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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2014 Volume 45 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma

  • Authors:
    • Jia Li
    • Jian-Zhi Gao
    • Jing-Li Du
    • Zhong-Xi Huang
    • Li-Xin Wei
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Chinese PLA General Hospital, Beijing 100853, P.R. China, Cancer Institute, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
  • Pages: 1547-1555
    |
    Published online on: July 24, 2014
       https://doi.org/10.3892/ijo.2014.2559
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Abstract

Cell division cycle 20 (CDC20) encodes a regulatory protein interacting with the anaphase-promoting complex/cyclosome (APC/C) in the cell cycle and plays important roles in tumorigenesis and progression of multiple tumors. The present study aimed to investigate the clinical significance of CDC20 in hepatocellular carcinoma (HCC) and the role of CDC20 in the progression of HCC. By bioinformatics analysis, CDC20 was found to be the major node in HCC molecular interaction networks. Quantitative PCR and western blot analyses were applied to examine CDC20 expression in 16 paired primary HCC tissues. Immunohistochemistry (IHC) was performed to examine CDC20 protein expression in 132 matched paraffin-embedded HCC tissues and to analyze the relationship between CDC20 staining and clinical characteristics. Small interfering RNA (siRNA) targeting CDC20 was synthesized and transfected into HepG2 cells to investigate the role of CDC20 in cell growth and the cell cycle. Results show that CDC20 expression was upregulated in HCC tissues compared to adjacent non-tumor liver tissues. In the 132 matched HCC tissues, high expression levels of CDC20 were detected in 68.18% HCC samples, and overexpression of CDC20 was positively correlated with gender (P=0.013), tumor differentiation (P=0.000), TNM stage (P=0.012), P53 and Ki-67 expression (P=0.023 and P=0.007, respectively). Cells transfected with CDC20 siRNA showed a decrease in cell proliferation and increase in the number of cells in G2/M-phase. In conclusion, increased expression of CDC20 was demonstrated to be associated with the development and progression of HCC, and may be regarded as a promising therapeutic target for HCC.
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1 

Parkin DM: Global cancer statistics in the year 2000. Lancet Oncol. 2:533–543. 2001.PubMed/NCBI

2 

El-Serag HB and Rudolph KL: Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology. 132:2557–2576. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

4 

Severi T, van Malenstein H, Verslype C and van Pelt JF: Tumor initiation and progression in hepatocellular carcinoma: risk factors, classification, and therapeutic targets. Acta Pharmacol Sin. 31:1409–1420. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Michielsen P and Ho E: Viral hepatitis B and hepatocellular carcinoma. Acta Gastroenterol Belg. 74:4–8. 2011.

6 

McGivern DR and Lemon SM: Virus-specific mechanisms of carcinogenesis in hepatitis C virus associated liver cancer. Oncogene. 30:1969–1983. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Fung TK and Poon RY: A roller coaster ride with the mitotic cyclins. Semin Cell Dev Biol. 16:335–342. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Weinstein J, Jacobsen FW, Hsu-Chen J, Wu T and Baum LG: A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4. Mol Cell Biol. 14:3350–3363. 1994.

9 

Peters JM: Cell biology: the checkpoint brake relieved. Nature. 446:868–869. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Fang G, Yu H and Kirschner MW: Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1. Mol Cell. 2:163–171. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Cho HJ, Lee EH, Han SH, et al: Degradation of human RAP80 is cell cycle regulated by Cdc20 and Cdh1 ubiquitin ligases. Mol Cancer Res. 10:615–625. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Kidokoro T, Tanikawa C, Furukawa Y, Katagiri T, Nakamura Y and Matsuda K: CDC20, a potential cancer therapeutic target, is negatively regulated by p53. Oncogene. 27:1562–1571. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Chang DZ, Ma Y, Ji B, et al: Increased CDC20 expression is associated with pancreatic ductal adenocarcinoma differentiation and progression. J Hematol Oncol. 5:152012. View Article : Google Scholar : PubMed/NCBI

14 

Kim JM, Sohn HY, Yoon SY, et al: Identification of gastric cancer-related genes using a cDNA microarray containing novel expressed sequence tags expressed in gastric cancer cells. Clin Cancer Res. 11:473–482. 2005.PubMed/NCBI

15 

Espinosa AM, Alfaro A, Roman-Basaure E, et al: Mitosis is a source of potential markers for screening and survival and therapeutic targets in cervical cancer. PloS One. 8:e559752013.PubMed/NCBI

16 

Ouellet V, Guyot MC, Le Page C, et al: Tissue array analysis of expression microarray candidates identifies markers associated with tumor grade and outcome in serous epithelial ovarian cancer. Int J Cancer. 119:599–607. 2006. View Article : Google Scholar

17 

Roessler S, Jia HL, Budhu A, et al: A unique metastasis gene signature enables prediction of tumor relapse in early-stage hepatocellular carcinoma patients. Cancer Res. 70:10202–10212. 2010. View Article : Google Scholar

