The antitumor mechanism of di-2-pyridylketone 2-pyridine carboxylic acid hydrazone and its copper complex in ROS generation and topoisomerase inhibition, and hydrazone involvement in oxygen-catalytic iron mobilization

  • Authors:
    • Tengfei Huang
    • Cuiping Li
    • Xingzhi Sun
    • Zhenfu Zhu
    • Yun Fu
    • Youxun Liu
    • Yanbin Yuan
    • Shaoshan Li
    • Changzheng Li
  • View Affiliations

  • Published online on: September 11, 2015     https://doi.org/10.3892/ijo.2015.3158
  • Pages: 1854-1862
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Abstract

Iron depletion and stimulation of iron-dependent free radical damage is a rapidly developing field for chelation therapy, but the iron mobilization from ferritin by chelators has received less attention. In this study, the di-2-pyridylketone 2-pyridine carboxylic acid hydrazone (DPPCAH) and its copper complex was prepared and characterized by NMR and MS spectra. The proliferation inhibition assay showed that both DPPCAH and its copper complex exhibited selectively proliferation inhibition for HepG2 (IC50, 4.6±0.2 µM for DPPACH and 1.3±0.2 µM for its copper complex), but less inhibition for HCT-116 cell line (IC50, >100 µM for DPPACH and 7.8±0.4 µM for its copper complex). The mechanistic studies revealed that DPPACH could remove iron from ferritin in a oxygen-catalytic manner, and contributed to redox activity of labile iron pool (LIP), that is less reported for the chelators that possess significant biological activity. The reactive oxygen species (ROS) generation and DNA cleavage assay in vitro and in vivo showed that both DPPACH-Fe(II) and DPPACH-Cu were redox-active species, indicating that ROS may mediate their antitumor activity. Further study revealed that both DPPACH and its copper complex displayed certain degree of inhibition of type II topoisomerase (Top) which contributed to their antitumor activity. Thus, the mechanism that iron mobilization by DPPACH from ferritin contributed to LIP was proposed, and both DPPACH and its copper complex were involved in ROS generation and Top II inhibition for their antitumor activities.

Introduction

It is well-known that iron is an essential element and plays a crucial role in cellular proliferation and DNA synthesis. Neoplastic cells have high requirement for iron for their significantly elevated expression of the transferrin receptor 1 (1) and the higher levels of the Fe-containing enzyme. The iron used for biological synthesis is from labile iron pool (LIP) that also involve in the production of reactive oxygen species (ROS) (2). Thus, iron depletion and stimulation of iron-dependent free radical damage is a rapidly developing field for anticancer drug discovery (3,4).

It has been demonstrated that the mobilization of iron from ferritin requires the use of a suitable reductant to chemically reduce the ferrihydrite (Fe2O3·nH2O) phosphate mineral core. Besides a chemical reducing agent, light or ionizing radiation is also used to trigger the mobilization of iron ions from ferritin (5). Small bidentate Fe(III) chelate ligands are capable of removing iron from the ferritin inorganic core via direct extraction followed by diffusion of the Fe(III)-chelate out of the protein shell (6), however, to achieve significant iron release, high ligand concentration (3.5–100 mM) is required due to the relatively low affinity of these ligands to iron. Recently, Bou-Abdallah et al reported that 2,6-bis[(hydroxymethyl) amino]-1,3,5-triazine (BHT) could rapidly release iron from human recombinant ferritin in the presence of oxygen, and proposed a mechanism that BHT-Fe(II) mediates the regeneration of superoxide radical, which used for reduction of ferritin (7). The finding hints that some chelators can make contribution to content of labile iron. In thermodynamics, the redox potential of iron is decreased once it is chelated, that the conversion between oxidation states of iron [Fe(III)/Fe(II)] is easily occurred. This is fundamental for catalyzing a plethora of biochemical redox processes. However, whether the chelators that possess antitumor activity are also involved in oxygen-catalytic iron mobilization is less clear.

