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The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy

  • Authors:
    • Dai Shimizu
    • Mitsuro Kanda
    • Hiroyuki Sugimoto
    • Masahiro Shibata
    • Haruyoshi Tanaka
    • Hideki Takami
    • Naoki Iwata
    • Masamichi Hayashi
    • Chie Tanaka
    • Daisuke Kobayashi
    • Suguru Yamada
    • Goro Nakayama
    • Masahiko Koike
    • Michitaka Fujiwara
    • Tsutomu Fujii
    • Yasuhiro Kodera
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
    Copyright: © Shimizu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 381-386
    |
    Published online on: January 2, 2017
       https://doi.org/10.3892/ijo.2017.3833
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Abstract

The prognosis of advanced hepatocellular carcinoma (HCC) is dismal. Novel molecular targets for diagnosis and therapy is urgently required. This study evaluated expression and functions of the protein arginine methyltransferase 5 (PRMT5) in HCC. Using HCC cell lines, the expression levels of PRMT5 mRNA were determined using the quantitative real-time reverse-transcription polymerase chain reaction, and the effect of a small interfering PRMT5-siRNA on cell phenotype was evaluated. Further, PRMT5 expression was determined in 144 pairs of resected liver tissues to evaluate its clinical significance. Regardless of their differentiated phenotypes, nine HCC cell lines expressed different levels of PRMT5 mRNA. Inhibition of PRMT5 expression significantly decreased the proliferation, invasion, and migration of HCC cell lines. Although the level of PRMT5 mRNA was not influenced by patient's background liver status, it was significantly higher in HCC tissues than in the corresponding noncancerous tissues. High levels of PRMT5 mRNA in HCC tissues were significantly associated with advanced disease stage and adverse prognosis. In conclusion, our results indicate that PRMT5 may act as a putative oncogene in HCC and that the levels of PRMT5 mRNA represent a promising prognostic marker and a potential target of molecular therapy for HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Shimizu D, Kanda M, Sugimoto H, Shibata M, Tanaka H, Takami H, Iwata N, Hayashi M, Tanaka C, Kobayashi D, Kobayashi D, et al: The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy. Int J Oncol 50: 381-386, 2017.
APA
Shimizu, D., Kanda, M., Sugimoto, H., Shibata, M., Tanaka, H., Takami, H. ... Kodera, Y. (2017). The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy. International Journal of Oncology, 50, 381-386. https://doi.org/10.3892/ijo.2017.3833
MLA
Shimizu, D., Kanda, M., Sugimoto, H., Shibata, M., Tanaka, H., Takami, H., Iwata, N., Hayashi, M., Tanaka, C., Kobayashi, D., Yamada, S., Nakayama, G., Koike, M., Fujiwara, M., Fujii, T., Kodera, Y."The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy". International Journal of Oncology 50.2 (2017): 381-386.
Chicago
Shimizu, D., Kanda, M., Sugimoto, H., Shibata, M., Tanaka, H., Takami, H., Iwata, N., Hayashi, M., Tanaka, C., Kobayashi, D., Yamada, S., Nakayama, G., Koike, M., Fujiwara, M., Fujii, T., Kodera, Y."The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy". International Journal of Oncology 50, no. 2 (2017): 381-386. https://doi.org/10.3892/ijo.2017.3833
Copy and paste a formatted citation
x
Spandidos Publications style
Shimizu D, Kanda M, Sugimoto H, Shibata M, Tanaka H, Takami H, Iwata N, Hayashi M, Tanaka C, Kobayashi D, Kobayashi D, et al: The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy. Int J Oncol 50: 381-386, 2017.
APA
Shimizu, D., Kanda, M., Sugimoto, H., Shibata, M., Tanaka, H., Takami, H. ... Kodera, Y. (2017). The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy. International Journal of Oncology, 50, 381-386. https://doi.org/10.3892/ijo.2017.3833
MLA
Shimizu, D., Kanda, M., Sugimoto, H., Shibata, M., Tanaka, H., Takami, H., Iwata, N., Hayashi, M., Tanaka, C., Kobayashi, D., Yamada, S., Nakayama, G., Koike, M., Fujiwara, M., Fujii, T., Kodera, Y."The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy". International Journal of Oncology 50.2 (2017): 381-386.
Chicago
Shimizu, D., Kanda, M., Sugimoto, H., Shibata, M., Tanaka, H., Takami, H., Iwata, N., Hayashi, M., Tanaka, C., Kobayashi, D., Yamada, S., Nakayama, G., Koike, M., Fujiwara, M., Fujii, T., Kodera, Y."The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy". International Journal of Oncology 50, no. 2 (2017): 381-386. https://doi.org/10.3892/ijo.2017.3833
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