18 

Irizarry RA, Hobbs B, Collin F, et al: Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 4:249–264. 2003. View Article : Google Scholar

19 

Dai M, Wang P, Boyd AD, et al: Evolving gene/transcript definitions significantly alter the interpretation of GeneChip data. Nucleic Acids Res. 33:e1752005. View Article : Google Scholar : PubMed/NCBI

20 

Tusher VG, Tibshirani R and Chu G: Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA. 98:5116–5121. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Huang ZX, Tian HY, Hu ZF, Zhou YB, Zhao J and Yao KT: GenCLiP: a software program for clustering gene lists by literature profiling and constructing gene co-occurrence networks related to custom keywords. BMC Bioinformatics. 9:3082008. View Article : Google Scholar : PubMed/NCBI

22 

Adhikary S and Eilers M: Transcriptional regulation and transformation by Myc proteins. Nat Rev Mol Cell Biol. 6:635–645. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Detre S, Saclani Jotti G and Dowsett M: A ‘quickscore’ method for immunohistochemical semiquantitation: validation for oestrogen receptor in breast carcinomas. J Clin Pathol. 48:876–878. 1995.

24 

Früh MPM: EGFR IHC score for selection of cetuximab treatment: Ready for clinical practice? Transl Lung Cancer Res. 1:145–146. 2012.

25 

Hwang LH, Lau LF, Smith DL, et al: Budding yeast Cdc20: a target of the spindle checkpoint. Science. 279:1041–1044. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Kops GJ, Weaver BA and Cleveland DW: On the road to cancer: aneuploidy and the mitotic checkpoint. Nat Rev Cancer. 5:773–785. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Mondal G, Sengupta S, Panda CK, Gollin SM, Saunders WS and Roychoudhury S: Overexpression of Cdc20 leads to impairment of the spindle assembly checkpoint and aneuploidization in oral cancer. Carcinogenesis. 28:81–92. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Moura IM, Delgado ML, Silva PM, et al: High CDC20 expression is associated with poor prognosis in oral squamous cell carcinoma. J Oral Pathol Med. 43:225–231. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Wu WJ, Hu KS, Wang DS, et al: CDC20 overexpression predicts a poor prognosis for patients with colorectal cancer. J Transl Med. 11:1422013. View Article : Google Scholar : PubMed/NCBI

30 

Yeong FM, Lim HH, Padmashree CG and Surana U: Exit from mitosis in budding yeast: biphasic inactivation of the Cdc28-Clb2 mitotic kinase and the role of Cdc20. Mol Cell. 5:501–511. 2000. View Article : Google Scholar : PubMed/NCBI

31 

Huang HC, Shi J, Orth JD and Mitchison TJ: Evidence that mitotic exit is a better cancer therapeutic target than spindle assembly. Cancer Cell. 16:347–358. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Wang Z, Wan L, Zhong J, et al: Cdc20: a potential novel therapeutic target for cancer treatment. Curr Pharm Des. 19:3210–3214. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Bunz F, Dutriaux A, Lengauer C, et al: Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science. 282:1497–1501. 1998. View Article : Google Scholar : PubMed/NCBI

34 

Wells J, Boyd KE, Fry CJ, Bartley SM and Farnham PJ: Target gene specificity of E2F and pocket protein family members in living cells. Mol Cell Biol. 20:5797–5807. 2000. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Li J, Gao J, Du J, Huang Z and Wei L: Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma. Int J Oncol 45: 1547-1555, 2014.
APA
Li, J., Gao, J., Du, J., Huang, Z., & Wei, L. (2014). Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma. International Journal of Oncology, 45, 1547-1555. https://doi.org/10.3892/ijo.2014.2559
MLA
Li, J., Gao, J., Du, J., Huang, Z., Wei, L."Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma". International Journal of Oncology 45.4 (2014): 1547-1555.
Chicago
Li, J., Gao, J., Du, J., Huang, Z., Wei, L."Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma". International Journal of Oncology 45, no. 4 (2014): 1547-1555. https://doi.org/10.3892/ijo.2014.2559
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Gao J, Du J, Huang Z and Wei L: Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma. Int J Oncol 45: 1547-1555, 2014.
APA
Li, J., Gao, J., Du, J., Huang, Z., & Wei, L. (2014). Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma. International Journal of Oncology, 45, 1547-1555. https://doi.org/10.3892/ijo.2014.2559
MLA
Li, J., Gao, J., Du, J., Huang, Z., Wei, L."Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma". International Journal of Oncology 45.4 (2014): 1547-1555.
Chicago
Li, J., Gao, J., Du, J., Huang, Z., Wei, L."Increased CDC20 expression is associated with development and progression of hepatocellular carcinoma". International Journal of Oncology 45, no. 4 (2014): 1547-1555. https://doi.org/10.3892/ijo.2014.2559
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