Desferoxamine (DFO) is an iron chelator and used in clinic to treat iron overload disease and in combination therapy of doxorubicin to reduce the acute doxorubicin-induced cardiac, renal and hepatic toxicity. The antiproliferative effects of desferoxamine (DFO) are mediated by an intracellular pool of iron (8). Dp44mt is a chelator of thiosemicarbazone of di-2-pyridylketone, exhibiting excellent antitumor activities via multiple models of action, such ROS induction (9), topoisomerase inhibition (10), and lysosome inhibition (11). Similarly the pyridine carboxylic acid hydrazones of di-2-pyridylketone are also iron chelators, displaying excellent antitumor activity through ROS generation or other mechanism. The extensive mechanistic studies support that the ROS production is involved and is labile iron-dependent, yet the contribution of chelators to LIP is not fully understood. We hypothesize that the ROS induction by some tridentate chelators, such as Dp44mt, may be involved in iron mobilization and contribute to LIP (7). To test the hypothesis, a Dp44mt analog, di-2-pyridylketone 2-pyridine carboxylic acid hydrazone (DPPACH) was synthesized to probe this possibility. In the study, the iron mobilization mediated by DPPACH from ferritin at different conditions was investigated, the results indicated that the DPPACH at lower concentration can release iron from ferritin oxygen-dependently, which accordingly increased ROS production. The results from ROS assay in vitro and in vivo as well as DNA fragmentation indicate that the chelated Fe(II) and copper are redox active, revealing the active species may be related to their antitumor activities. Thus, DPPACH copper complex was also evaluated in the study. The additional DNA relaxation assay showed that both DPPACH and its copper complex possess weaker topoisomerase IIa (Top IIa) inhibition. The above suggested that DPPACH and its copper complex exhibit antitumor activities via ROS induction and Top inhibition.

Materials and methods

Materials

All reactants and solvents were AR grade. MTT, ethidium bromide (EB), di-2-pyridylketone, RPMI-1640, horse spleen ferritin and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). The superoxide dismutase (SOD) was obtained from the Beyotime Institute of Biotechnology (Beijing, China).

Preparation of DPPACH

DPPACH was made by refluxing di-2-pyridylketone with 2-pyridine carboxylic acid hydrazide in absolute ethanol for 4 h. The 2-pyridine carboxylic acid hydrazide was prepared as described (12). 1HNMR: 15.0 (s, NH), 8.96 (d, H, J=4 Hz), 8.753 (d, H, J=4 Hz), 8.62 (d, H, J=4 Hz), 8.62 (d, H, J=4 Hz), 8.18 (d, H, J=8 Hz), 8.10 (d, H, J=8 Hz), 8.03 (tri, H, J=12 Hz), 7.70 (tri, H, J=12 Hz), 7.64 (tri, H, J=12 Hz) and 7.50 (tri, H, J=12 Hz). ESI-MS (microTOF-Q III; Bruker Corp., Billerica, MA, USA): m/z: 304.1176 (M+H, calcd: 304.1198). For copper complex (m/z): 401.0104 (M+CuCl, calcd:401.0104, weak): 365.0338 (M-H+Cu, calcd: 365.0338, major peak). The structures of DPPACH and its copper in the study are shown below.

Determination of the mole ratio of DPPACH to the Cu(II) complex

The stoichiometry of the reaction between DPPACH and copper chloride was studied by Job's method of continuous variations (13). For this method, different volumes (0, 20, 40, 60, 80, 100, 120, 140, 160 and 180 μl) of 1 mM CuCl2 was added with different volumes (200, 180, 160, 140, 120, 100, 80, 60, 40 and 20 μl) of 1 mM DPPACH and diluted with water in 5 ml standard volumetric flask. The absorbance was recorded on a Shimadzu UV-2450 spectrophotometer. The absorbance at 366 nm against the mole fraction of the mole ratio of Cu(II) (or DPPACH) was plotted and regressed linearly (SigmaPlot 10.0).

