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International Journal of Oncology
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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Article

Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression

  • Authors:
    • Yan Duan
    • Winnie Wong
    • Sonja Courtney Chua
    • Hwee Lin Wee
    • Seng Gee Lim
    • Boon Tin Chua
    • Han Kiat Ho
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Faculty of Science, National University of Singapore, Singapore 117543, Republic of Singapore, Department of Gastroenterology and Hepatology, National University Health System, Singapore 119228, Republic of Singapore, Institute of Molecular and Cell Biology, Agency for Science Technology and Research, Singapore 138673, Republic of Singapore
  • Pages: 358-366
    |
    Published online on: November 13, 2015
       https://doi.org/10.3892/ijo.2015.3244
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Abstract

While various tyrosine kinases have been associated with the pathogenesis of hepatocellular carcinoma (HCC), the identification of a dominant therapeutic target among them remains a challenge. Here, we investigated the role of Tyro3, a relatively uncharacterized member of the TAM (Tyro3, Axl and Mer) receptor family. The present study aimed to profile and identify potential association between Tyro3 expression in HCC and cancer phenotypes. RNAs obtained from 55 HCC patients were quantified for Tyro3 expression in both cancerous tissue and the adjacent normal tissue. Expression profile was correlated with clinical data. These observations were further substantiated with in vitro HCC cell culture investigations.Tyro3 was strongly upregulated (>2-fold elevation) in the tumor tissue of ~42% of the patients. It was shown that higher expression level of Tyro3 was associated with the key tumor marker AFP, and the tumor diameter and liver injury marker ALT. Subsequent cell culture models indicated high expression in various HCC cell lines, in particular Hep3B. Gene silencing of Tyro3 in Hep3B effectively reduced cell proliferation, ERK phosphorylation and cyclin D1 expression, indicating a key in maintaining the proliferative state of these cells. Notably, silencing also suppressed the transcriptional and translational expression of HCC tumor marker AFP. Overall, these data suggest that Tyro3 contributes significantly to tumor growth, aggressiveness and liver dysfunction. Inhibition of Tyro3 and its aberrant signaling in tumors with high expression could present new opportunities for HCC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Duan Y, Wong W, Chua SC, Wee HL, Lim SG, Chua BT and Ho HK: Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression. Int J Oncol 48: 358-366, 2016.
APA
Duan, Y., Wong, W., Chua, S.C., Wee, H.L., Lim, S.G., Chua, B.T., & Ho, H.K. (2016). Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression. International Journal of Oncology, 48, 358-366. https://doi.org/10.3892/ijo.2015.3244
MLA
Duan, Y., Wong, W., Chua, S. C., Wee, H. L., Lim, S. G., Chua, B. T., Ho, H. K."Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression". International Journal of Oncology 48.1 (2016): 358-366.
Chicago
Duan, Y., Wong, W., Chua, S. C., Wee, H. L., Lim, S. G., Chua, B. T., Ho, H. K."Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression". International Journal of Oncology 48, no. 1 (2016): 358-366. https://doi.org/10.3892/ijo.2015.3244
Copy and paste a formatted citation
x
Spandidos Publications style
Duan Y, Wong W, Chua SC, Wee HL, Lim SG, Chua BT and Ho HK: Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression. Int J Oncol 48: 358-366, 2016.
APA
Duan, Y., Wong, W., Chua, S.C., Wee, H.L., Lim, S.G., Chua, B.T., & Ho, H.K. (2016). Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression. International Journal of Oncology, 48, 358-366. https://doi.org/10.3892/ijo.2015.3244
MLA
Duan, Y., Wong, W., Chua, S. C., Wee, H. L., Lim, S. G., Chua, B. T., Ho, H. K."Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression". International Journal of Oncology 48.1 (2016): 358-366.
Chicago
Duan, Y., Wong, W., Chua, S. C., Wee, H. L., Lim, S. G., Chua, B. T., Ho, H. K."Overexpression of Tyro3 and its implications on hepatocellular carcinoma progression". International Journal of Oncology 48, no. 1 (2016): 358-366. https://doi.org/10.3892/ijo.2015.3244
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