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Article

GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax

  • Authors:
    • Kateřina Vlčková
    • Jiri Réda
    • Lubica Ondrušová
    • Mohammad Krayem
    • Ghanem Ghanem
    • Jiri Vachtenheim
  • View Affiliations / Copyright

    Affiliations: Laboratory of Transcription and Cell Signaling, Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic, LOCE-Institut Jules Bordet, Université Libre de Bruxelles, B-1000 Brussels, Belgium
  • Pages: 953-960
    |
    Published online on: July 1, 2016
       https://doi.org/10.3892/ijo.2016.3596
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Abstract

MEK kinase inhibitors (trametinib and selumetinib) or kinase inhibitors directed against mutated BRAF(V600E) (vemurafenib and dabrafenib) have initial encouraging effects in the treatment of melanoma but acquired resistance appears almost invariably after some months. Studies revealed mutually exclusive NRAS and BRAF activating mutations driving the MAPK/ERK pathway among human melanomas. Although combination therapy exerts significantly better antitumor cell efficacy, complete remission is rarely achieved. To employ an alternative approach, we have targeted the Hedgehog/GLI pathway, which is deregulated in melanomas, through the GLI1/2 inhibitor GANT61, alone or accompanied with the treatment by the BCL2 family inhibitor obatoclax in 9 melanoma cell lines. Thus, we targeted melanoma cells irrespective of their NRAS or BRAF mutational status. After GANT61 treatment, the cell viability was drastically diminished via apoptosis, as substantial nuclear DNA fragmentation was detected. In all tested melanoma cell lines, the combined treatment was more efficient than the application of each drug alone at the end of the cell growth with inhibitors. GANT61 was efficient also alone in most cell lines without the addition of obatoclax, which had only a limited effect when used as a single drug. In most cell lines, tumor cells were eradicated after 5-9 days of combined treatment in colony outgrowth assay. To conclude, GANT61 treatment might become a hopeful and effective anti-melanoma targeted therapy, especially when combined with the BCL2 family inhibitor obatoclax.
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1 

Marini KD, Payne BJ, Watkins DN and Martelotto LG: Mechanisms of Hedgehog signalling in cancer. Growth Factors. 29:221–234. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Robbins DJ, Fei DL and Riobo NA: The Hedgehog signal transduction network. Sci Signal. 5:re62012. View Article : Google Scholar : PubMed/NCBI

3 

Varjosalo M and Taipale J: Hedgehog: Functions and mechanisms. Genes Dev. 22:2454–2472. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Katoh Y and Katoh M: Hedgehog target genes: Mechanisms of carcinogenesis induced by aberrant hedgehog signaling activation. Curr Mol Med. 9:873–886. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Gonnissen A, Isebaert S and Haustermans K: Targeting the Hedgehog signaling pathway in cancer: Beyond Smoothened. Oncotarget. 6:13899–13913. 2015. View Article : Google Scholar : PubMed/NCBI

6 

McMillan R and Matsui W: Molecular pathways: The hedgehog signaling pathway in cancer. Clin Cancer Res. 18:4883–4888. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Li H, Li J and Feng L: Hedgehog signaling pathway as a therapeutic target for ovarian cancer. Cancer Epidemiol. 40:152–157. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Atwood SX, Chang AL and Oro AE: Hedgehog pathway inhibition and the race against tumor evolution. J Cell Biol. 199:193–197. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Stecca B, Mas C, Clement V, Zbinden M, Correa R, Piguet V, Beermann F, Ruiz I and Altaba A: Melanomas require HEDGEHOG-GLI signaling regulated by interactions between GLI1 and the RAS-MEK/AKT pathways. Proc Natl Acad Sci USA. 104:5895–5900. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Lauth M and Toftgård R: Non-canonical activation of GLI transcription factors: Implications for targeted anti-cancer therapy. Cell Cycle. 6:2458–2463. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Jenkins D: Hedgehog signalling: Emerging evidence for non-canonical pathways. Cell Signal. 21:1023–1034. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Shevde LA and Samant RS: Nonclassical hedgehog-GLI signaling and its clinical implications. Int J Cancer. 135:1–6. 2014. View Article : Google Scholar

