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Article

Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines

  • Authors:
    • Ricardo Bonfim-Silva
    • Fernanda Ursoli Ferreira Melo
    • Carolina Hassibe Thomé
    • Kuruvilla Joseph Abraham
    • Fábio Augusto Labre De Souza
    • Fernando Silva Ramalho
    • Hélio Rubens Machado
    • Ricardo Santos De Oliveira
    • Angelo A. Cardoso
    • Dimas Tadeu Covas
    • Aparecida Maria Fontes
  • View Affiliations / Copyright

    Affiliations: Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre 14049-900, Ribeirão Preto, São Paulo, Brazil, Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre 14049-900, Ribeirão Preto, São Paulo, Brazil, Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre 14049-900, Ribeirão Preto, São Paulo, Brazil, Department of Pediatrics, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre 14049-900, Ribeirão Preto, São Paulo, Brazil, Department of Pathology and Legal Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre 14049-900, Ribeirão Preto, São Paulo, Brazil, Division of Pediatric Neurosurgery of the Department of Surgery and Anatomy, University Hospital of Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre 14049-900, Ribeirão Preto, São Paulo, Brazil, Center for Gene Therapy, City of Hope Alpha Stem Cell Clinic, Duarte, CA 91010, USA, Department of Internal Medicine, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre 14049-900, Ribeirão Preto, São Paulo, Brazil
  • Pages: 1929-1940
    |
    Published online on: October 10, 2017
       https://doi.org/10.3892/ijo.2017.4151
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Abstract

Medulloblastoma (MB) is a malignant childhood brain tumor which at molecular level is classified into at least four major subtypes: WNT, SHH, group C and group D differing in response to treatment. Previous studies have associated changes in expression levels and activation of certain HOX genes with MB development. In the present study, we investigate the role of HOX genes in two attributes acquired by tumor cells: migration and proliferation potential, as well as, in vivo tumorigenic potential. We analyzed UW402, UW473, DAOY and ONS-76 human pediatric MB cell lines and cerebellum primary cultures. Two-color microarray-based gene expression analysis was used to identify differentially expressed HOX genes. Among the various HOX genes significantly overexpressed in DAOY and ONS-76 cell lines compared to UW402 and UW473 cell lines, HOXA10 and HOXB4 were selected for further analysis. The expression levels of these HOX genes were validated by real-time PCR. A mouse model was used to study the effect of the HOXA10 and HOXB4 genes on the in vivo tumorigenic potential and the in vitro proliferative and migration potential of MB cell lines. Our results show that the inhibition of HOXA10 in DAOY cell line led to increased in vitro cell migration while in vitro cell proliferation or in vivo tumorigenic potential were unaffected. We also observed that induced expression of HOXB4 in the UW473 cell line significantly reduced in vitro cell proliferation and migration capability of UW473 cells with no effect on the in vivo tumorigenicity. This suggests that HOXA10 plays a role in migration events and the HOXB4 gene is involved in proliferation and migration processes of medulloblastoma cells, however, it appears that these genes are not essential for the tumorigenic process of these cells.
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View References

1 

Gilbertson RJ and Ellison DW: The origins of medulloblastoma subtypes. Annu Rev Pathol. 3:341–365. 2008. View Article : Google Scholar

2 

Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW and Kleihues P: The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol. 114:97–109. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Garrè ML, Cama A, Bagnasco F, Morana G, Giangaspero F, Brisigotti M, Gambini C, Forni M, Rossi A, Haupt R, et al: Medulloblastoma variants: Age-dependent occurrence and relation to Gorlin syndrome - a new clinical perspective. Clin Cancer Res. 15:2463–2471. 2009. View Article : Google Scholar

4 

Ramaswamy V, Remke M, Bouffet E, Bailey S, Clifford SC, Doz F, Kool M, Dufour C, Vassal G, Milde T, et al: Risk stratifi-cation of childhood medulloblastoma in the molecular era: The current consensus. Acta Neuropathol. 131:821–831. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Northcott PA, Dubuc AM, Pfister S and Taylor MD: Molecular subgroups of medulloblastoma. Expert Rev Neurother. 12:871–884. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Northcott PA, Shih DJ, Remke M, Cho YJ, Kool M, Hawkins C, Eberhart CG, Dubuc A, Guettouche T, Cardentey Y, et al: Rapid, reliable, and reproducible molecular sub-grouping of clinical medulloblastoma samples. Acta Neuropathol. 123:615–626. 2012. View Article : Google Scholar :

