ediger TR, et al: Estrogen receptors: selective ligands, partners, and distinctive pharmacology. Recent Prog Horm Res. 55:163–193. 2000.PubMed/NCBI

6 

Nilsson S, Makela S, Treuter E, et al: Mechanisms of estrogen action. Physiol Rev. 81:1535–1565. 2001.

7 

Speirs V, Carder PJ, Lane S, et al: Oestrogen receptor β: what it means for patients with breast cancer. Lancet Oncol. 5:174–181. 2004.

8 

Dahlman-Wright K, Cavailles V, Fuqua SA, et al: International Union of Pharmacology. LXIV Estrogen receptors. Pharmacol Rev. 58:773–781. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Harris HA: Estrogen receptor-β: recent lessons from in vivo studies. Mol Endocrinol. 21:1–13. 2007.

10 

Chen KH, Guo X, Ma D, et al: Dysregulation of HSG triggers vascular proliferative disorders. Nat Cell Biol. 6:872–883. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Neuspiel M, Zunino R, Gangaraju S, et al: Activated mitofusin 2 signals mitochondrial fusion, interferes with Bax activation, and reduces susceptibility to radical induced depolarization. J Biol Chem. 280:25060–25070. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Santel A, Frank S, Gaume B, et al: Mitofusin-1 protein is a generally expressed mediator of mitochondrial fusion in mammalian cells. J Cell Sci. 116:2763–2774. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Sugioka R, Shimizu S and Tsujimoto Y: Fzo1, a protein involved in mitochondrial fusion, inhibits apoptosis. J Biol Chem. 279:52726–52734. 2004. View Article : Google Scholar : PubMed/NCBI

14 

DeNardo DG, Kim HT, Hilsenbeck S, et al: Global gene expression analysis of estrogen receptor transcription factor cross talk in breast cancer: identification of estrogen-induced/activator protein-1-dependent genes. Molecular Endocrinology. 19:362–378. 2005. View Article : Google Scholar

15 

Ma L, Liu YP, Geng CZ, et al: Low-dose epirubicin inhibits ezrin-mediated metastatic behavior of breast cancer cells. Tumori. 97:400–405. 2011.PubMed/NCBI

16 

Ström A, Hartman J, Foster JS, et al: Estrogen receptor beta inhibits 17beta-estradiol-stimulated proliferation of the breast cancer cell line T47D. Proc Natl Acad Sci USA. 101:1566–1571. 2004.PubMed/NCBI

17 

Secreto FJ, Monroe DG, Dutta S, et al: Estrogen receptor alpha/beta isoforms, but not betacx, modulate unique patterns of gene expression and cell proliferation in Hs578T cells. J Cell Biochem. 101:1125–1147. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Chen L, Qiu J, Yang C, et al: Identification of a novel estrogen receptor beta1 binding partner, inhibitor of differentiation-1, and role of ERbeta1 in human breast cancer cells. Cancer Lett. 278:210–219. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Jensen EV, Cheng G, Palmieri C, et al: Estrogen receptors and proliferation markers in primary and recurrent breast cancer. Proc Natl Acad Sci USA. 4:42001.PubMed/NCBI

20 

Speirs V, Malone C, Walton DS, et al: Increased expression of estrogen receptor β mRNA in tamoxifen-resistant breast cancer patients. Cancer Res. 59:5421–5424. 1999.

21 

Kushner PJ, Agard DA, Greene GL, et al: Estrogen receptor pathways to AP-1. J Steroid Biochem Mol Biol. 74:311–317. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Wang W, Dong L, Saville B, et al: Transcriptional activation of E2F1 gene expression by 17β-estradiol in MCF-7 cells is regulated by NF-Y-Sp1/estrogen receptor interactions. Mol Endocrinol. 13:1373–1387. 1999.

23 

Safe S: Transcriptional activation of genes by 17β-estradiol through estrogen receptor-Sp1 interactions. Vitam Horm. 62:231–252. 2001.

24 

Ru Lee W, Chen CC, Liu S, et al: 17 beta-estradiol (E2) induces cdc25A gene expression in breast cancer cells by genomic and non-genomic pathways. J Cell Biochem. 99:209–220. 2006.PubMed/NCBI

25 

Yoshioka H, Hiromori Y, Aoki A, et al: Possible aryl hydrocarbon receptor-independent pathway of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-induced antiproliferative response in human breast cancer cells. Toxicol Lett. 211:257–265. 2012. View Article : Google Scholar

26 

Singh KP, Treas J, Tyagi T, et al: DNA demethylation by 5-aza-2-deoxycytidine treatment abrogates 17 beta-estradiol-induced cell growth and restores expression of DNA repair genes in human breast cancer cells. Cancer Lett. 316:62–69. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Wang W, Zhou D, Wei J, et al: Hepatitis B virus X protein inhibits p53-mediated upregulation of mitofusin-2 in hepatocellular carcinoma cells. Biochem Biophys Res Commun. 421:355–360. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Jin B, Fu G, Pan H, et al: Anti-tumour efficacy of mitofusin-2 in urinary bladder carcinoma. Med Oncol. 28(Suppl 1): S373–S380. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Rehman J, Zhang HJ, Toth PT, et al: Inhibition of mitochondrial fission prevents cell cycle progression in lung cancer. FASEB J. 26:2175–2186. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Dotzlaw H, Leygue E, Watson PH, et al: Expression of estrogen receptor-β in human breast tumors. J Clin Endocrinol Metab. 82:2371–2374. 1997.

