Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer

  • Authors:
    • Junyu He
    • Guangchao Zhu
    • Lu Gao
    • Pan Chen
    • Yuehua Long
    • Shan Liao
    • Hong Yi
    • Wei Yi
    • Zhen Pei
    • Minghua Wu
    • Xiaoling Li
    • Juanjuan Xiang
    • Shuping Peng
    • Jian Ma
    • Ming Zhou
    • Wei Xiong
    • Zhaoyang Zeng
    • Bo Xiang
    • Ke Tang
    • Li Cao
    • Guiyuan Li
    • Yanhong Zhou
  • View Affiliations

  • Published online on: September 2, 2015     https://doi.org/10.3892/ijo.2015.3146
  • Pages: 1725-1734
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Abstract

Gastric cancer is an aggressive disease that continues to have a daunting impact on global health. Fra-1 (FOSL1) plays important roles in oncogenesis in various malignancies. We investigated the expression of Fra-1 in gastric cancer (GC) tissues by qPCR, immunohistochemistry (IHC) and western blot technologies. The results showed that Fra-1 was overexpressed in gastric cancer tissues compared with the adjacent non‑cancerous tissues. To explore the possible mechanism of Fra-1 in GC, we elucidated the effect of Fra-1 in the apoptosis and cell cycle of gastric cancer cells, AGS, and found that a considerable decrease in apoptotic cells and increase of S phase rate were observed for AGS cells with Fra-1 overexpession. We identified and confirmed that Fra-1 affected the expression level of CTTN and EZR in vitro through LC-MS/MS analyses and western blot technology. Furthermore, we found that Fra-1 was correlated with dysregulation PI3K/Akt and p53 signaling pathway in gastric cancer tissues in vitro. Moreover, we found that Fra-1 overexpression affected the expression of PI3K, Akt, MDM2 and p53 in vivo. In summary, our results suggest that Fra-1 is upregulated in gastric cancer tissues and plays its function by affecting the PI3K/Akt and p53 signaling pathway in gastric cancer.
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November-2015
Volume 47 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
He J, Zhu G, Gao L, Chen P, Long Y, Liao S, Yi H, Yi W, Pei Z, Wu M, Wu M, et al: Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer. Int J Oncol 47: 1725-1734, 2015
APA
He, J., Zhu, G., Gao, L., Chen, P., Long, Y., Liao, S. ... Zhou, Y. (2015). Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer. International Journal of Oncology, 47, 1725-1734. https://doi.org/10.3892/ijo.2015.3146
MLA
He, J., Zhu, G., Gao, L., Chen, P., Long, Y., Liao, S., Yi, H., Yi, W., Pei, Z., Wu, M., Li, X., Xiang, J., Peng, S., Ma, J., Zhou, M., Xiong, W., Zeng, Z., Xiang, B., Tang, K., Cao, L., Li, G., Zhou, Y."Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer". International Journal of Oncology 47.5 (2015): 1725-1734.
Chicago
He, J., Zhu, G., Gao, L., Chen, P., Long, Y., Liao, S., Yi, H., Yi, W., Pei, Z., Wu, M., Li, X., Xiang, J., Peng, S., Ma, J., Zhou, M., Xiong, W., Zeng, Z., Xiang, B., Tang, K., Cao, L., Li, G., Zhou, Y."Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer". International Journal of Oncology 47, no. 5 (2015): 1725-1734. https://doi.org/10.3892/ijo.2015.3146