7 

Gibson P, Tong Y, Robinson G, Thompson MC, Currle DS, Eden C, Kranenburg TA, Hogg T, Poppleton H, Martin J, et al: Subtypes of medulloblastoma have distinct developmental origins. Nature. 468:1095–1099. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Coluccia D, Figuereido C, Isik S, Smith C and Rutka JT: Medulloblastoma: Tumor biology and relevance to treatment and prognosis paradigm. Curr Neurol Neurosci Rep. 16:432016. View Article : Google Scholar : PubMed/NCBI

9 

Khatua S: Evolving molecular era of childhood medulloblastoma: Time to revisit therapy. Future Oncol. 12:107–117. 2016. View Article : Google Scholar

10 

Parsons DW, Li M, Zhang X, Jones S, Leary RJ, Lin JC, Boca SM, Carter H, Samayoa J, Bettegowda C, et al: The genetic landscape of the childhood cancer medulloblastoma. Science. 331:435–439. 2011. View Article : Google Scholar :

11 

Roy DM, Walsh LA and Chan TA: Driver mutations of cancer epigenomes. Protein Cell. 5:265–296. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Schuettengruber B, Martinez AM, Iovino N and Cavalli G: Trithorax group proteins: Switching genes on and keeping them active. Nat Rev Mol Cell Biol. 12:799–814. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Wang VY and Zoghbi HY: Genetic regulation of cerebellar development. Nat Rev Neurosci. 2:484–491. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Ruddle FH: The role of Hox and Dlx gene cluster in evolution and development. Inborn Errors of Development. Epstein CJ, Erickson RP and Wynshaw-Boris A: Oxford University Press; New York: pp. 653–658. 2008

15 

Takahashi Y, Hamada J, Murakawa K, Takada M, Tada M, Nogami I, Hayashi N, Nakamori S, Monden M, Miyamoto M, et al: Expression profiles of 39 HOX genes in normal human adult organs and anaplastic thyroid cancer cell lines by quantitative real-time RT-PCR system. Exp Cell Res. 293:144–153. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Hutlet B, Theys N, Coste C, Ahn MT, Doshishti-Agolli K, Lizen B and Gofflot F: Systematic expression analysis of Hox genes at adulthood reveals novel patterns in the central nervous system. Brain Struct Funct. 221:1223–1243. 2016. View Article : Google Scholar

17 

Mallo M and Alonso CR: The regulation of Hox gene expression during animal development. Development. 140:3951–3963. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Shah N and Sukumar S: The Hox genes and their roles in oncogenesis. Nat Rev Cancer. 10:361–371. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Quinonez SC and Innis JW: Human HOX gene disorders. Mol Genet Metab. 111:4–15. 2014. View Article : Google Scholar

20 

Wu X, Chen H, Parker B, Rubin E, Zhu T, Lee JS, Argani P and Sukumar S: HOXB7, a homeodomain protein, is overexpressed in breast cancer and confers epithelial-mesenchymal transition. Cancer Res. 66:9527–9534. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Liao WT, Jiang D, Yuan J, Cui YM, Shi XW, Chen CM, Bian XW, Deng YJ and Ding YQ: HOXB7 as a prognostic factor and mediator of colorectal cancer progression. Clin Cancer Res. 17:3569–3578. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Wang Y, Dang Y, Liu J and Ouyang X: The function of homeobox genes and lncRNAs in cancer. Oncol Lett. 12:1635–1641. 2016.PubMed/NCBI