Iron mobilization by DPPACH

The iron release experiments were conducted in 0.05 M Tris-HCl, 50 mM NaCl, pH 7.4, 10 μl ferritin (12.5 mg/ml) and varied concentration of DPPACH (or 3 unit SOD) in a total 1 ml volume. The kinetics of iron release from ferritin was monitored by the increase in the characteristic metal-to-ligand charge transfer (MLCT) absorption bands of the corresponding Fe(II)-chelate complexes (350 nm for DPPACH). The absorbance was measured every 5 min on a Shimadzu UV-2450 spectrophotometer with thermostatic circulating device at 37°C.

ROS detection in vitro and in vivo

H2DCF-DA was converted to dichlorofluorescin (DCF) according to literature (14). Briefly, 0.25 ml of 2 mM H2DCF-DA in absolute ethanol was added to 2.0 ml of 10 mM NaOH and allowed to stand at room temperature for 30 min. The hydrolysate was then neutralized with 10 ml of 25 mM sodium phosphate buffer (pH 7.2), kept on ice until used. Reaction mixtures contained either single reagent or multicomponent in 50 mM sodium phosphate buffer (pH 7.4) with total 4 ml volume, i.e., 0.4 μM DCF, or with 6.25 μM NH4FeSO4 (or CuCl2 or DPPACH) and 200 μM H2O2 (1 mM) for the Fenton reactions. The fluorescence was measured on a FC-960 spectrofluorimeter (excitation at 488 and emission at 525 nm) in a 10 min time course at room temperature.

The intracellular ROS production was measured as recommended (Beyotime Institute of Biotechnology). Approximately 106 HepG2 cells were collected and washed by PBS. The cell pellet was re-suspended in H2DCF-DA containing serum-free culture medium and incubated for 30 min. The stained cells were re-collected and washed with serum-free culture medium. Then 100 μl of the cell culture was transferred to individual PCR tube and the test compound (or positive control) was added, following 1-h incubation, the cell suspension was used directly for ROS determination on a FC-960 spectrofluorimeter by excitation at 488 and emission at 525 nm.

DNA cleavage assay

The DNA cleavage experiment was conducted by gel electrophoresis. The reaction mixture was prepared as follows: 1 μl of pUC18 DNA (0.5 μg/μl), 5 μl of 1 mM DPPACH-Fe(II) [Cu(II); 1:1 molar ratio] in 8% dimethyl sulphoxide (DMSO), and 1 μl of H2O2 (4 mM) followed by diluting with buffer (50 mM Tris-HCl, pH7.2 with 50 mM NaCl) to a total volume of 25 μl. The reaction mixture was incubated at 37°C for 1 h. The 10 μl of the reaction mixture with loading buffer were placed on 1.5% agarose gel and subjected to electrophoresis. The bands were visualized by EB stain and photographed on a Tocan 360 gel imager (version 3.2.1 software).

The comet assay was adapted from the literature as described (15). HepG2 cells were treated with or without the investigated compounds (12.5, 25 μM for DPPACH or 3, 6 μM for DPPACH-Cu complex) with a 48-h incubation in a humidified atmosphere of 5% CO2. The cells were harvested by centrifugation after trypsinization and then embedded in 0.5% low melting-point agarose at a final concentration of 104 cells/ml. Twenty microliters of this cellular suspension was then spread onto duplicate frosted slides that had previously been covered with 1% normal melting-point agarose as a basal layer. Slides were allowed to solidify for 10 min at 4°C before being placed in lysis buffer for 1 h (2.5 M NaCl, 0.1 M ethylenediaminetetraacetic acid (EDTA), 0.01 M Tris, 1% Triton X-100, 10% DMSO, pH 10.0). After lysis, the slides were transferred into alkaline buffer for 40 min (0.001 M EDTA, 0.3 M NaOH, pH >13.0) to allow the DNA to unwind before migration at 0.66 V/cm and 300 mA for 30 min. All these steps were performed in the dark. After neutralisation in 0.4 M Tris-HCl, pH 7.4, slides were stored at 4°C until analysis within 24 h. Before analysis, the slides were stained with EB (20 μg/ml) and covered with a cover-slip. The Images was captured using fluorescent microscopy.

