Inhibitory role of TRIP-Br1 oncoprotein in hypoxia-induced apoptosis in breast cancer cell lines

  • Authors:
    • Chengping Li
    • Samil Jung
    • Young Yang
    • Keun-Il Kim
    • Jong-Seok Lim
    • Chung-Il Cheon
    • Myeong-Sok Lee
  • View Affiliations

  • Published online on: March 24, 2016     https://doi.org/10.3892/ijo.2016.3454
  • Pages: 2639-2646
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Abstract

TRIP-Br1 oncoprotein is known to be involved in many vital cellular functions. In this study, we examined the role of TRIP-Br1 in hypoxia-induced cell death. Exposure to the overcrowded and CoCl2-induced hypoxic conditions increased TRIP-Br1 expression at the protein level in six breast cancer cell lines (MCF7, MDA-MB-231, T47D, Hs578D, BT549, and MDA-MB-435) but resulted in no significant change in three normal cell lines (MCF10A, MEF and NIH3T3). Our result revealed that CoCl2-induced hypoxia stimulated apoptosis and autophagy, in which TRIP-Br1 expression was found to be upregulated. Interestingly, TRIP-Br1 silencing in the MCF7 and MDA-MB-231 cancer cells accelerated apoptosis and destabilization of XIAP under the CoCl2-induced hypoxic condition, implying that TRIP-Br1 may render cancer cells resistant to apoptosis through the stabilization of XIAP. We also propose that TRIP-Br1 seems to be upregulated at least partly as a result of the inhibition of PI3K/AKT signaling pathway and the overexpression of HIF-1α. In conclusion, our findings suggest that TRIP-Br1 functions as an oncogenic protein by providing cancer cells resistance to the hypoxia-induced cell death.
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June-2016
Volume 48 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Li C, Jung S, Yang Y, Kim K, Lim J, Cheon C and Lee M: Inhibitory role of TRIP-Br1 oncoprotein in hypoxia-induced apoptosis in breast cancer cell lines. Int J Oncol 48: 2639-2646, 2016
APA
Li, C., Jung, S., Yang, Y., Kim, K., Lim, J., Cheon, C., & Lee, M. (2016). Inhibitory role of TRIP-Br1 oncoprotein in hypoxia-induced apoptosis in breast cancer cell lines. International Journal of Oncology, 48, 2639-2646. https://doi.org/10.3892/ijo.2016.3454
MLA
Li, C., Jung, S., Yang, Y., Kim, K., Lim, J., Cheon, C., Lee, M."Inhibitory role of TRIP-Br1 oncoprotein in hypoxia-induced apoptosis in breast cancer cell lines". International Journal of Oncology 48.6 (2016): 2639-2646.
Chicago
Li, C., Jung, S., Yang, Y., Kim, K., Lim, J., Cheon, C., Lee, M."Inhibitory role of TRIP-Br1 oncoprotein in hypoxia-induced apoptosis in breast cancer cell lines". International Journal of Oncology 48, no. 6 (2016): 2639-2646. https://doi.org/10.3892/ijo.2016.3454