23 

Rodrigues MF, Esteves CM, Xavier FC and Nunes FD: Methylation status of homeobox genes in common human cancers. Genomics. 108:185–193. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Costa BM, Smith JS, Chen Y, Chen J, Phillips HS, Aldape KD, Zardo G, Nigro J, James CD, Fridlyand J, et al: Reversing HOXA9 oncogene activation by PI3K inhibition: Epigenetic mechanism and prognostic significance in human glioblastoma. Cancer Res. 70:453–462. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Tabuse M, Ohta S, Ohashi Y, Fukaya R, Misawa A, Yoshida K, Kawase T, Saya H, Thirant C, Chneiweiss H, et al: Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells. Mol Cancer. 10:602011. View Article : Google Scholar : PubMed/NCBI

26 

Se YB, Kim SH, Kim JY, Kim JE, Dho YS, Kim JW, Kim YH, Woo HG, Kim SH, Kang SH, et al: Underexpression of HOXA11 is associated with treatment resistance and poor prognosis in glioblastoma. Cancer Res Treat. 49:387–398. 2017. View Article : Google Scholar :

27 

Munthe S, Sørensen MD, Thomassen M, Burton M, Kruse TA, Lathia JD, Poulsen FR and Kristensen BW: Migrating glioma cells express stem cell markers and give rise to new tumors upon xenografting. J Neurooncol. 130:53–62. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Manohar CF, Salwen HR, Furtado MR and Cohn SL: Up-regulation of HOXC6, HOXD1, and HOXD8 homeobox gene expression in human neuroblastoma cells following chemical induction of differentiation. Tumour Biol. 17:34–47. 1996. View Article : Google Scholar : PubMed/NCBI

29 

Zhang X, Hamada J, Nishimoto A, Takahashi Y, Murai T, Tada M and Moriuchi T: HOXC6 and HOXC11 increase transcription of S100beta gene in BrdU-induced in vitro differentiation of GOTO neuroblastoma cells into Schwannian cells. J Cell Mol Med. 11:299–306. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Chakravadhanula M, Ozols VV, Hampton CN, Zhou L, Catchpoole D and Bhardwaj RD: Expression of the HOX genes and HOTAIR in atypical teratoid rhabdoid tumors and other pediatric brain tumors. Cancer Genet. 207:425–428. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Bodey B, Bodey B Jr, Siegel SE and Kaiser HE: Immunocytochemical detection of the homeobox B3, B4, and C6 gene products in childhood medulloblastomas/primitive neuroectodermal tumors. Anticancer Res. 20:1769–1780. 2000.PubMed/NCBI

32 

Vo BT, Li C, Morgan MA, Theurillat I, Finkelstein D, Wright S, Hyle J, Smith SMC, Fan Y, Wang YD, et al: Inactivation of Ezh2 upregulates Gfi1 and drives aggressive Myc-driven group 3 medulloblastoma. Cell Rep. 18:2907–2917. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Margueron R and Reinberg D: The Polycomb complex PRC2 and its mark in life. Nature. 469:343–349. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Castro-Gamero AM, Borges KS, Lira RC, Andrade AF, Fedatto PF, Cruzeiro GA, Silva RB, Fontes AM, Valera ET, Bobola M, et al: Chromosomal heterogeneity and instability characterize pediatric medulloblastoma cell lines and affect neoplastic phenotype. Cytotechnology. 65:871–885. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Jacobsen PF, Jenkyn DJ and Papadimitriou JM: Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice. J Neuropathol Exp Neurol. 44:472–485. 1985. View Article : Google Scholar : PubMed/NCBI

37 

Yamada M, Shimizu K, Tamura K, Okamoto Y, Matsui Y, Moriuchi S, Park K, Mabuchi E, Yamamoto K, Hayakawa T, et al: Establishment and biological characterization of human medulloblastoma cell lines. No To Shinkei. 41:695–702. 1989.In Japanese. PubMed/NCBI

38 

Ivanov DP, Coyle B, Walker DA and Grabowska AM: In vitro models of medulloblastoma: Choosing the right tool for the job. J Biotechnol. 236:10–25. 2016. View Article : Google Scholar : PubMed/NCBI

39 

de Oliveira GP, Alves CJ and Chadi G: Early gene expression changes in spinal cord from SOD1G93A Amyotrophic Lateral Sclerosis animal model. Front Cell Neurosci. 7:2162013.