Cytotoxicity assay (MTT assay)

The stock solution of DPPACH (10 mM) was prepared in 80% DMSO, it was diluted to the required concentration with culture when used. The copper complex was made by mixing DPPACH with high concentration copper chloride based on 1:1 molar ratio and diluted to required concentration with water. HCT-116 and HepG2 were cultured in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS) and antibiotics. The cells collected during exponential-phase (5×103/ml) were seeded equivalently into a 96-well plate and the various amount of DPPACH (or its copper complex) was added after the cells adhered. Following 48 h incubation at 37°C in a humidified atmosphere of 5% CO2, 10 μl MTT solution (5 mg/ml) was added to each well, followed by further incubation of 4 h. The cell culture was removed by aspiration and 100 μl DMSO was added in each well to dissolve the formazan crystals. The measurement of absorbance of the solution that was related to the number of live cells was performed on a microplate reader (MK3; Thermo Fisher Scientific) at 570 nm. Percent growth inhibition was defined as the percent absorbance inhibition within appropriate absorbance in each cell line. The assay was performed in triplicate.

DNA Top activity assay

The nuclear extract from HepG2 cells was prepared as previously described (14). Nuclear extract (0.4 μg) was added to the Top reaction mixture containing 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 150 mM NaCl, 0.1% bovine serum albumin (BSA), 5% glycerol and 0.4 μg pUC18 and 1 μl (or 2, or 3 μl) test agent (1 mM DPPACH or 1 mM DPPACH-Cu complex in 8% DMSO) at a final volume of 20 μl. Following incubation at 37°C for 30 min, the reaction was terminated by adding 5 μl of stopping buffer (10% SDS, 0.025% bromophenol blue and 10% glycerol). The reaction products were analyzed by electrophoresis on 1% agarose gel using a TBE buffer with 0.1% SDS (89 mM Tris-HCl, 89 mM boric acid and 62 mM EDTA) at 45 V for 3 h, stained by EB (0.5 μg/ml) and photographed using a short wavelength UV lamp on Tocan 360 gel scanner (Shanghai Tiancheng Technology Co., Ltd., Shanghai, China). The assay was conducted in duplicates.

Molecular docking studies

The structure of human type II Top (3QX3) was obtained from RCSB Protein Data Bank. The structure of DPPACH was generated by Chemdraw (Chemdraw Ultra 8.0; CambridgeSoft, Cambridge, MA, USA). Similarly the structure of DPPACH-Cu complex was proposed based on stoichiometry obtained, mass spectrum and coordination geometry of Cu(II) ion in literature (17). The energy minimization was conducted by Chem3D (Chemdraw Ultra 8.0) (18). The resulting model was displayed in PyMol (The PyMOL Molecular Graphics System, version 1.4.1; Schrödinger, LLC, New York, NY, USA).

Molecular docking studies were performed by AutoDock Vina and AutoDock Tools based on the recommended procedure (19). Grid box was set to the center of etoposide model, and the grid box size for Mannich base models was set to 22, 24 and 28 for x, y and z axes, respectively. The DPPACH or its copper complex was set as a flexible ligand by using the default parameters of the AutoDock Tools. The optimal conformation of the ligand was generated by AutoDock Vina.

Results

Syntheses and characterization of DPPACH and its copper complex

To test the hypothesis that some tridentate chelators are involved in iron mobilization from ferritin oxygen-dependently, a tridentate chelator of DPPACH was prepared and characterized by NMR and MS, the compound used in the study is shown in Fig. 1A. The new chelator is an analog of di-2-pyridylketone isonicotinoyl hydrazone and less studied, and its metal chelating ability to copper or iron was investigated because the metal complexes formed may be ‘active species’ for their biological activity. The stoichiometric ratio between DPPACH and copper chloride was determined by Job's method of continuous variations. A new peak around 366 nm was due to the chelation of the DPPACH with Cu(II). The plot of absorbance versus mole fraction of DPPACH confirmed a new species (complex) with ratio 1:1 between DPPACH and copper ion (Fig. 2). Further supportive evidence from ESI-MS, indicated the predominated monomeric peak [Cu(DPPACH-H)]+ with an m/z=365.0338 was found, indicating the DPPACH in the copper complex was in enoled manner. A six-fold less minor peak at m/z=401.0104 was also detected in this mixture, suggesting a 1:1 ligand-to-copper complex was the active species. The structure of copper complex was proposed (Fig. 1B). The stoichiometric ratio of DPPACH/Fe(II) was not determined by the spectrometric method due to the varied ratio, yet the new peak (350 nm) was identified to be DPPACH-Fe(II) complex based on the UV-visible spectrum (Fig. 3A).

