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Article

Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer

  • Authors:
    • Zhuan-Peng Chen
    • Jian-Chang Wei
    • Qiang Wang
    • Ping Yang
    • Wang-Lin Li
    • Feng He
    • Hua‑Cui Chen
    • He Hu
    • Jun-Bin Zhong
    • Jie Cao
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou Guangdong 510180, P.R. China
  • Pages: 1227-1236
    |
    Published online on: June 22, 2018
       https://doi.org/10.3892/ijo.2018.4451
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Abstract

Colorectal cancer (CRC) is the third most common type of cancer; however, the molecular mechanisms underlying colorectal tumor metastasis and growth remain elusive. Recently, accumulating evidence has indicated that long non‑coding RNAs (lncRNAs) play a critical role in CRC progression and metastasis; however, the biological role and clinical significance of lncRNA 00152 (lnc00152) in CRC remains largely unknown. Thus, in this study, lnc00152 expression was measured in 80 human CRC tissue samples, 40 non‑cancerous tissue samples, and 3 CRC cell lines (SW480, SW620 and LoVo) using RT‑qPCR. We examined the effects of lnc00152 on CRC cells following transfection with lnc00152 overexpression plasmid or respective siRNA in vitro and in vivo. Luciferase assays revealed the mechanism driving competitive endogenous RNA (ceRNA). We identified that lnc00152 was aberrantly overexpressed in colorectal tumors and cancer cells and that lnc00152 was modulated by miRNA‑206. lnc00152 overexpression enhanced the proliferative and invasive ability of CRC cells in vitro, promoted tumor growth in vivo, and was associated with the shorter overall survival of patients with CRC. In addition, lnc00152 overexpression promoted epithelial-mesenchymal transition (EMT) and increased neuropilin‑1 (NRP1) expression in the CRC cells. By contrast, lnc00152 silencing exerted a counteractive effect. Collectively, these findings demonstrate the critical role of lnc00152 in tumor growth and progression in CRC, and identify a novel therapeutic target associated with CRC development and progression.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Z, Wei J, Wang Q, Yang P, Li W, He F, Chen HC, Hu H, Zhong J, Cao J, Cao J, et al: Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer. Int J Oncol 53: 1227-1236, 2018.
APA
Chen, Z., Wei, J., Wang, Q., Yang, P., Li, W., He, F. ... Cao, J. (2018). Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer. International Journal of Oncology, 53, 1227-1236. https://doi.org/10.3892/ijo.2018.4451
MLA
Chen, Z., Wei, J., Wang, Q., Yang, P., Li, W., He, F., Chen, H., Hu, H., Zhong, J., Cao, J."Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer". International Journal of Oncology 53.3 (2018): 1227-1236.
Chicago
Chen, Z., Wei, J., Wang, Q., Yang, P., Li, W., He, F., Chen, H., Hu, H., Zhong, J., Cao, J."Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer". International Journal of Oncology 53, no. 3 (2018): 1227-1236. https://doi.org/10.3892/ijo.2018.4451
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Z, Wei J, Wang Q, Yang P, Li W, He F, Chen HC, Hu H, Zhong J, Cao J, Cao J, et al: Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer. Int J Oncol 53: 1227-1236, 2018.
APA
Chen, Z., Wei, J., Wang, Q., Yang, P., Li, W., He, F. ... Cao, J. (2018). Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer. International Journal of Oncology, 53, 1227-1236. https://doi.org/10.3892/ijo.2018.4451
MLA
Chen, Z., Wei, J., Wang, Q., Yang, P., Li, W., He, F., Chen, H., Hu, H., Zhong, J., Cao, J."Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer". International Journal of Oncology 53.3 (2018): 1227-1236.
Chicago
Chen, Z., Wei, J., Wang, Q., Yang, P., Li, W., He, F., Chen, H., Hu, H., Zhong, J., Cao, J."Long non‑coding RNA 00152 functions as a competing endogenous RNA to regulate NRP1 expression by sponging with miRNA‑206 in colorectal cancer". International Journal of Oncology 53, no. 3 (2018): 1227-1236. https://doi.org/10.3892/ijo.2018.4451
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