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Article

ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo

  • Authors:
    • Qi-Rui Fu
    • Wei Song
    • Yi-Tao Deng
    • Hua-Liang Li
    • Xiao-Mei Mao
    • Chen-Lu Lin
    • Ya-Hui Zheng
    • Shu-Ming Chen
    • Qiong-Hua Chen
    • Qing-Xi Chen
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Cellular Stress Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, P.R. China, College of Life Science and Engineering, Henan University of Urban Construction, Ping Dingshan, Henan 467036, P.R. China, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China
  • Pages: 241-251
    |
    Published online on: November 18, 2016
       https://doi.org/10.3892/ijo.2016.3773
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Abstract

Apoptosis, programmed cell death under physiological or pathological conditions, plays a critical role in the tissue homeostasis of eukaryotes. It is desirable to prevent the occurrence and metastasis of cancer through inducing apoptosis. Our previous study demonstrated that apoptosis could be induced by extract from crocodile in human cholangiocarcinoma. ESC-3, a novel cytotoxic compound isolated from the extract induced apoptosis in Mz-ChA-1 cells via the mitochondria-dependent pathway in a dose-dependent manner. In this study, ESC-3 significantly inhibited the proliferation of A2780 cells and arrested the cells at G2/M phase. After exposure to ESC-3, A2780 cells displayed typical morphological changes and the ability of colony-forming was remarkably inhibited. ESC-3 could significantly upregulate the expression of Bax proteins while Bcl-2 protein remained unchanged, resulting in the elevation of Bax/Bcl-2 ratio, which usually could induce apoptosis. The critical protein of Wnt signaling (β-catenin) was significantly downregulated, whereas Hes1, the downstream protein of Notch signaling, was remarkably attenuated through upregulating the expression of P53. In addition, xenograft models demonstrated that ESC-3 effectively suppressed the growth of OvCa tumors (T/C=42%). Western blot analysis of PCNA and VEGF confirmed that ESC-3 could inhibit the growth and metastasis of OvCa tumors. In conclusion, apoptosis could be induced by ESC-3 through Wnt/β-catenin and Notch signaling in vitro and in vivo, and might have therapeutic potential for the treatment of human OvCa.
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1 

Seidman JD, Horkayne-Szakaly I, Haiba M, Boice CR, Kurman RJ and Ronnett BM: The histologic type and stage distribution of ovarian carcinomas of surface epithelial origin. Int J Gynecol Pathol. 23:41–44. 2004. View Article : Google Scholar

2 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Vaughan S, Coward JI, Bast RC JR, Berchuck A, Berek JS, Brenton JD, Coukos G, Crum CC, Drapkin R, Etemadmoghadam D, et al: Rethinking ovarian cancer: Recommendations for improving outcomes. Nat Rev Cancer. 11:719–725. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Matsuo K, Lin YG, Roman LD and Sood AK: Overcoming platinum resistance in ovarian carcinoma. Expert Opin Investig Drugs. 19:1339–1354. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Wang Y and Morrow JS: Identification and characterization of human SLP-2, a novel homologue of stomatin (band 7.2b) present in erythrocytes and other tissues. J Biol Chem. 275:8062–8071. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Tint GS, Dayal B, Batta AK, Shefer S, Joanen T, McNease L and Salen G: Biliary bile acids, bile alcohols, and sterols of Alligator mississippiensis. J Lipid Res. 21:110–117. 1980.PubMed/NCBI