DPPACH involved in oxygen-catalytic iron mobilization from ferritin

To test whether DPPACH was capable of removing iron from the ferritin, the low concentration of DPPACH was used in the assay, as the direct extraction by chelators required much higher concentration (3.5–100 mM) to diffuse the Fe(III)-chelate out of the protein shell. The iron release from ferritin were traced by monitoring the increase of the characteristic MLCT absorption band which corresponded to Fe(II)-DPPACH complexes (Fig. 3A, absorption at ~350 nm). As shown in Fig. 3B, the iron release was increased with increasing DPPACH in the time course, and the profiles of iron release were in an exponential rising manner. To determine whether superoxide radical was involved, the SOD was added, showing that SOD inhibited significantly the iron release thus indicating the involvement of ROS in the process of iron removal from ferritin. To corroborate this finding, iron release experiments under anaerobic conditions was conducted, the data indicated almost complete inhibition of iron release by DPPACH, suggesting that molecular oxygen is required to initiate the generation of ROS which then reduce the mineral core and release Fe(II), Those supporting that DPPACH could release iron from ferritin in a oxygen-catalytic manner as previously reported (7).

DPPACH and its copper complex induce ROS generation

Since DPPACH was able to remove iron from ferritin, the formed ferrous iron complex may act as either contributor to LIP or bystander, depending on the redox characteristic of iron DPPACH complex, thus DPPACH induction of ROS was an indicative factor. DCF was chosen to assess ROS generation. As shown in Fig. 4A, fluorescence intensity of DCF was the highest in the presence of DPPACH, indicating that iron DPPACH complex more efficiently induced ROS formation in Fenton reaction and the complex was redox-active. In contrast to the DPPACH-Fe(II), DPPACH-Cu(II) was also active in ROS generation, but weaker in terms of fluorecence intensity, however, once DPPACH-Cu(II) was partly reduced by ascorbic acid (Vc), the DPPACH-Cu(I) was efficient in ROS generation compared to its oxidized form. Based on the above results, we speculated that the DPPACH-Fe(II) complex may contribute to LIP when ferritin and DPPACH co-existed in cells. Fig. 5F shows the ROS induction by the investigated agents in cellular level (in vivo), both DPPACH and its copper complex induced ROS generation in a concentration dependent manner, indicating that the excess ROS induction occurred in vivo. It was noted that the fluorescent intensities of DCF from copper complex treated cells were significantly greater than that of DPPACH, which might be related to the redox feature of Cu2+/1+ complexes, because the Cu(I) complex is able to also react with molecular oxygen to form the superoxide radical except with H2O2 (20).

DNA fragmentation induced by DPPACH and its copper complex

It has been demonstrated that ROS caused oxidative damage of DNA, protein and lipid. The oxidative breakage of pUC18 by the investigated agents was conducted by agarose gel electrophoresis. As expected in the assay, the supercoiled DNA decreased, and cleaved DNA increased, with increasing concentration, supporting that ROS is involved in DNA fragmentation (Fig. 4B). Similarly the effect of the agents on DNA integrity of HepG2 cells also supported the results from that in vitro. As shown in Fig. 5A–E, a typical image of a migrated cell nucleus with DNA strand breaks had migrated sufficiently to form the ‘teardrop’ tail, and the greater portion of the DNA had fragmented in concentration-dependent manner. It was noted that the copper complex displayed stronger effect on nucleic DNA damage compared to DPPACH (Fig. 5D and E), showing that the copper complex more efficiently induced DNA fragmentation, that was consistent with the results from ROS generation in vivo (Fig. 5F), yet the underlying mechanism is not fully understood.

