MicroRNA-126 functions as a tumor suppressor in colorectal cancer cells by targeting CXCR4 via the AKT and ERK1/2 signaling pathways

  • Authors:
    • Yaling Liu
    • Yu Zhou
    • Xiao Feng
    • Ping An
    • Xiaojing Quan
    • Hao Wang
    • Shicai Ye
    • Caiyuan Yu
    • Yanting He
    • Hesheng Luo
  • View Affiliations

  • Published online on: November 5, 2013     https://doi.org/10.3892/ijo.2013.2168
  • Pages: 203-210
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Abstract

Recent evidence shows that altered microRNA-126 (miR-126) expression is implicated in the progression of colorectal cancer (CRC). However, the precise roles and mechanisms of miR-126 in CRC remain unclear. The aim of this study was to investigate the roles of miR-126 in CRC cells and to elucidate miR-126-mediated mechanisms in CRC cells. First, miR-126 expression was analyzed using qRT-PCR in 4 human CRC cell lines (SW480, SW620, HT-29 and HCT-116). Furthermore, the biological properties of miR-126 in CRC cells in vitro were examined by applying Cell Counting Kit 8, cell cycle, cell apoptosis and transwell assays. The mechanisms and pathways of miR-126-mediated in CRC cells were detected by using qRT-PCR, western blotting and luciferase reporter assay. We found that miR-126 overexpression inhibited cell proliferation, migration and invasion, and induced cell arrest in the G0/G1 phase of CRC cells, suggesting that miR-126 functions as a tumor suppressor in CRC cells. Furthermore, we identified the CXC chemokine receptor 4 (CXCR4) as a target of miR-126, and showed that it was negatively regulated by miR-126. We demonstrated that miR-126-mediated tumor suppression might be partly dependent on AKT and ERK1/2 signaling pathways. In conclusion, our data revealed that miR-126 functions as a tumor suppressor in CRC cells by regulating CXCR4 expression via the AKT and ERK1/2 signaling pathways and might be a novel target for therapeutic strategies in CRC.
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2014-January
Volume 44 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Liu Y, Zhou Y, Feng X, An P, Quan X, Wang H, Ye S, Yu C, He Y, Luo H, Luo H, et al: MicroRNA-126 functions as a tumor suppressor in colorectal cancer cells by targeting CXCR4 via the AKT and ERK1/2 signaling pathways. Int J Oncol 44: 203-210, 2014
APA
Liu, Y., Zhou, Y., Feng, X., An, P., Quan, X., Wang, H. ... Luo, H. (2014). MicroRNA-126 functions as a tumor suppressor in colorectal cancer cells by targeting CXCR4 via the AKT and ERK1/2 signaling pathways. International Journal of Oncology, 44, 203-210. https://doi.org/10.3892/ijo.2013.2168
MLA
Liu, Y., Zhou, Y., Feng, X., An, P., Quan, X., Wang, H., Ye, S., Yu, C., He, Y., Luo, H."MicroRNA-126 functions as a tumor suppressor in colorectal cancer cells by targeting CXCR4 via the AKT and ERK1/2 signaling pathways". International Journal of Oncology 44.1 (2014): 203-210.
Chicago
Liu, Y., Zhou, Y., Feng, X., An, P., Quan, X., Wang, H., Ye, S., Yu, C., He, Y., Luo, H."MicroRNA-126 functions as a tumor suppressor in colorectal cancer cells by targeting CXCR4 via the AKT and ERK1/2 signaling pathways". International Journal of Oncology 44, no. 1 (2014): 203-210. https://doi.org/10.3892/ijo.2013.2168