Effects of nutrients on matrix metalloproteinases in human T-lymphotropic virus type 1 positive and negative malignant T-lymphocytes Retraction in /10.3892/ijo.2022.5451

Harakeh,Steve; Abou-Khouzam,Raefa; Damanhouri,Ghazi  A.; Al-Hejin,Ahmed; Kumosani,Taha; Niedzwiecki,Aleksandra; Rath,Mathias; Barbour,Elie; Diab-Assaf,Mona; Azar,Rania

doi:10.3892/ijo.2014.2638

Effects of nutrients on matrix metalloproteinases in human T-lymphotropic virus type 1 positive and negative malignant T-lymphocytes

Retraction in: /10.3892/ijo.2022.5451

Authors:
- Steve Harakeh
- Raefa Abou-Khouzam
- Ghazi A. Damanhouri
- Ahmed Al-Hejin
- Taha Kumosani
- Aleksandra Niedzwiecki
- Mathias Rath
- Elie Barbour
- Mona Diab-Assaf
- Rania Azar
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Affiliations: Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Kingdom of Saudi Arabia, Molecular Tumor-genesis and Anticancer Pharmacology, EDST, Lebanese University, Hadath, Lebanon, Department of Hematology, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, Department of Biological Sciences, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, Department of Biochemistry, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, Dr. Rath Research Institute, Santa Clara, CA, USA, Department of Animal and Veterinary Sciences, American University of Beirut (AUB), Beirut 11-0236, Lebanon
Published online on: September 3, 2014 https://doi.org/10.3892/ijo.2014.2638
Pages: 2159-2166

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Abstract

Experimental and clinical studies have revealed the effectiveness of a specific nutrient synergy (SNS) mixture composed of ascorbic acid (AA), lysine, proline, arginine, epigallocatechin gallate (EGCG) and other micronutrients in targeting crucial physiological mechanisms involved in cancer progression and metastasis. HTLV-1 causes adult T-cell leukemia (ATL). The spread and metastases of ATL as well as other tumors has been associated with matrix metalloproteinases, especially the gelatinases MMP-2 and MMP-9. The objective of this study was to investigate whether SNS, AA and EGCG affects the gelatinolytic activity of MMP-2 and its transcriptional and translational levels in HTLV-1-positive and -negative malignant T-cells. The results indicated that SNS and EGCG caused a dose-dependent decline in the activity, transcription and translation of MMP-2 after treatment with SNS and EGCG, while AA was only able to inhibit the activity at maximum doses tested and to some extent, the protein expression levels of MMP-2, without affecting their transcriptional levels. The highest activity was noted in the case of SNS which is likely to be due to a synergistic effect of the different constituents in the formulation. These results point towards the potential integration of SNS in the anti-invasive treatment of ATL and related diseases.

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