Open Access

Potential ceRNA networks involved in autophagy suppression of pancreatic cancer caused by chloroquine diphosphate: A study based on differentially‑expressed circRNAs, lncRNAs, miRNAs and mRNAs

  • Authors:
    • Dan‑Ming Wei
    • Meng‑Tong Jiang
    • Peng Lin
    • Hong Yang
    • Yi‑Wu Dang
    • Qiao Yu
    • Dan‑Yu Liao
    • Dian‑Zhong Luo
    • Gang Chen
  • View Affiliations

  • Published online on: December 10, 2018     https://doi.org/10.3892/ijo.2018.4660
  • Pages: 600-626
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Autophagy has been reported to be involved in the occurrence and development of pancreatic cancer. However, the mechanism of autophagy‑associated non‑coding RNAs (ncRNAs) in pancreatic cancer remains largely unknown. In the present study, microarrays were used to detect differential expression of mRNAs, microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs) post autophagy suppression by chloroquine diphosphate in PANC‑1 cells. Collectively, 3,966 mRNAs, 3,184 lncRNAs and 9,420 circRNAs were differentially expressed. Additionally, only two miRNAs (hsa‑miR‑663a‑5p and hsa‑miR‑154‑3p) were underexpressed in the PANC‑1 cells in the autophagy‑suppression group. Furthermore, miR‑663a‑5p with 9 circRNAs, 8 lncRNAs and 46 genes could form a prospective ceRNA network associated with autophagy in pancreatic cancer cells. In addition, another ceRNA network containing miR‑154‑3p, 5 circRNAs, 2 lncRNAs and 11 genes was also constructed. The potential multiple ceRNA, miRNA and mRNA associations may serve pivotal roles in the autophagy of pancreatic cancer cells, which lays the theoretical foundation for subsequent investigations on pancreatic cancer.
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February-2019
Volume 54 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Wei DM, Jiang MT, Lin P, Yang H, Dang YW, Yu Q, Liao DY, Luo DZ and Chen G: Potential ceRNA networks involved in autophagy suppression of pancreatic cancer caused by chloroquine diphosphate: A study based on differentially‑expressed circRNAs, lncRNAs, miRNAs and mRNAs. Int J Oncol 54: 600-626, 2019
APA
Wei, D., Jiang, M., Lin, P., Yang, H., Dang, Y., Yu, Q. ... Chen, G. (2019). Potential ceRNA networks involved in autophagy suppression of pancreatic cancer caused by chloroquine diphosphate: A study based on differentially‑expressed circRNAs, lncRNAs, miRNAs and mRNAs. International Journal of Oncology, 54, 600-626. https://doi.org/10.3892/ijo.2018.4660
MLA
Wei, D., Jiang, M., Lin, P., Yang, H., Dang, Y., Yu, Q., Liao, D., Luo, D., Chen, G."Potential ceRNA networks involved in autophagy suppression of pancreatic cancer caused by chloroquine diphosphate: A study based on differentially‑expressed circRNAs, lncRNAs, miRNAs and mRNAs". International Journal of Oncology 54.2 (2019): 600-626.
Chicago
Wei, D., Jiang, M., Lin, P., Yang, H., Dang, Y., Yu, Q., Liao, D., Luo, D., Chen, G."Potential ceRNA networks involved in autophagy suppression of pancreatic cancer caused by chloroquine diphosphate: A study based on differentially‑expressed circRNAs, lncRNAs, miRNAs and mRNAs". International Journal of Oncology 54, no. 2 (2019): 600-626. https://doi.org/10.3892/ijo.2018.4660