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Article

HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia

  • Authors:
    • Qian Kong
    • Lu-Hong Xu
    • Wei Xu
    • Jian-Pei Fang
    • Hong-Gui Xu
  • View Affiliations / Copyright

    Affiliations: Guangdong Provincial Key Laboratory of Malignant Tumour Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, P.R. China, Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, P.R. China
  • Pages: 161-170
    |
    Published online on: May 4, 2015
       https://doi.org/10.3892/ijo.2015.2985
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Abstract

Acute lymphoblastic leukaemia (ALL) is a common paediatric cancer and is among the most curable cancers. However, the acquisition of drug resistance is a significant obstacle to the achievement of favourable outcomes, and autophagy is regarded as a mechanism that underlies chemoresistance. In this study, RT-qPCR was used to measure the expression of HMGB1 and Beclin1 in bone marrow mononuclear cells. A CCK-8 test was conducted to assess cell viability. Western blot, immunofluorescence and transmission electron microscopic analyses were performed to evaluate the autophagy levels. Immunoprecipitation analysis was performed to detect protein-protein interactions in the autophagy complexes. We found that HMGB1 expression correlated with the clinical status of ALL. In vitro, anticancer agent-induced cytotoxic effects were associated with autophagy-related drug resistance, and these effects were ameliorated by FIP200 depletion or the application of autophagy inhibitors. Moreover, the Ulk1‑Atg13-FIP200 complex, which promotes HMGB1 trafficking, acted upstream of the HMGB1-Beclin1 and PI3KC3-Beclin1 complexes and played a critical role in autophagy. Targeting the transformation of autophagic complexes or HMGB1 translocation may suppress autophagy and consequently overcome chemoresistance in leukaemia.
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Copy and paste a formatted citation
Spandidos Publications style
Kong Q, Xu L, Xu W, Fang J and Xu H: HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia. Int J Oncol 47: 161-170, 2015.
APA
Kong, Q., Xu, L., Xu, W., Fang, J., & Xu, H. (2015). HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia. International Journal of Oncology, 47, 161-170. https://doi.org/10.3892/ijo.2015.2985
MLA
Kong, Q., Xu, L., Xu, W., Fang, J., Xu, H."HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia". International Journal of Oncology 47.1 (2015): 161-170.
Chicago
Kong, Q., Xu, L., Xu, W., Fang, J., Xu, H."HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia". International Journal of Oncology 47, no. 1 (2015): 161-170. https://doi.org/10.3892/ijo.2015.2985
Copy and paste a formatted citation
x
Spandidos Publications style
Kong Q, Xu L, Xu W, Fang J and Xu H: HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia. Int J Oncol 47: 161-170, 2015.
APA
Kong, Q., Xu, L., Xu, W., Fang, J., & Xu, H. (2015). HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia. International Journal of Oncology, 47, 161-170. https://doi.org/10.3892/ijo.2015.2985
MLA
Kong, Q., Xu, L., Xu, W., Fang, J., Xu, H."HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia". International Journal of Oncology 47.1 (2015): 161-170.
Chicago
Kong, Q., Xu, L., Xu, W., Fang, J., Xu, H."HMGB1 translocation is involved in the transformation of autophagy complexes and promotes chemoresistance in leukaemia". International Journal of Oncology 47, no. 1 (2015): 161-170. https://doi.org/10.3892/ijo.2015.2985
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