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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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2014-January Volume 44 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas

  • Authors:
    • Gabriela Schneider
    • Mark J. Bowser
    • Dong-Myung Shin
    • Frederic G. Barr
    • Mariusz Z. Ratajczak
  • View Affiliations / Copyright

    Affiliations: Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA, Laboratory of Pathology, National Cancer Institute, Bethesda, MD, USA
  • Pages: 295-300
    |
    Published online on: October 29, 2013
       https://doi.org/10.3892/ijo.2013.2153
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Abstract

Parental imprinting of differentially methylated regions (DMRs) contributes to appropriate expression of several developmentally important genes from paternally or maternally derived chromosomes. Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma in children and is associated with altered expression of certain parentally imprinted genes. As previously reported, RMS cells display loss of imprinting (LOI) of the DMR at the IGF2-H19 locus, resulting in insulin-like growth factor 2 (IGF2) transcription from both paternally and maternally inherited chromosomes, and overall IGF2 overexpression. As the DLK1-GTL2 locus is structurally similar to the IGF2-H19 locus, the status of parental imprinting of the DLK1-GTL2 locus was studied in RMS. We observed that while both embryonal and alveolar rhabdomyosarcomas (ERMS and ARMS, respectively) show LOI of the DMR at the IGF2-H19 locus, imprinting of the DMR at the DLK1-GTL2 locus varies in association with the histological subtype of RMS. We found that, while ERMS tumors consistently show LOI of the DMR at the DLK1-GTL2 locus, ARMS tumors have erasure of imprinting (EOI) at this locus. These changes in imprinting status of the DLK1-GTL2 locus result in a higher GTL2/DLK1 mRNA ratio in ARMS as compared to ERMS. This difference in imprinting elucidates a novel genetic difference between these two RMS subtypes and may provide a potential diagnostic tool to distinguish between these subtypes.
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1. 

Davis E, Jensen CH, Schroder HD, et al: Ectopic expression of DLK1 protein in skeletal muscle of padumnal heterozygotes causes the callipyge phenotype. Curr Biol. 14:1858–1862. 2004. View Article : Google Scholar : PubMed/NCBI

2. 

Davicioni E, Anderson MJ, Finckenstein FG, et al: Molecular classification of rhabdomyosarcoma - genotypic and phenotypic determinants of diagnosis: a report from the Children’s Oncology Group. Am J Pathol. 174:550–564. 2009.PubMed/NCBI

3. 

Hettmer S and Wagers AJ: Muscling in: Uncovering the origins of rhabdomyosarcoma. Nat Med. 16:171–173. 2010. View Article : Google Scholar : PubMed/NCBI

4. 

Chung WY, Yuan L, Feng L, Hensle T and Tycko B: Chromosome 11p15.5 regional imprinting: comparative analysis of KIP2 and H19 in human tissues and Wilms’ tumors. Hum Mol Genet. 5:1101–1108. 1996.PubMed/NCBI

5. 

Casola S, Pedone PV, Cavazzana AO, et al: Expression and parental imprinting of the H19 gene in human rhabdomyosarcoma. Oncogene. 14:1503–1510. 1997. View Article : Google Scholar : PubMed/NCBI

6. 

Ishida M and Moore GE: The role of imprinted genes in humans. Mol Aspects Med. 34:826–840. 2013. View Article : Google Scholar : PubMed/NCBI

7. 

Reik W and Walter J: Genomic imprinting: parental influence on the genome. Nat Rev Genet. 2:21–32. 2001. View Article : Google Scholar : PubMed/NCBI

8. 

Pannetier M and Feil R: Epigenetic stability of embryonic stem cells and developmental potential. Trends Biotechnol. 25:556–562. 2007. View Article : Google Scholar : PubMed/NCBI

9. 

Delaval K and Feil R: Epigenetic regulation of mammalian genomic imprinting. Curr Opin Genet Dev. 14:188–195. 2004. View Article : Google Scholar : PubMed/NCBI

10. 

Geuns E, De Temmerman N, Hilven P, Van Steirteghem A, Liebaers I and De Rycke M: Methylation analysis of the intergenic differentially methylated region of DLK1-GTL2 in human. Eur J Hum Genet. 15:352–361. 2007. View Article : Google Scholar : PubMed/NCBI

11. 

Sasaki H, Ishihara K and Kato R: Mechanisms of Igf2/H19 imprinting: DNA methylation, chromatin and long-distance gene regulation. J Biochem. 127:711–715. 2000. View Article : Google Scholar : PubMed/NCBI

12. 

Waddell JN, Zhang P, Wen Y, et al: Dlk1 is necessary for proper skeletal muscle development and regeneration. PLoS One. 5:e150552010. View Article : Google Scholar : PubMed/NCBI

13. 

Markljung E, Jiang L, Jaffe JD, et al: ZBED6, a novel transcription factor derived from a domesticated DNA transposon regulates IGF2 expression and muscle growth. PLoS Biol. 7:e10002562009. View Article : Google Scholar : PubMed/NCBI

14. 