40 

Covas DT, Panepucci RA, Fontes AM, Silva WA Jr, Orellana MD, Freitas MC, Neder L, Santos AR, Peres LC, Jamur MC, et al: Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts. Exp Hematol. 36:642–654. 2008. View Article : Google Scholar : PubMed/NCBI

41 

Fontes AM, Melo FU, Greene LJ, Faça VM, Lin Y, Gerson SL and Covas DT: Production of human factor VIII-FL in 293T cells using the bicistronic MGMT(P140K)-retroviral vector. Genet Mol Res. 11:775–789. 2012. View Article : Google Scholar : PubMed/NCBI

42 

Albesiano E, Messmer BT, Damle RN, Allen SL, Rai KR and Chiorazzi N: Activation-induced cytidine deaminase in chronic lymphocytic leukemia B cells: Expression as multiple forms in a dynamic, variably sized fraction of the clone. Blood. 102:3333–3339. 2003. View Article : Google Scholar : PubMed/NCBI

43 

Ferguson RE, Carroll HP, Harris A, Maher ER, Selby PJ and Banks RE: Housekeeping proteins: A preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics. 5:566–571. 2005. View Article : Google Scholar : PubMed/NCBI

44 

Thomé CH, dos Santos GA, Ferreira GA, Scheucher PS, Izumi C, Leopoldino AM, Simão AM, Ciancaglini P, de Oliveira KT, Chin A, et al: Linker for activation of T-cell family member2 (LAT2) a lipid raft adaptor protein for AKT signaling, is an early mediator of alkylphospholipid anti-leukemic activity. Mol Cell Proteomics. 11:1898–1912. 2012. View Article : Google Scholar : PubMed/NCBI

45 

Palma CS, Grassi ML, Thomé CH, Ferreira GA, Albuquerque D, Pinto MT, Ferreira Melo FU, Kashima S, Covas DT, Pitteri SJ, et al: Proteomic analysis of epithelial to mesenchymal transition (EMT) reveals cross-talk between SNAIL and HDAC1 proteins in breast cancer cells. Mol Cell Proteomics. 15:906–917. 2016. View Article : Google Scholar :

46 

Triscott J, Lee C, Foster C, Manoranjan B, Pambid MR, Berns R, Fotovati A, Venugopal C, O'Halloran K, Narendran A, et al: Personalizing the treatment of pediatric medulloblastoma: Polo-like kinase 1 as a molecular target in high-risk children. Cancer Res. 73:6734–6744. 2013. View Article : Google Scholar : PubMed/NCBI

47 

Bobola MS, Silber JR, Ellenbogen RG, Geyer JR, Blank A and Goff RD: O6-methylguanine-DNA methyltransferase, O6-benzylguanine, and resistance to clinical alkylators in pediatric primary brain tumor cell lines. Clin Cancer Res. 11:2747–2755. 2005. View Article : Google Scholar : PubMed/NCBI

48 

Murat A, Migliavacca E, Gorlia T, Lambiv WL, Shay T, Hamou MF, de Tribolet N, Regli L, Wick W, Kouwenhoven MC, et al: Stem cell-related 'self-renewal' signature and high epidermal growth factor receptor expression associated with resistance to concomitant chemoradiotherapy in glioblastoma. J Clin Oncol. 26:3015–3024. 2008. View Article : Google Scholar : PubMed/NCBI

49 

Kurscheid S, Bady P, Sciuscio D, Samarzija I, Shay T, Vassallo I, Criekinge WV, Daniel RT, van den Bent MJ, Marosi C, et al: Chromosome 7 gain and DNA hypermethylation at the HOXA10 locus are associated with expression of a stem cell related HOX-signature in glioblastoma. Genome Biol. 16:162015. View Article : Google Scholar : PubMed/NCBI

50 

Perkins EJ, Bao W, Guan X, Ang CY, Wolfinger RD, Chu TM, Meyer SA and Inouye LS: Comparison of transcriptional responses in liver tissue and primary hepatocyte cell cultures after exposure to hexahydro-1,3,5-trinitro-1,3,5-triazine. BMC Bioinformatics. 7(Suppl 4): S222006. View Article : Google Scholar