13 

Agyeman A, Jha BK, Mazumdar T and Houghton JA: Mode and specificity of binding of the small molecule GANT61 to GLI determines inhibition of GLI-DNA binding. Oncotarget. 5:4492–4503. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Alexaki VI, Javelaud D, Van Kempen LC, Mohammad KS, Dennler S, Luciani F, Hoek KS, Juàrez P, Goydos JS, Fournier PJ, et al: GLI2-mediated melanoma invasion and metastasis. J Natl Cancer Inst. 102:1148–1159. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Steingrímsson E, Copeland NG and Jenkins NA: Melanocytes and the microphthalmia transcription factor network. Annu Rev Genet. 38:365–411. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, et al: Novel MITF targets identified using a two-step DNA microarray strategy. Pigment Cell Melanoma Res. 21:665–676. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Vachtenheim J and Borovanský J: ‘Transcription physiology’ of pigment formation in melanocytes: Central role of MITF. Exp Dermatol. 19:617–627. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Javelaud D, Alexaki VI, Pierrat MJ, Hoek KS, Dennler S, Van Kempen L, Bertolotto C, Ballotti R, Saule S, Delmas V, et al: GLI2 and M-MITF transcription factors control exclusive gene expression programs and inversely regulate invasion in human melanoma cells. Pigment Cell Melanoma Res. 24:932–943. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Pierrat MJ, Marsaud V, Mauviel A and Javelaud D: Expression of microphthalmia-associated transcription factor (MITF), which is critical for melanoma progression, is inhibited by both transcription factor GLI2 and transforming growth factor-β. J Biol Chem. 287:17996–18004. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Dennler S, André J, Alexaki I, Li A, Magnaldo T, ten Dijke P, Wang XJ, Verrecchia F and Mauviel A: Induction of sonic hedgehog mediators by transforming growth factor-beta: Smad3-dependent activation of Gli2 and Gli1 expression in vitro and in vivo. Cancer Res. 67:6981–6986. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Javelaud D, Alexaki VI, Dennler S, Mohammad KS, Guise TA and Mauviel A: TGF-β/SMAD/GLI2 signaling axis in cancer progression and metastasis. Cancer Res. 71:5606–5610. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Santini R, Vinci MC, Pandolfi S, Penachioni JY, Montagnani V, Olivito B, Gattai R, Pimpinelli N, Gerlini G, Borgognoni L, et al: Hedgehog-GLI signaling drives self-renewal and tumorigenicity of human melanoma-initiating cells. Stem Cells. 30:1808–1818. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Davies MA and Kopetz S: Overcoming resistance to MAPK pathway inhibitors. J Natl Cancer Inst. 105:9–10. 2013. View Article : Google Scholar

24 

Obenauf AC, Zou Y, Ji AL, Vanharanta S, Shu W, Shi H, Kong X, Bosenberg MC, Wiesner T, Rosen N, et al: Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 520:368–372. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Vlčková K, Ondrušová L, Vachtenheim J, Réda J, Dundr P, Zadinová M, Žáková P and Poučková P: Survivin, a novel target of the Hedgehog/GLI signaling pathway in human tumor cells. Cell Death Dis. 7:e20482016. View Article : Google Scholar

26 

Vachtenheim J, Sestáková B and Tuhácková Z: Inhibition of MITF transcriptional activity independent of targeting p300/CBP coactivators. Pigment Cell Res. 20:41–51. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Packer LM, East P, Reis-Filho JS and Marais R: Identification of direct transcriptional targets of (V600E)BRAF/MEK signalling in melanoma. Pigment Cell Melanoma Res. 22:785–798. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Herraiz C, Journé F, Ghanem G, Jiménez-Cervantes C and García-Borrón JC: Functional status and relationships of melanocortin 1 receptor signaling to the cAMP and extracellular signal-regulated protein kinases 1 and 2 pathways in human melanoma cells. Int J Biochem Cell Biol. 44:2244–2252. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Hao H, Muniz-Medina VM, Mehta H, Thomas NE, Khazak V, Der CJ and Shields JM: Context-dependent roles of mutant B-Raf signaling in melanoma and colorectal carcinoma cell growth. Mol Cancer Ther. 6:2220–2229. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Smalley KS, Lioni M, Dalla Palma M, Xiao M, Desai B, Egyhazi S, Hansson J, Wu H, King AJ, Van Belle P, et al: Increased cyclin D1 expression can mediate BRAF inhibitor resistance in BRAF V600E-mutated melanomas. Mol Cancer Ther. 7:2876–2883. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Singh S, Davis R, Alamanda V, Pireddu R, Pernazza D, Sebti S, Lawrence N and Chellappan S: Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. Mol Cancer Ther. 9:3330–3341. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Domenzain-Reyna C, Hernández D, Miquel-Serra L, Docampo MJ, Badenas C, Fabra A and Bassols A: Structure and regulation of the versican promoter: The versican promoter is regulated by AP-1 and TCF transcription factors in invasive human melanoma cells. J Biol Chem. 284:12306–12317. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Desch P, Asslaber D, Kern D, Schnidar H, Mangelberger D, Alinger B, Stoecher M, Hofbauer SW, Neureiter D, Tinhofer I, et al: Inhibition of GLI, but not Smoothened, induces apoptosis in chronic lymphocytic leukemia cells. Oncogene. 29:4885–4895. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Pan D, Li Y, Li Z, Wang Y, Wang P and Liang Y: Gli inhibitor GANT61 causes apoptosis in myeloid leukemia cells and acts in synergy with rapamycin. Leuk Res. 36:742–748. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Graab U, Hahn H and Fulda S: Identification of a novel synthetic lethality of combined inhibition of hedgehog and PI3K signaling in rhabdomyosarcoma. Oncotarget. 6:8722–8735. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, et al: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 468:968–972. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Nazarian R, Shi H, Wang Q, Kong X, Koya RC, Lee H, Chen Z, Lee MK, Attar N, Sazegar H, et al: Melanomas acquire resistance to B-RAF(V600E) inhibition by RTK or N-RAS upregulation. Nature. 468:973–977. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Poulikakos PI, Persaud Y, Janakiraman M, Kong X, Ng C, Moriceau G, Shi H, Atefi M, Titz B, Gabay MT, et al: RAF inhibitor resistance is mediated by dimerization of aberrantly spliced BRAF(V600E). Nature. 480:387–390. 2011. View Article : Google Scholar : PubMed/NCBI