7 

Yeh YH, Wang DY, Liau MY, Wu ML, Deng JF, Noguchi T and Hwang DF: Bile acid composition in snake bile juice and toxicity of snake bile acids to rats. Comp Biochem Physiol C Toxicol Pharmacol. 136:277–284. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Song W, Li SS, Qiu PP, Shen DY, Tian L, Zhang QY, Liao LX and Chen QX: Apoptosis induced by aqueous extracts of crocodile bile in human heptacarcinoma SMMC-7721. Appl Biochem Biotechnol. 170:15–24. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Song W, Shen DY, Kang JH, Li SS, Zhan HW, Shi Y, Xiong YX, Liang G and Chen QX: Apoptosis of human cholangiocarcinoma cells induced by ESC-3 from Crocodylus siamensis bile. World J Gastroenterol. 18:704–711. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Song W, Tian L, Li SS, Shen DY and Chen QX: The aberrant expression and localization of prohibitin during apoptosis of human cholangiocarcinoma Mz-ChA-1 cells. FEBS Lett. 588:422–428. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Qin F, Song Y, Li Z, Zhao L, Zhang Y and Geng L: S100A8/A9 induces apoptosis and inhibits metastasis of CasKi human cervical cancer cells. Pathol Oncol Res. 16:353–360. 2010. View Article : Google Scholar

12 

Reed JC and Pellecchia M: Apoptosis-based therapies for hematologic malignancies. Blood. 106:408–418. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Stark GR and Taylor WR: Analyzing the G2/M checkpoint. Methods Mol Biol. 280:51–82. 2004.PubMed/NCBI

15 

Danial NN and Korsmeyer SJ: Cell death: Critical control points. Cell. 116:205–219. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Hunot S and Flavell RA: Apoptosis. Death of a monopoly? Science. 292:865–866. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Horowitz NS, Hua J, Powell MA, Gibb RK, Mutch DG and Herzog TJ: Novel cytotoxic agents from an unexpected source: Bile acids and ovarian tumor apoptosis. Gynecol Oncol. 107:344–349. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Yap TA, Carden CP and Kaye SB: Beyond chemotherapy: Targeted therapies in ovarian cancer. Nat Rev Cancer. 9:167–181. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Geurts van Kessel A: The cancer genome: From structure to function. Cell Oncol (Dordr). 37:155–165. 2014. View Article : Google Scholar

20 

Li Y, Wang K, Jiang Y-Z, Chang X-W, Dai C-F and Zheng J: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) inhibits human ovarian cancer cell proliferation. Cell Oncol. 37:429–437. 2014. View Article : Google Scholar

21 

Wang K, Li Y, Jiang YZ, Dai CF, Patankar MS, Song JS and Zheng J: An endogenous aryl hydrocarbon receptor ligand inhibits proliferation and migration of human ovarian cancer cells. Cancer Lett. 340:63–71. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Wang TT and Phang JM: Effects of estrogen on apoptotic pathways in human breast cancer cell line MCF-7. Cancer Res. 55:2487–2489. 1995.PubMed/NCBI

23 

Earnshaw WC, Martins LM and Kaufmann SH: Mammalian caspases: Structure, activation, substrates, and functions during apoptosis. Annu Rev Biochem. 68:383–424. 1999. View Article : Google Scholar

24 

Kang JH, Zhang WQ, Song W, Shen DY, Li SS, Tian L, Shi Y, Liang G, Xiong YX and Chen QX: Apoptosis mechanism of human cholangiocarcinoma cells induced by bile extract from crocodile. Appl Biochem Biotechnol. 166:942–951. 2012. View Article : Google Scholar

