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Article

Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway

  • Authors:
    • Soon-Jae Kwon
    • Ju-Hye Lee
    • Kwang-Deog Moon
    • Il-Yun Jeong
    • Sung-Tae Yee
    • Mi-Kyung Lee
    • Kwon-Il Seo
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Technology, Kyungpook National University, Daegu 702-701, Republic of Korea, Department of Food and Nutrition, Sunchon National University, Suncheon, Jeonnam 540-742, Republic of Korea, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk 580-185, Republic of Korea, Department of Biology, Sunchon National University, Suncheon, Jeonnam 540-742, Republic of Korea
  • Pages: 1969-1976
    |
    Published online on: August 14, 2014
       https://doi.org/10.3892/ijo.2014.2598
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Abstract

Isoegomaketone (IK) is a major biologically active component of Perilla frutescens. In this study, we investigated the contribution of reactive oxygen species (ROS) to IK-induced apoptosis in human melanoma SK-MEL-2 cells. We found that IK inhibited the proliferation of SK-MEL-2 human melanoma cells in a dose-dependent manner. IK also induced sub-G1 DNA accumulation, formation of apoptotic bodies, nuclear condensation, and a DNA ladder in SK-MEL-2 cells. IK also induced activation of caspase-3 and -9, whereas caspase‑8 was unaffected. Further, N-acetyl-L-cysteine (NAC, ROS scavenger) treatment to SK-MEL-2 cells significantly reduced IK-induced cell death. Pretreatment of NAC to SK-MEL-2 cells followed by 100 µM IK reduced the protein levels of Bax and cytochrome c as well as PARP cleavage, whereas the protein level of Bcl-2 increased. Moreover, IK inhibited the phosphorylation of AKT/mTOR protein and cell proliferation induced by LY294002, a PI3K inhibitor. In conclusion, IK-induced ROS generation regulates cell growth inhibition and it induces apoptosis through caspase‑dependent and -independent pathways via modulation of PI3K/AKT signaling in SK-MEL-2 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Kwon S, Lee J, Moon K, Jeong I, Yee S, Lee M and Seo K: Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway. Int J Oncol 45: 1969-1976, 2014.
APA
Kwon, S., Lee, J., Moon, K., Jeong, I., Yee, S., Lee, M., & Seo, K. (2014). Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway. International Journal of Oncology, 45, 1969-1976. https://doi.org/10.3892/ijo.2014.2598
MLA
Kwon, S., Lee, J., Moon, K., Jeong, I., Yee, S., Lee, M., Seo, K."Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway". International Journal of Oncology 45.5 (2014): 1969-1976.
Chicago
Kwon, S., Lee, J., Moon, K., Jeong, I., Yee, S., Lee, M., Seo, K."Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway". International Journal of Oncology 45, no. 5 (2014): 1969-1976. https://doi.org/10.3892/ijo.2014.2598
Copy and paste a formatted citation
x
Spandidos Publications style
Kwon S, Lee J, Moon K, Jeong I, Yee S, Lee M and Seo K: Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway. Int J Oncol 45: 1969-1976, 2014.
APA
Kwon, S., Lee, J., Moon, K., Jeong, I., Yee, S., Lee, M., & Seo, K. (2014). Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway. International Journal of Oncology, 45, 1969-1976. https://doi.org/10.3892/ijo.2014.2598
MLA
Kwon, S., Lee, J., Moon, K., Jeong, I., Yee, S., Lee, M., Seo, K."Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway". International Journal of Oncology 45.5 (2014): 1969-1976.
Chicago
Kwon, S., Lee, J., Moon, K., Jeong, I., Yee, S., Lee, M., Seo, K."Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway". International Journal of Oncology 45, no. 5 (2014): 1969-1976. https://doi.org/10.3892/ijo.2014.2598
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