Open Access

MicroRNA‑301a/ZNRF3/wnt/β‑catenin signal regulatory crosstalk mediates glioma progression

  • Authors:
    • Jikui Sun
    • Quanfeng Ma
    • Chang Shu
    • Jinbiao Xiong
    • Banban Li
    • Jingchao Wu
    • Shusheng Zhang
    • Jialin Li
    • Jun Liu
    • Jinhuan Wang
  • View Affiliations

  • Published online on: November 10, 2020     https://doi.org/10.3892/ijo.2020.5145
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNA (miR)‑mediated mRNA and multiple signaling pathway dysregulations have been extensively implicated in several cancer types, including gliomas. Although previous studies have reported that miR‑301a acts as an oncogene, the underlying mechanisms of miR‑301a in the initiation and progression of glioma remain unknown. The present study aimed to investigate the involvement of miR‑301a‑mediated signaling pathway dysregulation in glioma. The results identified that miR‑301a was significantly upregulated in gliomas and was associated with a poor prognosis based on The Cancer Genome Atlas and Chinese Glioma Genome Atlas databases. Moreover, zinc and ring finger 3 (ZNRF3) exerted a critical role in the miR‑301a‑mediated effects on the malignant phenotype, such as by affecting proliferation and apoptosis. Mechanistically, the TOP/FOP luciferase assay, western blotting and immunofluorescence results demonstrated that miR‑301a knockdown inhibited the wnt/β‑catenin signaling pathway, at least partially via ZNRF3, while ZNRF3 was a direct functional target of miR‑301a, as indicated by luciferase reporter assay and western blot analysis. Furthermore, ZNRF3 could in turn repress miR‑301a expression, which was dependent on the wnt pathway. Collectively, the present study identified a novel miR‑301a/ZNRF3/wnt/β‑catenin signaling feedback loop that serves critical roles in glioma tumorigenesis, and that may represent a potential therapeutic target.

Related Articles

Journal Cover

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Ma Q, Shu C, Xiong J, Li B, Wu J, Zhang S, Li J, Liu J, Wang J, Wang J, et al: MicroRNA‑301a/ZNRF3/wnt/β‑catenin signal regulatory crosstalk mediates glioma progression. Int J Oncol 0: 0-0, 1899
APA
Sun, J., Ma, Q., Shu, C., Xiong, J., Li, B., Wu, J. ... Wang, J. (1899). MicroRNA‑301a/ZNRF3/wnt/β‑catenin signal regulatory crosstalk mediates glioma progression. International Journal of Oncology, 0, 0-0. https://doi.org/10.3892/ijo.2020.5145
MLA
Sun, J., Ma, Q., Shu, C., Xiong, J., Li, B., Wu, J., Zhang, S., Li, J., Liu, J., Wang, J."MicroRNA‑301a/ZNRF3/wnt/β‑catenin signal regulatory crosstalk mediates glioma progression". International Journal of Oncology 0.0 (1899): 0-0.
Chicago
Sun, J., Ma, Q., Shu, C., Xiong, J., Li, B., Wu, J., Zhang, S., Li, J., Liu, J., Wang, J."MicroRNA‑301a/ZNRF3/wnt/β‑catenin signal regulatory crosstalk mediates glioma progression". International Journal of Oncology 0, no. 0 (1899): 0-0. https://doi.org/10.3892/ijo.2020.5145