The cytotoxicity of the DPPACH and its copper complex

The DPPACH and its copper complex induced ROS generation and oxidative damage of DNA in vitro and in vivo, this motivated us to probe their potent antitumor activity. The inhibitory effects of the agents on the proliferation of the HepG2 and HCT-116 cell lines was evaluated and the dose-response curves are depicted in Fig. 6. As shown in Fig. 6, DPPACH had significant growth inhibition for HepG2 (IC50: 4.6±0.2 μM), but the maximal inhibition was ~60%. It was noted that the DPPACH had poor growth inhibition for HCT-116 cell lines (IC50: >100 μM), the maximal inhibition was only ~40%, showing a certain degree of selectivity between the two cell lines for DPPACH. The DPPACH copper complex exhibited excellent antitumor activity for each cell line. The IC50 of the copper complex was 1.3±0.5 μM for HepG2 cell, an ~3-fold increase in proliferation inhibition compared to that of DPPACH. Similar trend against HCT-116 cell line was also observed, the IC50 of the copper complex was 7.8±0.4 μM, an ~15-fold increase. The data indicated that the antitumor activities exhibited by the agents at least was partly correlated to their ability of ROS induction in vivo.

DNA relaxation inhibition

The excellent antitumor activities for some copper complexes have been recognized, yet the underlying mechanism was not defined. In the present study, the antitumor activities of copper complex was not fully correlated to its ability of ROS generation, implying that some other mechanism occurred. Dp44mt was reported as Top inhibitor, as its analog, the DPPACH might have similar action. To determine whether the DPPACH and its copper complex recapitulate such activity, pUC18 plasmid DNA was incubated with nucleic extract in the presence of a varied concentration of the investigated agents, and reaction products were analyzed by gel electrophoresis. As shown in Fig. 7A, the DPPACH and its copper complex did not exhibit any inhibition for Top I at given condition (without ATP in the assay), while in the presence of ATP, both displayed certain degree of inhibition based on EtB pre-stained agarose gel after 4 h electrophoresis (Fig. 7B). As shown in Fig. 7B, the relaxed DNA was observed and migrated faster than the supercoiled DNA in the control line, the cleaved DNA was increased with increasing concentration of both reagents, and the copper complex was stronger than DPPACH. The Top II cleavage complex was identified by comparison with the positive control of etoposide, however in our experiment the Top II cleavage complex was not observed. This situation has occurred in other reported cases due to lower Top II concentration used in the assay, but the cleaved DNA (Fig. 7B) increased with increasing of the agents, implying that there was the possibility of catalytic or ‘poisoner’ inhibition.

Molecular docking studies

Top inhibition of the DPPACH and its copper complex in vitro prompted us to probe the potent structural basis, and molecular docking was conducted. The human type II Top crystal structure (PDB ID: 3QX3) was from RCSB Protein Data Bank. In order to evaluate the accuracy of our docking protocol, etoposide was re-docked into the Top-DNA complexes based on a recommended procedure (Fig. 8A). The Top-etoposide complex derived from docking simulation showed that the docked etoposide was almost fully superimposed on the native co-crystallized one (Fig. 8A). Following the same protocol DPPACH and its copper complex were individually docked into Top II (Fig. 8B and D), the simulating affinity energies for them were −10.6 and −11.2 kcal/mol, respectively. Compared to that of docked etoposide, −14.8 kcal/mol, the affinity energies of the DPPACH and its copper complex with the Top were weaker than that of the clinically used drug. The superimposition of DPPACH-Cu complex and DPPACH on the co-crystallized etoposide are presented in Fig. 8C and E. The affinity energies from docking simulation were correlated to their Top inhibition activity.

