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Article

Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro

  • Authors:
    • Lei Song
    • Ping Duan
    • Yibo Gan
    • Pei Li
    • Chen Zhao
    • Jianzhong Xu
    • Zehua Zhang
    • Qiang Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, First Affliated Hospital, Third Military Medical University, Chongqing 400038, P.R. China, Southwest Eye Hospital, First Affliated Hospital, Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 535-544
    |
    Published online on: December 28, 2016
       https://doi.org/10.3892/ijo.2016.3820
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Abstract

Cisplatin-resistance has become a major impediment in the medical treatment of cancers such as osteosarcoma, the most common primary malignancy of bone. Since lysophosphatidic acid acyltransferase β (LPAATβ) was reported to be critically involved in osteosarcoma, our study investigated the role of LPAATβ in human osteosarcoma with cisplatin-resistance. Expression of LPAATβ or other relevant proteins were analyzed in 40 osteosarcoma patients by immunohistochemistry analysis (IHC), and in cisplatin‑resistant sublines by real-time PCR and western blotting. Next, the synthesized siRNA was inserted into the lentivirus vector and silencing of LPAATβ expression was employed to determine the effect of LPAATβ on cisplatin-resistant osteosarcoma cell viability in vitro and osteosarcoma tumor growth in vivo with cisplatin treatment. Exogenous LPAATβ mediated by heritable RNAi decreased cisplatin‑resistant sensitivity through activating the PI3K/Akt/mTOR signaling pathway. We further demonstrate that silencing LPAATβ effectively inhibited tumor growth in nude mice with xenografts of cisplatin‑resistant osteosarcoma cells. IHC assay results showed that PI3K/Akt/mTOR signaling pathway was also involved in this process. Our results suggested that LPAATβ may play an important role in osteosarcoma and silencing LPAATβ may be exploited as a novel therapeutic strategy for the clinical management of cisplatin-resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Song L, Duan P, Gan Y, Li P, Zhao C, Xu J, Zhang Z and Zhou Q: Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro. Int J Oncol 50: 535-544, 2017.
APA
Song, L., Duan, P., Gan, Y., Li, P., Zhao, C., Xu, J. ... Zhou, Q. (2017). Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro. International Journal of Oncology, 50, 535-544. https://doi.org/10.3892/ijo.2016.3820
MLA
Song, L., Duan, P., Gan, Y., Li, P., Zhao, C., Xu, J., Zhang, Z., Zhou, Q."Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro". International Journal of Oncology 50.2 (2017): 535-544.
Chicago
Song, L., Duan, P., Gan, Y., Li, P., Zhao, C., Xu, J., Zhang, Z., Zhou, Q."Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro". International Journal of Oncology 50, no. 2 (2017): 535-544. https://doi.org/10.3892/ijo.2016.3820
Copy and paste a formatted citation
x
Spandidos Publications style
Song L, Duan P, Gan Y, Li P, Zhao C, Xu J, Zhang Z and Zhou Q: Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro. Int J Oncol 50: 535-544, 2017.
APA
Song, L., Duan, P., Gan, Y., Li, P., Zhao, C., Xu, J. ... Zhou, Q. (2017). Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro. International Journal of Oncology, 50, 535-544. https://doi.org/10.3892/ijo.2016.3820
MLA
Song, L., Duan, P., Gan, Y., Li, P., Zhao, C., Xu, J., Zhang, Z., Zhou, Q."Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro". International Journal of Oncology 50.2 (2017): 535-544.
Chicago
Song, L., Duan, P., Gan, Y., Li, P., Zhao, C., Xu, J., Zhang, Z., Zhou, Q."Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro". International Journal of Oncology 50, no. 2 (2017): 535-544. https://doi.org/10.3892/ijo.2016.3820
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