51 

Snuderl M, Batista A, Kirkpatrick ND, Ruiz de Almodovar C, Riedemann L, Walsh EC, Anolik R, Huang Y, Martin JD, Kamoun W, et al: Targeting placental growth factor/neuropilin 1 pathway inhibits growth and spread of medulloblastoma. Cell. 152:1065–1076. 2013. View Article : Google Scholar : PubMed/NCBI

52 

Sengupta S, Weeraratne SD, Sun H, Phallen J, Rallapalli SK, Teider N, Kosaras B, Amani V, Pierre-Francois J, Tang Y, et al: α5-GABAA receptors negatively regulate MYC-amplified medulloblastoma growth. Acta Neuropathol. 127:593–603. 2014. View Article : Google Scholar

53 

Tang W, Jiang Y, Mu X, Xu L, Cheng W and Wang X: MiR-135a functions as a tumor suppressor in epithelial ovarian cancer and regulates HOXA10 expression. Cell Signal. 26:1420–1426. 2014. View Article : Google Scholar : PubMed/NCBI

54 

Hemmesi K, Squadrito ML, Mestdagh P, Conti V, Cominelli M, Piras IS, Sergi LS, Piccinin S, Maestro R, Poliani PL, et al: miR-135a inhibits cancer stem cell-driven medulloblastoma development by directly repressing Arhgef6 expression. Stem Cells. 33:1377–1389. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Bonfim-Silva R, Ferreira Melo FU, Thomé CH, Abraham KJ, De Souza FL, Ramalho FS, Machado HR, De Oliveira RS, Cardoso AA, Covas DT, Covas DT, et al: Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines. Int J Oncol 51: 1929-1940, 2017.
APA
Bonfim-Silva, R., Ferreira Melo, F.U., Thomé, C.H., Abraham, K.J., De Souza, F.L., Ramalho, F.S. ... Fontes, A.M. (2017). Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines. International Journal of Oncology, 51, 1929-1940. https://doi.org/10.3892/ijo.2017.4151
MLA
Bonfim-Silva, R., Ferreira Melo, F. U., Thomé, C. H., Abraham, K. J., De Souza, F. L., Ramalho, F. S., Machado, H. R., De Oliveira, R. S., Cardoso, A. A., Covas, D. T., Fontes, A. M."Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines". International Journal of Oncology 51.6 (2017): 1929-1940.
Chicago
Bonfim-Silva, R., Ferreira Melo, F. U., Thomé, C. H., Abraham, K. J., De Souza, F. L., Ramalho, F. S., Machado, H. R., De Oliveira, R. S., Cardoso, A. A., Covas, D. T., Fontes, A. M."Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines". International Journal of Oncology 51, no. 6 (2017): 1929-1940. https://doi.org/10.3892/ijo.2017.4151
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Spandidos Publications style
Bonfim-Silva R, Ferreira Melo FU, Thomé CH, Abraham KJ, De Souza FL, Ramalho FS, Machado HR, De Oliveira RS, Cardoso AA, Covas DT, Covas DT, et al: Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines. Int J Oncol 51: 1929-1940, 2017.
APA
Bonfim-Silva, R., Ferreira Melo, F.U., Thomé, C.H., Abraham, K.J., De Souza, F.L., Ramalho, F.S. ... Fontes, A.M. (2017). Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines. International Journal of Oncology, 51, 1929-1940. https://doi.org/10.3892/ijo.2017.4151
MLA
Bonfim-Silva, R., Ferreira Melo, F. U., Thomé, C. H., Abraham, K. J., De Souza, F. L., Ramalho, F. S., Machado, H. R., De Oliveira, R. S., Cardoso, A. A., Covas, D. T., Fontes, A. M."Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines". International Journal of Oncology 51.6 (2017): 1929-1940.
Chicago
Bonfim-Silva, R., Ferreira Melo, F. U., Thomé, C. H., Abraham, K. J., De Souza, F. L., Ramalho, F. S., Machado, H. R., De Oliveira, R. S., Cardoso, A. A., Covas, D. T., Fontes, A. M."Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines". International Journal of Oncology 51, no. 6 (2017): 1929-1940. https://doi.org/10.3892/ijo.2017.4151
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