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Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps

  • Authors:
    • Yan Li
    • Yonglin Yang
    • Tingting Gan
    • Jiawei Zhou
    • Fan Hu
    • Nannan Hao
    • Baorui Yuan
    • Yu Chen
    • Mingshun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Infectious Disease, Nanjing Medical University Nanjing First Hospital, Nanjing, Jiangsu 210006, P.R. China, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210016, P.R. China, Key Laboratory of Antibody Technique of Health Ministry, Nanjing Medical University, Nanjing, Jiangsu 210016, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 69-80
    |
    Published online on: May 21, 2019
       https://doi.org/10.3892/ijo.2019.4808
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Abstract

Neutrophil infiltration is frequently observed in lung cancer tissues. Extracellular RNAs (exRNAs) may facilitate tumor progression. The present study investigated the cross‑talk of tumor exRNAs and neutrophil extracellular traps (NETs) in lung cancer. Lewis lung carcinoma (LLC) cells were cultured with the deprived sera. And the cell culture supernatants (CCS) were analyzed in vitro and in vivo. The results revealed that exRNAs from lung cancer CCS promoted the inflammatory cytokine interleukin‑1β and reduced the vascular cell adhesion molecule‑1 expression in lung epithelial cells. Lung cancer CCS‑treated epithelial cells induced the production of NETs. By contrast, NETs reduced the tight junction protein claudin‑5 in epithelial cells. Furthermore, NETs caused the necrosis of epithelial cells, which resulted in the release of exRNAs. In mice, lung cancer cells instilled in the lung recruited neutrophils and initiated NETs. In patients with lung cancer, NETs were also observed. These results suggested that exRNAs in the cell culture supernatant may indirectly induce NETs and contribute to lung cancer oncogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Yang Y, Gan T, Zhou J, Hu F, Hao N, Yuan B, Chen Y and Zhang M: Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps. Int J Oncol 55: 69-80, 2019.
APA
Li, Y., Yang, Y., Gan, T., Zhou, J., Hu, F., Hao, N. ... Zhang, M. (2019). Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps. International Journal of Oncology, 55, 69-80. https://doi.org/10.3892/ijo.2019.4808
MLA
Li, Y., Yang, Y., Gan, T., Zhou, J., Hu, F., Hao, N., Yuan, B., Chen, Y., Zhang, M."Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps". International Journal of Oncology 55.1 (2019): 69-80.
Chicago
Li, Y., Yang, Y., Gan, T., Zhou, J., Hu, F., Hao, N., Yuan, B., Chen, Y., Zhang, M."Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps". International Journal of Oncology 55, no. 1 (2019): 69-80. https://doi.org/10.3892/ijo.2019.4808
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Yang Y, Gan T, Zhou J, Hu F, Hao N, Yuan B, Chen Y and Zhang M: Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps. Int J Oncol 55: 69-80, 2019.
APA
Li, Y., Yang, Y., Gan, T., Zhou, J., Hu, F., Hao, N. ... Zhang, M. (2019). Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps. International Journal of Oncology, 55, 69-80. https://doi.org/10.3892/ijo.2019.4808
MLA
Li, Y., Yang, Y., Gan, T., Zhou, J., Hu, F., Hao, N., Yuan, B., Chen, Y., Zhang, M."Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps". International Journal of Oncology 55.1 (2019): 69-80.
Chicago
Li, Y., Yang, Y., Gan, T., Zhou, J., Hu, F., Hao, N., Yuan, B., Chen, Y., Zhang, M."Extracellular RNAs from lung cancer cells activate epithelial cells and induce neutrophil extracellular traps". International Journal of Oncology 55, no. 1 (2019): 69-80. https://doi.org/10.3892/ijo.2019.4808
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