25 

Espinoza I and Miele L: Notch inhibitors for cancer treatment. Pharmacol Ther. 139:95–110. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Pannuti A, Foreman K, Rizzo P, Osipo C, Golde T, Osborne B and Miele L: Targeting Notch to target cancer stem cells. Clin Cancer Res. 16:3141–3152. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Koch U and Radtke F: Notch and cancer: A double-edged sword. Cell Mol Life Sci. 64:2746–2762. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Nam Y, Sliz P, Pear WS, Aster JC and Blacklow SC: Cooperative assembly of higher-order Notch complexes functions as a switch to induce transcription. Proc Natl Acad Sci USA. 104:2103–2108. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Nam Y, Sliz P, Song L, Aster JC and Blacklow SC: Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes. Cell. 124:973–983. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Borggrefe T and Oswald F: The Notch signaling pathway: Transcriptional regulation at Notch target genes. Cell Mol Life Sci. 66:1631–1646. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Yun J, Espinoza I, Pannuti A, Romero D, Martinez L, Caskey M, Stanculescu A, Bocchetta M, Rizzo P, Band V, et al: p53 modulates Notch signaling in MCF-7 breast cancer cells by associating with the Notch transcriptional complex via MAML1. J Cell Physiol. 230:3115–3127. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Jeays-Ward K, Hoyle C, Brennan J, Dandonneau M, Alldus G, Capel B and Swain A: Endothelial and steroidogenic cell migration are regulated by WNT4 in the developing mammalian gonad. Development. 130:3663–3670. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Yao HH, Matzuk MM, Jorgez CJ, Menke DB, Page DC, Swain A and Capel B: Follistatin operates downstream of Wnt4 in mammalian ovary organogenesis. Dev Dyn. 230:210–215. 2004. View Article : Google Scholar : PubMed/NCBI

34 

Batheja N, Suriawinata A, Saxena R, Ionescu G, Schwartz M and Thung SN: Expression of p53 and PCNA in cholangiocarcinoma and primary sclerosing cholangitis. Mod Pathol. 13:1265–1268. 2000. View Article : Google Scholar

35 

Ferrara N: Role of vascular endothelial growth factor in regulation of physiological angiogenesis. Am J Physiol Cell Physiol. 280:C1358–C1366. 2001.PubMed/NCBI

36 

Redmer DA, Doraiswamy V, Bortnem BJ, Fisher K, Jablonka-Shariff A, Grazul-Bilska AT and Reynolds LP: Evidence for a role of capillary pericytes in vascular growth of the developing ovine corpus luteum. Biol Reprod. 65:879–889. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fu Q, Song W, Deng Y, Li H, Mao X, Lin C, Zheng Y, Chen S, Chen Q, Chen Q, Chen Q, et al: ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo. Int J Oncol 50: 241-251, 2017.
APA
Fu, Q., Song, W., Deng, Y., Li, H., Mao, X., Lin, C. ... Chen, Q. (2017). ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo. International Journal of Oncology, 50, 241-251. https://doi.org/10.3892/ijo.2016.3773
MLA
Fu, Q., Song, W., Deng, Y., Li, H., Mao, X., Lin, C., Zheng, Y., Chen, S., Chen, Q., Chen, Q."ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo". International Journal of Oncology 50.1 (2017): 241-251.
Chicago
Fu, Q., Song, W., Deng, Y., Li, H., Mao, X., Lin, C., Zheng, Y., Chen, S., Chen, Q., Chen, Q."ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo". International Journal of Oncology 50, no. 1 (2017): 241-251. https://doi.org/10.3892/ijo.2016.3773
Copy and paste a formatted citation
x
Spandidos Publications style
Fu Q, Song W, Deng Y, Li H, Mao X, Lin C, Zheng Y, Chen S, Chen Q, Chen Q, Chen Q, et al: ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo. Int J Oncol 50: 241-251, 2017.
APA
Fu, Q., Song, W., Deng, Y., Li, H., Mao, X., Lin, C. ... Chen, Q. (2017). ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo. International Journal of Oncology, 50, 241-251. https://doi.org/10.3892/ijo.2016.3773
MLA
Fu, Q., Song, W., Deng, Y., Li, H., Mao, X., Lin, C., Zheng, Y., Chen, S., Chen, Q., Chen, Q."ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo". International Journal of Oncology 50.1 (2017): 241-251.
Chicago
Fu, Q., Song, W., Deng, Y., Li, H., Mao, X., Lin, C., Zheng, Y., Chen, S., Chen, Q., Chen, Q."ESC-3 induces apoptosis of human ovarian carcinomas through Wnt/β-catenin and Notch signaling in vitro and in vivo". International Journal of Oncology 50, no. 1 (2017): 241-251. https://doi.org/10.3892/ijo.2016.3773
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