Discussion

Cytosolic LIP is a pool of chelatable and redox-active iron, which is transitory and serves as a crossroad of cell iron metabolism (21). Release of intracellular iron from iron-sequestering proteins facilitates to the increases in redox-active LIP (22). Direct extraction from ferritin by some bidentate chelators has been well documented, and in the process the oxidative state of iron is not changed (6). Recent finding that BHT chelator is involved in oxygen catalyzed iron mobilization from ferritin may changevthe above concept (7), prompting that some chelators are capable of removing iron from ferritin of the cell, however, this contribution from chelators that possess antitumor activity has been neglected. The aroylhydrazones and thiosemicarbazones have been demonstrated selective antiproliferative activity against tumor cells (23,24), which have been attributed to their induction of cellular oxidative stress by Fenton-type free radical generation (23). Whether the chelators are involved in iron mobilization was not studied. We speculate some tridentate chelators may act as iron releaser from ferritin in an oxygen-catalytic manner, contributing to LIP and corresponding biological activity. To address this issue, the DPPACH, an analog of di-2-pyridylketone isonicotinoyl hydrazone was synthesized and evaluated on iron mobilization. The results demonstrated that DPPACH released iron from ferritin at lower concentration and was more efficient than the reported compounds. The addition of SOD inhibited significantly the iron release, indicating the involvement of ROS during iron release from ferritin. ROS could be a superoxide radical from aerobic environment, if so, the iron mobilization would be blocked under anaerobic conditions. Our data support a mechanism that DPPACH mobilizes iron from ferritin in an oxygen-dependent manner, which may also contribute to its ROS induction.

It has been demonstrated that the antiproliferative activity of some chelators relates to their ability to enhance redox activity of cellular iron (23). However, it is also found that there are redox-inactive species after chelator-iron complexes formed due to the redox potential outside the accessible range for redox cycling (25). Thus, determination of the redox activity of iron in the presence of the DPPACH in vitro is required. The fluorescent intensity of DCF is widely used for ROS detection is indicative of redox activity of the agent (26). Our data (Fig. 3A) clearly showed that the DPPACH-Fe(II) complex was more efficient to boost the ROS generation among the test compounds, indicating that it was a redox active species. The DPPACH-Cu(II) complex induced ROS production in the absence or presence of Vc was also evaluated. In contrast to the iron complex, the copper complex exhibited weak ability in ROS generation, however, the addition of Vc significantly increased its capability, indicating that the DPPACH-Cu complexes at different oxidation state are redox-active. It was noted that DPPACH-Cu complex at lower concentration induced efficiently ROS generation in vivo (Fig. 5F), which were found in many studies (27,28). Based on above results, we propose the pathways of ROS generation for DPPACH and its copper complex (Fig. 9), in which an oxygen-catalytic iron mobilization from ferritin was involved for DPPACH, and the formed ferrous complex may also participate in Fenton type ROS generation (Fig. 9). The mechanism related to copper complex was included in Fig. 9 to increase our understanding of the relationship between ROS and antiproliferative activity of chelators. ROS induce chromatin dysfunction such as single- and double-strand DNA fragmentation, leading to cell death through apoptosis or necrosis as have been documented (29,30). DNA cleavage caused by the DPPACH-Fe(II) and DPPACH-Cu were fully correlated to that from ROS assay in vitro. The difference in DNA fragmentation in the comet assay, was noted as the DNA cleavage required lower concentration of the copper comple. Even though slight difference in ROS generation in vitro and in vivo was observed, cellular DNA cleavage is consistent with their ability of ROS induction in vivo (Fig. 5F). The data supported that DPPACH and its copper exhibited antitumor activity via ROS generation.

