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Article

The expression and function of epithelial membrane protein 1 in laryngeal carcinoma

  • Authors:
    • Hong Li
    • Xiaowen Zhang
    • Xuejun Jiang
    • Xu Ji
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology Head and Neck Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China, Department of Genetics, Teaching and Learning Office, China Medical University, Shenyang, Liaoning, P.R. China, Department of Otolaryngology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 141-148
    |
    Published online on: November 28, 2016
       https://doi.org/10.3892/ijo.2016.3782
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Abstract

In this study, we compared the expression of epithelial membrane protein 1 (EMP1) on the steady-state mRNA level (by quantitative real-time PCR) and on the protein level (by western immunoblot and immunohistochemistry) in 51 pairs of laryngeal carcinoma tissues and matched cancer-free peritumor tissues, and we analyzed the correlation between EMP1 expression and different clinicopathological factors. Furthermore, we ectopically expressed EMP1 in human laryngeal carcinoma Hep-2 cells and examined the effects on cell viability, apoptosis, colonogenicity, and motility, by MTT assay, flow cytometry, colony formation assay and Transwell migration assay, respectively. EMP1 expression (on both the mRNA and protein levels) was significantly lower in the cancer tissues than in matched peritumor tissues (P<0.05). In laryngeal cancers, the level of EMP1 protein was correlated with histological grade (P<0.05), but not with age, gender, clinical stage, cancer subtype or lymph node metastasis (P>0.05). Functionally, ectopic expression of EMP1 in Hep-2 cells significantly reduced cell viability, colony formation, and migration, but enhanced apoptosis. Therefore, EMP1 is a tumor suppressor in laryngeal carcinoma. Boosting EMP1 expression in laryngeal carcinoma initiates multiple anticancer phenotypes and thus presents a promising therapeutic strategy for laryngeal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Zhang X, Jiang X and Ji X: The expression and function of epithelial membrane protein 1 in laryngeal carcinoma. Int J Oncol 50: 141-148, 2017.
APA
Li, H., Zhang, X., Jiang, X., & Ji, X. (2017). The expression and function of epithelial membrane protein 1 in laryngeal carcinoma. International Journal of Oncology, 50, 141-148. https://doi.org/10.3892/ijo.2016.3782
MLA
Li, H., Zhang, X., Jiang, X., Ji, X."The expression and function of epithelial membrane protein 1 in laryngeal carcinoma". International Journal of Oncology 50.1 (2017): 141-148.
Chicago
Li, H., Zhang, X., Jiang, X., Ji, X."The expression and function of epithelial membrane protein 1 in laryngeal carcinoma". International Journal of Oncology 50, no. 1 (2017): 141-148. https://doi.org/10.3892/ijo.2016.3782
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Zhang X, Jiang X and Ji X: The expression and function of epithelial membrane protein 1 in laryngeal carcinoma. Int J Oncol 50: 141-148, 2017.
APA
Li, H., Zhang, X., Jiang, X., & Ji, X. (2017). The expression and function of epithelial membrane protein 1 in laryngeal carcinoma. International Journal of Oncology, 50, 141-148. https://doi.org/10.3892/ijo.2016.3782
MLA
Li, H., Zhang, X., Jiang, X., Ji, X."The expression and function of epithelial membrane protein 1 in laryngeal carcinoma". International Journal of Oncology 50.1 (2017): 141-148.
Chicago
Li, H., Zhang, X., Jiang, X., Ji, X."The expression and function of epithelial membrane protein 1 in laryngeal carcinoma". International Journal of Oncology 50, no. 1 (2017): 141-148. https://doi.org/10.3892/ijo.2016.3782
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