Open Access

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells

  • Authors:
    • Wei Jia
    • Xingjun Jiang
    • Weidong Liu
    • Lei Wang
    • Bin Zhu
    • Hecheng Zhu
    • Xingdong Liu
    • Meizuo Zhong
    • Dan Xie
    • Wei Huang
    • Wenting Jia
    • Shasha Li
    • Xuxu Liu
    • Xiang Zuo
    • Damei Cheng
    • Jianwu Dai
    • Caiping Ren
  • View Affiliations

  • Published online on: March 20, 2018     https://doi.org/10.3892/ijo.2018.4330
  • Pages: 1787-1800
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Three-dimensional (3D) culture has been increasingly used to investigate tumor cell biology for improved simulation of the natural developing environment. However, the way in which 3D culture affects the gene expression and biological functions of glioma cells remains to be fully elucidated. In the present study, 3D culture environments were established using collagen scaffolds with different pore sizes, followed by the comparison of gene expression profiles and associated biological functions of glioma cells, including the U87, U251 and HS683 cell lines, in 3D collagen scaffolds with conventional two-dimensional (2D) cultured cells. Finally, the possible signaling pathways regulating these differences were investigated. It was found that the 3D collagen scaffold culture upregulated the expression of genes associated with stemness, cell cycle, apoptosis, epithelia-mesenchymal transition, migration, invasion and glioma malignancy, and induced the corresponding functional changes. Apoptotic pathways, the Wnt pathway, Sonic Hedgehog pathway and Notch pathway, may be involved in the regulation of these changes. The aperture size of the collagen-scaffold did not appear to affect the gene expression or functions of the glioma cells. The results of the study suggested that the 3D collagen scaffold enhanced the malignancy of glioma cells and may be a promising in vitro platform for investigations of glioma.
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June-2018
Volume 52 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Jia W, Jiang X, Liu W, Wang L, Zhu B, Zhu H, Liu X, Zhong M, Xie D, Huang W, Huang W, et al: Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells. Int J Oncol 52: 1787-1800, 2018
APA
Jia, W., Jiang, X., Liu, W., Wang, L., Zhu, B., Zhu, H. ... Ren, C. (2018). Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells. International Journal of Oncology, 52, 1787-1800. https://doi.org/10.3892/ijo.2018.4330
MLA
Jia, W., Jiang, X., Liu, W., Wang, L., Zhu, B., Zhu, H., Liu, X., Zhong, M., Xie, D., Huang, W., Jia, W., Li, S., Liu, X., Zuo, X., Cheng, D., Dai, J., Ren, C."Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells". International Journal of Oncology 52.6 (2018): 1787-1800.
Chicago
Jia, W., Jiang, X., Liu, W., Wang, L., Zhu, B., Zhu, H., Liu, X., Zhong, M., Xie, D., Huang, W., Jia, W., Li, S., Liu, X., Zuo, X., Cheng, D., Dai, J., Ren, C."Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells". International Journal of Oncology 52, no. 6 (2018): 1787-1800. https://doi.org/10.3892/ijo.2018.4330