TRIM21, a negative modulator of LFG in breast carcinoma MDA-MB-231 cells in vitro

Müller,Judith; Maurer,Viktor; Reimers,Kerstin; Vogt,Peter  M.; Bucan,Vesna

doi:10.3892/ijo.2015.3169

November-2015 Volume 47 Issue 5

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November-2015 Volume 47 Issue 5

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TRIM21, a negative modulator of LFG in breast carcinoma MDA-MB-231 cells in vitro

Authors:
- Judith Müller
- Viktor Maurer
- Kerstin Reimers
- Peter M. Vogt
- Vesna Bucan
View Affiliations / Copyright

Affiliations: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, D-30659 Hannover, Germany

Copyright: © Müller et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1634-1646
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Published online on: September 16, 2015

https://doi.org/10.3892/ijo.2015.3169
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Abstract

Lifeguard (LFG) is a transmembrane protein which is highly expressed in tissues of the hippocampus and the cerebellum, especially during postnatal development. This protein is responsible for the protection of neurons against Fas-induced apoptosis, and the same effect can be seen in tumor cells derived from mastocarcinoma. However, the molecular function of LFG and its regulation in the carcinogenesis of human breast cells remains to be elucidated. In the present study, we investigated the connection of the interaction of LFG within an array analysis of over 9,000 different proteins. Results showed an interaction between the proteins tripartite motif-containing 21 (TRIM21) and LFG and a negative regulatory effect of TRIM21 towards LFG on the protein level. Furthermore, Fas-induced apoptosis decreased upon the addition of TRIM21 to the cultured cells. These results revealed TRIM21 to be a negative modulator of LFG in cells of mastocarcinoma in vitro. For all analyses, MDA-MB-231 cells were used. The interaction of TRIM21 and LFG was analyzed by co-immunoprecipitation. To examine changes in regulatory processes, western blot analyses, real-time PCR, activity of apoptotic process and flow cytometric analyses were carried out.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

TRIM21, a negative modulator of LFG in breast carcinoma MDA-MB-231 cells in vitro

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Abstract

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