Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2

  • Authors:
    • Wei Wang
    • Bin Chen
    • Ruolan Zou
    • Xiuying Tu
    • Songlin Tan
    • Hong Lu
    • Zhaojie Liu
    • Jianjiang Fu
  • View Affiliations

  • Published online on: September 4, 2014     https://doi.org/10.3892/ijo.2014.2643
  • Pages: 1891-1900
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Abstract

Metastasis is the most insidious aspect of breast cancer, but effective strategies to control this malignant process are still lacking. In previous studies, we screened over 200 extracts from plants of genus Chloranthaceae by bioactivity-guided fractionation, and found that Codonolactone (CLT) exhibited potential antimetastatic properties in breast cancer cells. This sesquiterpene lactone was isolated from Chloranthus henryi Hemsl, and is also found in other medical herbs, such as Codonopsis pilosula, Atractylodes macrocephala Koidz and others. Here, we report that CLT inhibited the ability of invasion and migration in metastatic breast cancer cells. Furthermore, CLT exhibited significant suppression on formation of lung metastatic foci of breast cancer in vivo. We next investigated the mechanism of CLT-induced metastasis inhibitory effects in breast cancer cells. A significant inhibition on activity and expression of MMP-9 and MMP-13 was observed. Moreover, data from western blotting, Runx2 transcription factor assay and chromatin immunoprecipitation assay showed that binding ability of Runx2 to sequences of the mmp-13 promoter was inhibited by CLT. Collectively, these findings suggested that the antimetastatic properties of CLT in breast cancer were due to the inhibition of MMPs, which might be associated with a downregulation of Runx2 transcriptional activity.
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November-2014
Volume 45 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wang W, Chen B, Zou R, Tu X, Tan S, Lu H, Liu Z and Fu J: Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2. Int J Oncol 45: 1891-1900, 2014
APA
Wang, W., Chen, B., Zou, R., Tu, X., Tan, S., Lu, H. ... Fu, J. (2014). Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2. International Journal of Oncology, 45, 1891-1900. https://doi.org/10.3892/ijo.2014.2643
MLA
Wang, W., Chen, B., Zou, R., Tu, X., Tan, S., Lu, H., Liu, Z., Fu, J."Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2". International Journal of Oncology 45.5 (2014): 1891-1900.
Chicago
Wang, W., Chen, B., Zou, R., Tu, X., Tan, S., Lu, H., Liu, Z., Fu, J."Codonolactone, a sesquiterpene lactone isolated from Chloranthus henryi Hemsl, inhibits breast cancer cell invasion, migration and metastasis by downregulating the transcriptional activity of Runx2". International Journal of Oncology 45, no. 5 (2014): 1891-1900. https://doi.org/10.3892/ijo.2014.2643