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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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June-2014 Volume 44 Issue 6

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression

  • Authors:
    • Jia-Jia Zhou
    • Xiao-Geng Deng
    • Xiao-Yu He
    • Yu Zhou
    • Min Yu
    • Wen-Chao Gao
    • Bing Zeng
    • Quan-Bo Zhou
    • Zhi-Hua Li
    • Ru-Fu Chen
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, P.R. China, Laboratory of Biomechanics and Physiology, Guangdong Provincial Institute of Sports Science, Guangzhou, Guangdong, P.R. China, Department of Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, P.R. China
  • Pages: 2034-2040
    |
    Published online on: March 19, 2014
       https://doi.org/10.3892/ijo.2014.2347
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Abstract

Multidrug resistance (MDR) is one of the major reasons for the failure of liver cancer chemotherapy, and its suppression may increase the efficacy of chemotherapy. NANOG plays a key role in the regulation of embryonic stem cell self-renewal and pluripotency. Recent studies reported that NANOG was abnormally expressed in several types of tumors, indicating that NANOG is related to tumor development. However, the correlation between NANOG and liver cancer chemoresistance remains uncertain. In this study, RNA interfere technology was employed to knock down NANOG expression in HepG2 human liver cancer cells. We found that the knockdown of NANOG expression in NANOG siRNA-transfected HepG2 cells resulted in decreased colony formation rate and cell migration compared to control HepG2 cells. In addition, HepG2 cells were treated with doxorubicin to evaluate the chemosensitivity to doxorubicin. We found that the doxorubicin sensitivity of HepG2 cells was increased with downregulation of NANOG expression. The expression of MDR1 at both mRNA and protein levels was decreased in HepG2 cells when NANOG was knocked down. These findings suggest that the knockdown of NANOG in HepG2 human cells resulted in decreased MDR1 expression and increased doxorubicin sensitivity, and NANOG could be used as a novel potential therapeutic target to reverse multidrug resistance of liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Deng X, He X, Zhou Y, Yu M, Gao W, Zeng B, Zhou Q, Li Z, Chen R, Chen R, et al: Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression. Int J Oncol 44: 2034-2040, 2014.
APA
Zhou, J., Deng, X., He, X., Zhou, Y., Yu, M., Gao, W. ... Chen, R. (2014). Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression. International Journal of Oncology, 44, 2034-2040. https://doi.org/10.3892/ijo.2014.2347
MLA
Zhou, J., Deng, X., He, X., Zhou, Y., Yu, M., Gao, W., Zeng, B., Zhou, Q., Li, Z., Chen, R."Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression". International Journal of Oncology 44.6 (2014): 2034-2040.
Chicago
Zhou, J., Deng, X., He, X., Zhou, Y., Yu, M., Gao, W., Zeng, B., Zhou, Q., Li, Z., Chen, R."Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression". International Journal of Oncology 44, no. 6 (2014): 2034-2040. https://doi.org/10.3892/ijo.2014.2347
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Deng X, He X, Zhou Y, Yu M, Gao W, Zeng B, Zhou Q, Li Z, Chen R, Chen R, et al: Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression. Int J Oncol 44: 2034-2040, 2014.
APA
Zhou, J., Deng, X., He, X., Zhou, Y., Yu, M., Gao, W. ... Chen, R. (2014). Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression. International Journal of Oncology, 44, 2034-2040. https://doi.org/10.3892/ijo.2014.2347
MLA
Zhou, J., Deng, X., He, X., Zhou, Y., Yu, M., Gao, W., Zeng, B., Zhou, Q., Li, Z., Chen, R."Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression". International Journal of Oncology 44.6 (2014): 2034-2040.
Chicago
Zhou, J., Deng, X., He, X., Zhou, Y., Yu, M., Gao, W., Zeng, B., Zhou, Q., Li, Z., Chen, R."Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression". International Journal of Oncology 44, no. 6 (2014): 2034-2040. https://doi.org/10.3892/ijo.2014.2347
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