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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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April-2016 Volume 48 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes

  • Authors:
    • Mengjing Zhao
    • Cai Wei
    • Xue Yang
    • Juan Zhou
    • Jing Wang
    • Fang Gu
    • Ting Lei
    • Yide Qin
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China, Department of Pharmacy, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
  • Pages: 1721-1729
    |
    Published online on: February 12, 2016
       https://doi.org/10.3892/ijo.2016.3390
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Abstract

Some bioactive peptides derived from natural resources or synthesized by rational design have been proved to have very good anticancer effect. We studied the inhibition of PGPIPN, a hexapeptide derived from bovine β-casein, on the invasion and metastasis of human ovarian cancer cells in vitro and its molecular mechanism. The human ovarian cancer cells studied include the cell line SKOV3 as well as the primary ovarian cancer cells from ovarian tumor tissues of 37 patients at initial debulking surgery, diagnosed as serous ovarian adenocarcinoma. We showed that PGPIPN inhibited the invasion of ovarian cancer cells with Transwell chamber assay, the migration of ovarian cancer cells with cell scratch assay and colony formation of ovarian cancer cells. The expression (mRNAs and proteins) of genes relevant to invasion and metastasis, MTA1, and NM23H1 were analyzed by real-time PCR and western blotting. PGPIPN repressed the expression of MTA1, and promoted NM23H1. The effects of PGPIPN were dose-dependent. Thus, our study suggests that PGPIPN is a potential therapeutic agent for adjuvant therapy of human malignant ovarian tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao M, Wei C, Yang X, Zhou J, Wang J, Gu F, Lei T and Qin Y: The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes. Int J Oncol 48: 1721-1729, 2016.
APA
Zhao, M., Wei, C., Yang, X., Zhou, J., Wang, J., Gu, F. ... Qin, Y. (2016). The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes. International Journal of Oncology, 48, 1721-1729. https://doi.org/10.3892/ijo.2016.3390
MLA
Zhao, M., Wei, C., Yang, X., Zhou, J., Wang, J., Gu, F., Lei, T., Qin, Y."The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes". International Journal of Oncology 48.4 (2016): 1721-1729.
Chicago
Zhao, M., Wei, C., Yang, X., Zhou, J., Wang, J., Gu, F., Lei, T., Qin, Y."The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes". International Journal of Oncology 48, no. 4 (2016): 1721-1729. https://doi.org/10.3892/ijo.2016.3390
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao M, Wei C, Yang X, Zhou J, Wang J, Gu F, Lei T and Qin Y: The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes. Int J Oncol 48: 1721-1729, 2016.
APA
Zhao, M., Wei, C., Yang, X., Zhou, J., Wang, J., Gu, F. ... Qin, Y. (2016). The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes. International Journal of Oncology, 48, 1721-1729. https://doi.org/10.3892/ijo.2016.3390
MLA
Zhao, M., Wei, C., Yang, X., Zhou, J., Wang, J., Gu, F., Lei, T., Qin, Y."The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes". International Journal of Oncology 48.4 (2016): 1721-1729.
Chicago
Zhao, M., Wei, C., Yang, X., Zhou, J., Wang, J., Gu, F., Lei, T., Qin, Y."The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes". International Journal of Oncology 48, no. 4 (2016): 1721-1729. https://doi.org/10.3892/ijo.2016.3390
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