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Article

miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells

  • Authors:
    • Kang He
    • Dongdong Tong
    • Si Zhang
    • Donghui Cai
    • Lumin Wang
    • Yang Yang
    • Ling Gao
    • Su'e Chang
    • Bo Guo
    • Tusheng Song
    • Ang Li
    • Chen Huang
  • View Affiliations / Copyright

    Affiliations: Department of Periodontology, Stomatology Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710004, P.R. China, Department of Genetics and Molecular Biology, Key Laboratory of Environment and Genes Related to Diseases, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Stomatology, First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710077, P.R. China, Department of Oral Maxillofacial Surgery, Stomatology Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 1528-1536
    |
    Published online on: August 27, 2015
       https://doi.org/10.3892/ijo.2015.3135
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Abstract

Dysregulation of microRNAs (miRNAs) has been associated with carcinogenesis in oral squamous cell carcinoma (OSCC). In the present study, we investigated the expression and function of miR-99b-3p in human OSCC. We found that the expression levels of miR-99b-3p decreased in 21 clinical OSCC samples (84%). Furthermore, ectopic expression of miR-99b-3p inhibited OSCC cell proliferation by downregulating glycogen synthase kinase-3β (GSK3β), an miR-99b-3p' target gene, at the mRNA and protein levels, both in vitro and in vivo. Moreover, the silencing of GSK3β recapitulated the cellular and molecular effects in a similar manner to the overexpression of miR-99b-3p, which included inhibition of OSCC cell proliferation and suppression of p65 (RelA) and G1 regulators (cyclin D1, CDK4 and CDK6) in vitro. Our data suggest that miR-99b-3p functions as a tumor suppressor in OSCC via GSK3β downregulation.
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Copy and paste a formatted citation
Spandidos Publications style
He K, Tong D, Zhang S, Cai D, Wang L, Yang Y, Gao L, Chang S, Guo B, Song T, Song T, et al: miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells. Int J Oncol 47: 1528-1536, 2015.
APA
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y. ... Huang, C. (2015). miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells. International Journal of Oncology, 47, 1528-1536. https://doi.org/10.3892/ijo.2015.3135
MLA
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y., Gao, L., Chang, S., Guo, B., Song, T., Li, A., Huang, C."miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells". International Journal of Oncology 47.4 (2015): 1528-1536.
Chicago
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y., Gao, L., Chang, S., Guo, B., Song, T., Li, A., Huang, C."miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells". International Journal of Oncology 47, no. 4 (2015): 1528-1536. https://doi.org/10.3892/ijo.2015.3135
Copy and paste a formatted citation
x
Spandidos Publications style
He K, Tong D, Zhang S, Cai D, Wang L, Yang Y, Gao L, Chang S, Guo B, Song T, Song T, et al: miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells. Int J Oncol 47: 1528-1536, 2015.
APA
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y. ... Huang, C. (2015). miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells. International Journal of Oncology, 47, 1528-1536. https://doi.org/10.3892/ijo.2015.3135
MLA
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y., Gao, L., Chang, S., Guo, B., Song, T., Li, A., Huang, C."miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells". International Journal of Oncology 47.4 (2015): 1528-1536.
Chicago
He, K., Tong, D., Zhang, S., Cai, D., Wang, L., Yang, Y., Gao, L., Chang, S., Guo, B., Song, T., Li, A., Huang, C."miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells". International Journal of Oncology 47, no. 4 (2015): 1528-1536. https://doi.org/10.3892/ijo.2015.3135
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