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S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells

  • Authors:
    • Lu Ding
    • Chengwei Wang
    • Yong Cui
    • Xiaoping Han
    • Yang Zhou
    • Jingping Bai
    • Rong Li
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    Affiliations: Department of Orthopedics, Fifth Affiliated Hospital, Xinjiang Medical , Urumqi, Xinjiang 830011, P.R. China, Department of Orthopedics, Sixth Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830002, P.R. China, Department of Orthopedics, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Maternal, Child and Adolescent Health, College of Public Health, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1841-1852
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    Published online on: March 29, 2018
       https://doi.org/10.3892/ijo.2018.4345
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Abstract

Osteosarcoma (OS), a common worldwide primary aggressive bone malignancy, arises from primitive transformed cells of mesenchymal origin and usually attacks adolescents and young adults. Methotrexate (MTX) is the anti-folate drug used as a pivotal chemotherapeutic agent in the treatment of OS. However, patients with OS often develop drug resistance, leading to poor treatment outcomes. In the present study, in order to explore the underlying mechanisms responsible for MTX resistance, we established MTX‑resistant OS cells using the U2OS and MG63 cell lines and examined whether MTX‑resistant OS cells underwent epithelial-mesenchymal transition (EMT) by Transwell assay, wound healing assay, MTT assay, RT-PCR and western blot analysis. We found that the viability of the MTX‑resistant cells remained relatively unaltered following further treatment with MTX compared to the parental cells. The resistant cells appeared to possess a mesenchymal phenotype, with an elongated and more spindle‑like shape, and acquired enhanced invasive, migratory and attachment abilities. The measurement of EMT markers also supported EMT transition in the MTX‑resistant OS cells. Our result further demonstrated that the overexpression of S-phase kinase-associated protein 2 (Skp2) was closely involved in the resistance of OS cells to MTX and in the acquirement of EMT properties. Thus, the pharmacological inhibition of Skp2 may prove to be a novel therapeutic strategy with which to overcome drug resistance in OS.
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Spandidos Publications style
Ding L, Wang C, Cui Y, Han X, Zhou Y, Bai J and Li R: S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells. Int J Oncol 52: 1841-1852, 2018.
APA
Ding, L., Wang, C., Cui, Y., Han, X., Zhou, Y., Bai, J., & Li, R. (2018). S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells. International Journal of Oncology, 52, 1841-1852. https://doi.org/10.3892/ijo.2018.4345
MLA
Ding, L., Wang, C., Cui, Y., Han, X., Zhou, Y., Bai, J., Li, R."S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells". International Journal of Oncology 52.6 (2018): 1841-1852.
Chicago
Ding, L., Wang, C., Cui, Y., Han, X., Zhou, Y., Bai, J., Li, R."S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells". International Journal of Oncology 52, no. 6 (2018): 1841-1852. https://doi.org/10.3892/ijo.2018.4345
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Spandidos Publications style
Ding L, Wang C, Cui Y, Han X, Zhou Y, Bai J and Li R: S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells. Int J Oncol 52: 1841-1852, 2018.
APA
Ding, L., Wang, C., Cui, Y., Han, X., Zhou, Y., Bai, J., & Li, R. (2018). S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells. International Journal of Oncology, 52, 1841-1852. https://doi.org/10.3892/ijo.2018.4345
MLA
Ding, L., Wang, C., Cui, Y., Han, X., Zhou, Y., Bai, J., Li, R."S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells". International Journal of Oncology 52.6 (2018): 1841-1852.
Chicago
Ding, L., Wang, C., Cui, Y., Han, X., Zhou, Y., Bai, J., Li, R."S-phase kinase-associated protein 2 is involved in epithelial-mesenchymal transition in methotrexate-resistant osteosarcoma cells". International Journal of Oncology 52, no. 6 (2018): 1841-1852. https://doi.org/10.3892/ijo.2018.4345
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