Top inhibitors are important targets for drug design to disturb growth of tumor cells. Clinically used Top inhibitors are mostly aromatic fused organic compounds (31), including some metal complexes (32). The iron chelator Dp44mt was proposed to exhibit its antitumor activity via Top inhibition although it was reported to possess paradoxical function (33). In the present study, both DPPACH and its copper complex exhibited certain degree of Top IIα inhibition. In a previous study, we found that the copper complex of 2-pyridinecarboxaldehyde 2-pyridinecarboxylic acid hydrazone, an analog of DPPACH, possessed dual inhibition of Top (34), the slight difference of ligand in structure revealed their structure active relationship. Our data demonstrated that the antitumor mechanism for DPPACH and its copper complex were involved in type II Top inhibition and ROS generation, both contributed to DNA cleavage, thus their antitumor activities at least partly contributed to Top inhibition. Due to lack of evidence from crystal structure, how the compounds interact with Top, via competitive ATP binding or the DNA complex or allosteric effect to disturb unwinding DNA helix are unknown. For insight into the molecular mechanism, an in silico study to simulate the potent interaction between the compound and the DNA-Top complex may be helpful. AutoDock software is widely used to predict the binding information of test compounds between enzyme and the inhibitor (35). Based on the recommended procedure, the DPPACH and its copper were docked into the structure of human Top IIa. A square planar geometry of copper complex was chosen based on a similar crystal structure (17). It is interesting that the affinity energy of DPPACH was smaller than that of its copper complex, which was consistent with the results from top inhibition.

In conclusion, DPPACH has the ability to remove iron from ferritin in a oxygen-catalytic manner and forms a redox-active iron complex. Moreover, the chelated iron may also be from LIP, both contribute to redox activity of LIP, ROS generation and antitumor activity, but the contribution of the chelator (DPPACH) in iron mobilization from ferritin should not be neglected. The cellular DNA fragmentation is related to both Top II inhibition and ROS induction of DPPACH and its copper complex. However, the contributing fraction of DPPACH-Fe(II) from ferritin to its antitumor activity and copper complex induced ROS was not defined, and require more study in the future.

Acknowledgements

The authors would like to thank Miss Haiyan Yu, Yanqiu Ma, Shuang Wang and Xia Wu, undergraduates from the Department of Pharmacy of San Quan College, Xinxiang Medical University for their help in ROS assay. The present study was supported by grants from the Henan Science and Technology Agency (no. 210073), the Xinxiang Medical University (no. 505026) and the Plan of Health Scientific and Technological Innovation Talents (no. 2109901) of Henan Province for Shaoshan Li.

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November-2015
Volume 47 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Huang T, Li C, Sun X, Zhu Z, Fu Y, Liu Y, Yuan Y, Li S and Li C: The antitumor mechanism of di-2-pyridylketone 2-pyridine carboxylic acid hydrazone and its copper complex in ROS generation and topoisomerase inhibition, and hydrazone involvement in oxygen-catalytic iron mobilization. Int J Oncol 47: 1854-1862, 2015.
APA
Huang, T., Li, C., Sun, X., Zhu, Z., Fu, Y., Liu, Y. ... Li, C. (2015). The antitumor mechanism of di-2-pyridylketone 2-pyridine carboxylic acid hydrazone and its copper complex in ROS generation and topoisomerase inhibition, and hydrazone involvement in oxygen-catalytic iron mobilization. International Journal of Oncology, 47, 1854-1862. https://doi.org/10.3892/ijo.2015.3158
MLA
Huang, T., Li, C., Sun, X., Zhu, Z., Fu, Y., Liu, Y., Yuan, Y., Li, S., Li, C."The antitumor mechanism of di-2-pyridylketone 2-pyridine carboxylic acid hydrazone and its copper complex in ROS generation and topoisomerase inhibition, and hydrazone involvement in oxygen-catalytic iron mobilization". International Journal of Oncology 47.5 (2015): 1854-1862.
Chicago
Huang, T., Li, C., Sun, X., Zhu, Z., Fu, Y., Liu, Y., Yuan, Y., Li, S., Li, C."The antitumor mechanism of di-2-pyridylketone 2-pyridine carboxylic acid hydrazone and its copper complex in ROS generation and topoisomerase inhibition, and hydrazone involvement in oxygen-catalytic iron mobilization". International Journal of Oncology 47, no. 5 (2015): 1854-1862. https://doi.org/10.3892/ijo.2015.3158