Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21

  • Authors:
    • Xiaomei Li
    • Chenhui He
    • Changzhen Liu
    • Juan Ma
    • Pan Ma
    • Honglian Cui
    • Hua Tao
    • Bin Gao
  • View Affiliations

  • Published online on: May 14, 2015     https://doi.org/10.3892/ijo.2015.3005
  • Pages: 335-342
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Abstract

Adoptive transfer of NK cells has been widely applied clinically for cancer immunotherapy. However, the difficulties to obtain a large number of activated NK cells impede the successful application of such therapy. In the present study, we implemented a novel method involving the use of immobilized human 4-1BBL and interleukin-21 to amplify NK cells from the peripheral blood mononuclear cells (PBMCs) of healthy donors. Following stimulation for 21 days, we achieved considerable expansion of NK cells with high purity and strong cytotoxicity. This is the first time solid phase cytokines were used to augment NK cells, and this method has the advantage of no need to introduce feeder cells, without prior purification of NK cells and it effectively stimulated and expanded NK cells. The strategy of cell proliferation and activation could lead to a safer and more effective application of NK cells clinically.
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July-2015
Volume 47 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Li X, He C, Liu C, Ma J, Ma P, Cui H, Tao H and Gao B: Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21. Int J Oncol 47: 335-342, 2015
APA
Li, X., He, C., Liu, C., Ma, J., Ma, P., Cui, H. ... Gao, B. (2015). Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21. International Journal of Oncology, 47, 335-342. https://doi.org/10.3892/ijo.2015.3005
MLA
Li, X., He, C., Liu, C., Ma, J., Ma, P., Cui, H., Tao, H., Gao, B."Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21". International Journal of Oncology 47.1 (2015): 335-342.
Chicago
Li, X., He, C., Liu, C., Ma, J., Ma, P., Cui, H., Tao, H., Gao, B."Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21". International Journal of Oncology 47, no. 1 (2015): 335-342. https://doi.org/10.3892/ijo.2015.3005