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October 2013 Volume 43 Issue 4

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Article

Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation

  • Authors:
    • Waraporn Malilas
    • Sang Seok Koh
    • Seokho Kim
    • Ratakorn Srisuttee
    • Il-Rae Cho
    • Jeong Moon
    • Hwa-Seung Yoo
    • Sangtaek Oh
    • Randal N. Johnston
    • Young-Hwa Chung
  • View Affiliations / Copyright

    Affiliations: WCU, Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea, Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, and Department of Functional Genomics, University of Science and Technology, Daejeon 305-333, Republic of Korea, East-West Cancer Center, College of Oriental Medicine, Daejeon University, Daejeon 300-716, Republic of Korea, Department of Advanced Fermentation Fusion Science and Technology, Kookmin University, Seoul 136-702, Republic of Korea, Department of Biochemistry and Molecular Biology, University of Calgary, Calgary T2N4N1, Alberta, Canada
  • Pages: 1111-1116
    |
    Published online on: August 5, 2013
       https://doi.org/10.3892/ijo.2013.2049
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Abstract

Cancer upregulated gene (CUG) 2, as a novel oncogene, has been predominantly detected in various cancer tissues, such as ovary, liver, lung and colon. We recently showed that CUG2 elevates STAT1 activity, leading to resistance to infection by oncolytic vesicular stomatitis virus. To investigate a possible role for CUG2-induced activation of STAT1 in oncogenesis, we first established a colon cancer cell line stably expressing CUG2 (Colon26L5-CUG2). Colon26L5-CUG2 exhibited higher levels not only in phosphorylation of STAT1, but also phosphorylation of Jak1/Tyk2 compared to that of the control (Colon26L5-Vec) cell line. Inhibition of Akt or ERK activity reduced phosphorylation of STAT1 in Colon26L5-CUG2 cells whereas inhibition of p38 MAPK did not significantly decrease levels of STAT1 phosphorylation, indicating that cell proliferation signals may be involved in CUG2-mediated activation of STAT1. Suppression of STAT1 expression diminished cell migration and wound healing compared to the control cells. In addition, since CUG2 expression conferred resistance to DNA damage caused by doxorubicin treatment, we investigated whether STAT1 is involved in resistance to doxorubicin-induced cell death. We found that STAT1 was not activated in Colon26L5-Vec cells while phosphorylated STAT1 was maintained in Colon26L5-CUG2 cells during doxorubicin treatment. Furthermore, suppression of STAT1 expression sensitized Colon26L5-CUG2 cells to doxorubicin-induced apoptosis whereas the control cells exhibited resistance to doxorubicin. Taken together, our results suggest that CUG2 enhances metastasis and drug resistance through STAT1 activation, which eventually contributes to tumor progression.
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Copy and paste a formatted citation
Spandidos Publications style
Malilas W, Koh SS, Kim S, Srisuttee R, Cho I, Moon J, Yoo H, Oh S, Johnston RN, Chung Y, Chung Y, et al: Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation. Int J Oncol 43: 1111-1116, 2013.
APA
Malilas, W., Koh, S.S., Kim, S., Srisuttee, R., Cho, I., Moon, J. ... Chung, Y. (2013). Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation. International Journal of Oncology, 43, 1111-1116. https://doi.org/10.3892/ijo.2013.2049
MLA
Malilas, W., Koh, S. S., Kim, S., Srisuttee, R., Cho, I., Moon, J., Yoo, H., Oh, S., Johnston, R. N., Chung, Y."Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation". International Journal of Oncology 43.4 (2013): 1111-1116.
Chicago
Malilas, W., Koh, S. S., Kim, S., Srisuttee, R., Cho, I., Moon, J., Yoo, H., Oh, S., Johnston, R. N., Chung, Y."Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation". International Journal of Oncology 43, no. 4 (2013): 1111-1116. https://doi.org/10.3892/ijo.2013.2049
Copy and paste a formatted citation
x
Spandidos Publications style
Malilas W, Koh SS, Kim S, Srisuttee R, Cho I, Moon J, Yoo H, Oh S, Johnston RN, Chung Y, Chung Y, et al: Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation. Int J Oncol 43: 1111-1116, 2013.
APA
Malilas, W., Koh, S.S., Kim, S., Srisuttee, R., Cho, I., Moon, J. ... Chung, Y. (2013). Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation. International Journal of Oncology, 43, 1111-1116. https://doi.org/10.3892/ijo.2013.2049
MLA
Malilas, W., Koh, S. S., Kim, S., Srisuttee, R., Cho, I., Moon, J., Yoo, H., Oh, S., Johnston, R. N., Chung, Y."Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation". International Journal of Oncology 43.4 (2013): 1111-1116.
Chicago
Malilas, W., Koh, S. S., Kim, S., Srisuttee, R., Cho, I., Moon, J., Yoo, H., Oh, S., Johnston, R. N., Chung, Y."Cancer upregulated gene 2, a novel oncogene, enhances migration and drug resistance of colon cancer cells via STAT1 activation". International Journal of Oncology 43, no. 4 (2013): 1111-1116. https://doi.org/10.3892/ijo.2013.2049
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