39 

Haq R, Yokoyama S, Hawryluk EB, Jönsson GB, Frederick DT, McHenry K, Porter D, Tran TN, Love KT, Langer R, et al: BCL2A1 is a lineage-specific antiapoptotic melanoma oncogene that confers resistance to BRAF inhibition. Proc Natl Acad Sci USA. 110:4321–4326. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Zhang DW, Li HY, Lau WY, Cao LQ, Li Y, Jiang XF, Yang XW and Xue P: Gli2 silencing enhances TRAIL-induced apoptosis and reduces tumor growth in human hepatoma cells in vivo. Cancer Biol Ther. 15:1667–1676. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Kumar K, Raza SS, Knab LM, Chow CR, Kwok B, Bentrem DJ, Popovic R, Ebine K, Licht JD and Munshi HG: GLI2-dependent c-MYC upregulation mediates resistance of pancreatic cancer cells to the BET bromodomain inhibitor JQ1. Sci Rep. 5:94892015. View Article : Google Scholar : PubMed/NCBI

42 

Nagao-Kitamoto H, Nagata M, Nagano S, Kitamoto S, Ishidou Y, Yamamoto T, Nakamura S, Tsuru A, Abematsu M, Fujimoto Y, et al: GLI2 is a novel therapeutic target for metastasis of osteosarcoma. Int J Cancer. 136:1276–1284. 2015. View Article : Google Scholar

43 

Soengas MS and Lowe SW: Apoptosis and melanoma chemoresistance. Oncogene. 22:3138–3151. 2003. View Article : Google Scholar : PubMed/NCBI

44 

Caramel J, Papadogeorgakis E, Hill L, Browne GJ, Richard G, Wierinckx A, Saldanha G, Osborne J, Hutchinson P, Tse G, et al: A switch in the expression of embryonic EMT-inducers drives the development of malignant melanoma. Cancer Cell. 24:466–480. 2013. View Article : Google Scholar : PubMed/NCBI

45 

Vandamme N and Berx G: Melanoma cells revive an embryonic transcriptional network to dictate phenotypic heterogeneity. Front Oncol. 4:3522014. View Article : Google Scholar : PubMed/NCBI

46 

Denecker G, Vandamme N, Akay O, Koludrovic D, Taminau J, Lemeire K, Gheldof A, De Craene B, Van Gele M, Brochez L, et al: Identification of a ZEB2-MITF-ZEB1 transcriptional network that controls melanogenesis and melanoma progression. Cell Death Differ. 21:1250–1261. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Vlčková K, Réda J, Ondrušová L, Krayem M, Ghanem G and Vachtenheim J: GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax. Int J Oncol 49: 953-960, 2016.
APA
Vlčková, K., Réda, J., Ondrušová, L., Krayem, M., Ghanem, G., & Vachtenheim, J. (2016). GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax. International Journal of Oncology, 49, 953-960. https://doi.org/10.3892/ijo.2016.3596
MLA
Vlčková, K., Réda, J., Ondrušová, L., Krayem, M., Ghanem, G., Vachtenheim, J."GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax". International Journal of Oncology 49.3 (2016): 953-960.
Chicago
Vlčková, K., Réda, J., Ondrušová, L., Krayem, M., Ghanem, G., Vachtenheim, J."GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax". International Journal of Oncology 49, no. 3 (2016): 953-960. https://doi.org/10.3892/ijo.2016.3596
Copy and paste a formatted citation
x
Spandidos Publications style
Vlčková K, Réda J, Ondrušová L, Krayem M, Ghanem G and Vachtenheim J: GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax. Int J Oncol 49: 953-960, 2016.
APA
Vlčková, K., Réda, J., Ondrušová, L., Krayem, M., Ghanem, G., & Vachtenheim, J. (2016). GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax. International Journal of Oncology, 49, 953-960. https://doi.org/10.3892/ijo.2016.3596
MLA
Vlčková, K., Réda, J., Ondrušová, L., Krayem, M., Ghanem, G., Vachtenheim, J."GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax". International Journal of Oncology 49.3 (2016): 953-960.
Chicago
Vlčková, K., Réda, J., Ondrušová, L., Krayem, M., Ghanem, G., Vachtenheim, J."GLI inhibitor GANT61 kills melanoma cells and acts in synergy with obatoclax". International Journal of Oncology 49, no. 3 (2016): 953-960. https://doi.org/10.3892/ijo.2016.3596
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