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Article

TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma

  • Authors:
    • Yi Qin
    • Jichao  Ye
    • Fulan Zhao
    • Shaoyu Hu
    • Suwei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Zhuhai Hospital, Jinan University, Zhuhai People's Hospital, Zhuhai, Guangdong 519000, P.R. China, Department of Orthopedics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. China
  • Pages: 1643-1656
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    Published online on: July 20, 2018
       https://doi.org/10.3892/ijo.2018.4494
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Abstract

The present study aimed to investigate candidate genes involved in the development and metastasis of osteosarcoma. Candidate genes were screened preliminarily from the Gene Expression Omnibus database and then validated using actual tumor tissues collected from patients with osteosarcoma. The cells were prepared and transfected with specific gene-targeted small interfering RNA followed by an MTS assay for cell viability detection and Transwell assays for cell migration and invasion capacity detection. The cell apoptosis was determined by flow cytometry and the protein level of the genes was detected by western blot analysis. An in vivo nude model was used and injected with cells to detect the functions of the genes. Transcriptome sequencing was performed to verify the regulation network, followed by reverse transcription-quantitative polymerase chain reaction and western blot analyses for validation. Increased tripartite motif-containing protein 2 (TRIM2) was detected in the osteosarcoma tumor tissues compared with normal tissues. The inhibition of TRIM2 induced lower cell viability and cell invasion capacity, and increased the rate of cell apoptosis. Decreased TRIM2 also inhibited the development and metastasis of osteosarcoma in the nude mouse models. The transcriptome sequencing revealed that the regulation of TRIM2 may be correlated with genes, Sirtuin 4, DNA damage inducible transcript 3, cAMP responsive element binding protein 5, G protein-coupled receptor 65 (GPR65) and ADP-ribosyltransferase 5. Western blot analysis indicated that TRIM2 regulated the development and metastasis of osteosarcoma via the phosphoinositide 3-kinase/protein kinase B signaling pathway. Therefore, TRIM2 performs important functions in regulating the development and metastasis of osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Qin Y, Ye J, Zhao F, Hu S and Wang S: TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma. Int J Oncol 53: 1643-1656, 2018.
APA
Qin, Y., Ye, J., Zhao, F., Hu, S., & Wang, S. (2018). TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma. International Journal of Oncology, 53, 1643-1656. https://doi.org/10.3892/ijo.2018.4494
MLA
Qin, Y., Ye, J., Zhao, F., Hu, S., Wang, S."TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma". International Journal of Oncology 53.4 (2018): 1643-1656.
Chicago
Qin, Y., Ye, J., Zhao, F., Hu, S., Wang, S."TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma". International Journal of Oncology 53, no. 4 (2018): 1643-1656. https://doi.org/10.3892/ijo.2018.4494
Copy and paste a formatted citation
x
Spandidos Publications style
Qin Y, Ye J, Zhao F, Hu S and Wang S: TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma. Int J Oncol 53: 1643-1656, 2018.
APA
Qin, Y., Ye, J., Zhao, F., Hu, S., & Wang, S. (2018). TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma. International Journal of Oncology, 53, 1643-1656. https://doi.org/10.3892/ijo.2018.4494
MLA
Qin, Y., Ye, J., Zhao, F., Hu, S., Wang, S."TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma". International Journal of Oncology 53.4 (2018): 1643-1656.
Chicago
Qin, Y., Ye, J., Zhao, F., Hu, S., Wang, S."TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma". International Journal of Oncology 53, no. 4 (2018): 1643-1656. https://doi.org/10.3892/ijo.2018.4494
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