Duan F, Smith LM, Gustafson DM, et al: Genomic and clinical analysis of fusion gene amplification in rhabdomyosarcoma: a report from the Children’s Oncology Group. Genes Chromosomes Cancer. 51:662–674. 2012.

15. 

Kerjean A, Dupont JM, Vasseur C, et al: Establishment of the paternal methylation imprint of the human H19 and MEST/PEG1 genes during spermatogenesis. Hum Mol Genet. 9:2183–2187. 2000. View Article : Google Scholar : PubMed/NCBI

16. 

Higashimoto K, Urano T, Sugiura K, et al: Loss of CpG methylation is strongly correlated with loss of histone H3 lysine 9 methylation at DMR-LIT1 in patients with Beckwith-Wiedemann syndrome. Am J Hum Genet. 73:948–956. 2003. View Article : Google Scholar : PubMed/NCBI

17. 

Kobayashi H, Sato A, Otsu E, et al: Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Hum Mol Genet. 16:2542–2551. 2007. View Article : Google Scholar : PubMed/NCBI

18. 

Carr IM, Valleley EM, Cordery SF, Markham AF and Bonthron DT: Sequence analysis and editing for bisulphite genomic sequencing projects. Nucleic Acids Res. 35:e792007. View Article : Google Scholar : PubMed/NCBI

19. 

Kagami M, Sekita Y, Nishimura G, et al: Deletions and epimutations affecting the human 14q32.2 imprinted region in individuals with paternal and maternal upd(14)-like phenotypes. Nat Genet. 40:237–242. 2008. View Article : Google Scholar : PubMed/NCBI

20. 

Ogata T, Kagami M and Ferguson-Smith AC: Molecular mechanisms regulating phenotypic outcome in paternal and maternal uniparental disomy for chromosome 14. Epigenetics. 3:181–187. 2008. View Article : Google Scholar : PubMed/NCBI

21. 

Benetatos L, Hatzimichael E, Londin E, et al: The microRNAs within the DLK1-DIO3 genomic region: involvement in disease pathogenesis. Cell Mol Life Sci. 70:795–814. 2013. View Article : Google Scholar : PubMed/NCBI

22. 

Li L, Forman SJ and Bhatia R: Expression of DLK1 in hematopoietic cells results in inhibition of differentiation and proliferation. Oncogene. 24:4472–4476. 2005. View Article : Google Scholar : PubMed/NCBI

23. 

Liu L, Luo GZ, Yang W, et al: Activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells. J Biol Chem. 285:19483–19490. 2010. View Article : Google Scholar : PubMed/NCBI

24. 

Zhou Y, Zhang X and Klibanski A: MEG3 noncoding RNA: a tumor suppressor. J Mol Endocrinol. 48:R45–R53. 2012. View Article : Google Scholar : PubMed/NCBI

25. 

Khoury H, Suarez-Saiz F, Wu S and Minden MD: An upstream insulator regulates DLK1 imprinting in AML. Blood. 115:2260–2263. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Schneider G, Bowser MJ, Shin D, Barr FG and Ratajczak MZ: The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas. Int J Oncol 44: 295-300, 2014.
APA
Schneider, G., Bowser, M.J., Shin, D., Barr, F.G., & Ratajczak, M.Z. (2014). The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas. International Journal of Oncology, 44, 295-300. https://doi.org/10.3892/ijo.2013.2153
MLA
Schneider, G., Bowser, M. J., Shin, D., Barr, F. G., Ratajczak, M. Z."The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas". International Journal of Oncology 44.1 (2014): 295-300.
Chicago
Schneider, G., Bowser, M. J., Shin, D., Barr, F. G., Ratajczak, M. Z."The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas". International Journal of Oncology 44, no. 1 (2014): 295-300. https://doi.org/10.3892/ijo.2013.2153
Copy and paste a formatted citation
x
Spandidos Publications style
Schneider G, Bowser MJ, Shin D, Barr FG and Ratajczak MZ: The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas. Int J Oncol 44: 295-300, 2014.
APA
Schneider, G., Bowser, M.J., Shin, D., Barr, F.G., & Ratajczak, M.Z. (2014). The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas. International Journal of Oncology, 44, 295-300. https://doi.org/10.3892/ijo.2013.2153
MLA
Schneider, G., Bowser, M. J., Shin, D., Barr, F. G., Ratajczak, M. Z."The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas". International Journal of Oncology 44.1 (2014): 295-300.
Chicago
Schneider, G., Bowser, M. J., Shin, D., Barr, F. G., Ratajczak, M. Z."The paternally imprinted DLK1-GTL2 locus is differentially methylated in embryonal and alveolar rhabdomyosarcomas". International Journal of Oncology 44, no. 1 (2014): 295-300. https://doi.org/10.3892/ijo.2013.2153
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