31 

Fuqua SA, Schiff R, Parra I, et al: Estrogen receptor β protein in human breast cancer: correlation with clinical tumor parameters. Cancer Res. 63:2434–2439. 2003.

32 

Pettersson K, Delaunay F and Gustafsson JA: Estrogen receptor β acts as a dominant regulator of estrogen signaling. Oncogene. 19:4970–4978. 2000.

33 

Matthews J, Wihlen B, Tujague M, et al: Estrogen receptor (ER) β modulates ER α-mediated transcriptional activation by altering the recruitment of c-Fos and c-Jun to estrogen-responsive promoters. Mol Endocrinol. 20:534–543. 2006.

34 

Chang EC, Frasor J, Komm B, et al: Impact of estrogen receptor β on gene networks regulated by estrogen receptor α in breast cancer cells. Endocrinology. 147:4831–4842. 2006.

35 

Lin CY, Strom A, Li Kong S, et al: Inhibitory effects of estrogen receptor beta on specific hormone-responsive gene expression and association with disease outcome in primary breast cancer. Breast Cancer Res. 9:R252007. View Article : Google Scholar : PubMed/NCBI

36 

Williams C, Edvardsson K, Lewandowski SA, et al: A genome-wide study of repressive effects of estrogen receptor beta on estrogen receptor alpha signaling in breast cancer cells. Oncogene. 27:1019–1032. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Paruthiyil S, Parmar H, Kerekatte V, et al: Estrogen receptor β inhibits human breast cancer cell proliferation and tumor formation by causing a G2 cell cycle arrest. Cancer Res. 64:423–428. 2004.

38 

Behrens D, Gill JH and Fichtner I: Loss of tumourigenicity of stably ER β-transfected MCF-7 breast cancer cells. Mol Cell Endocrinol. 274:19–29. 2007.PubMed/NCBI

39 

Nadal-Serrano M, Sastre-Serra J, Pons DG, et al: The ERalpha/ERbeta ratio determines oxidative stress in breast cancer cell lines in response to 17beta-estradiol. J Cell Biochem. 113:3178–3185. 2012. View Article : Google Scholar

40 

Sastre-Serra J, Nadal-Serrano M, Pons DG, et al: The effects of 17β-estradiol on mitochondrial biogenesis and function in breast cancer cell lines are dependent on the ERα/ERβ ratio. Cell Physiol Biochem. 29:261–268. 2012.

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Copy and paste a formatted citation
Spandidos Publications style
Ma L, Liu Y, Geng C, Qi X and Jiang J: Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2 Corrigendum in /10.3892/ijo.2016.3695. Int J Oncol 42: 1993-2000, 2013.
APA
Ma, L., Liu, Y., Geng, C., Qi, X., & Jiang, J. (2013). Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2 Corrigendum in /10.3892/ijo.2016.3695. International Journal of Oncology, 42, 1993-2000. https://doi.org/10.3892/ijo.2013.1903
MLA
Ma, L., Liu, Y., Geng, C., Qi, X., Jiang, J."Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2 Corrigendum in /10.3892/ijo.2016.3695". International Journal of Oncology 42.6 (2013): 1993-2000.
Chicago
Ma, L., Liu, Y., Geng, C., Qi, X., Jiang, J."Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2 Corrigendum in /10.3892/ijo.2016.3695". International Journal of Oncology 42, no. 6 (2013): 1993-2000. https://doi.org/10.3892/ijo.2013.1903
Copy and paste a formatted citation
x
Spandidos Publications style
Ma L, Liu Y, Geng C, Qi X and Jiang J: Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2 Corrigendum in /10.3892/ijo.2016.3695. Int J Oncol 42: 1993-2000, 2013.
APA
Ma, L., Liu, Y., Geng, C., Qi, X., & Jiang, J. (2013). Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2 Corrigendum in /10.3892/ijo.2016.3695. International Journal of Oncology, 42, 1993-2000. https://doi.org/10.3892/ijo.2013.1903
MLA
Ma, L., Liu, Y., Geng, C., Qi, X., Jiang, J."Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2 Corrigendum in /10.3892/ijo.2016.3695". International Journal of Oncology 42.6 (2013): 1993-2000.
Chicago
Ma, L., Liu, Y., Geng, C., Qi, X., Jiang, J."Estrogen receptor β inhibits estradiol-induced proliferation and migration of MCF-7 cells through regulation of mitofusin 2 Corrigendum in /10.3892/ijo.2016.3695". International Journal of Oncology 42, no. 6 (2013): 1993-2000. https://doi.org/10.3892/ijo.2013.1903
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