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Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules

  • Authors:
    • Amina Bolatkan
    • Ken Asada
    • Syuzo Kaneko
    • Kruthi Suvarna
    • Noriko Ikawa
    • Hidenori Machino
    • Masaaki Komatsu
    • Shuichiro Shiina
    • Ryuji Hamamoto
  • View Affiliations / Copyright

    Affiliations: Department of Diagnostic Imaging and Interventional Oncology, Graduate School of Medicine, Juntendo University, Tokyo 113‑8421, Japan, Division of Medical AI Research and Development, National Cancer Center Research Institute, Tokyo 104‑0045, Japan
    Copyright: © Bolatkan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 4
    |
    Published online on: December 14, 2021
       https://doi.org/10.3892/ijo.2021.5294
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Abstract

RNA modifications have attracted increasing interest in recent years because they have been frequently implicated in various human diseases, including cancer, highlighting the importance of dynamic post‑transcriptional modifications. Methyltransferase‑like 6 (METTL6) is a member of the RNA methyltransferase family that has been identified in many cancers; however, little is known about its specific role or mechanism of action. In the present study, we aimed to study the expression levels and functional role of METTL6 in hepatocellular carcinoma (HCC), and further investigate the relevant pathways. To this end, we systematically conducted bioinformatics analysis of METTL6 in HCC using gene expression data and clinical information from a publicly available dataset. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non‑tumor tissues and strongly associated with poorer survival outcomes in patients with HCC. CRISPR/Cas9‑mediated knockout of METTL6 in HCC cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability. RNA sequencing analysis demonstrated that knockout of METTL6 significantly suppressed the expression of cell adhesion‑related genes. However, chromatin immunoprecipitation sequencing results revealed no significant differences in enhancer activities between cells, which suggests that METTL6 may regulate genes of interest post‑transcriptionally. In addition, it was demonstrated for the first time that METTL6 was localized in the cytosol as detected by immunofluorescence analysis, which indicates the plausible location of RNA modification mediated by METTL6. Our findings provide further insight into the function of RNA modifications in cancer and suggest a possible role of METTL6 as a therapeutic target in HCC.
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View References

1 

Boccaletto P, Machnicka MA, Purta E, Piatkowski P, Baginski B, Wirecki TK, de Crécy-Lagard V, Ross R, Limbach PA, Kotter A, et al: MODOMICS: A database of RNA modification pathways. 2017 update. Nucleic Acids Res. 46:D303–D307. 2018. View Article : Google Scholar :

2 

Barbieri I and Kouzarides T: Role of RNA modifications in cancer. Nat Rev Cancer. 20:303–322. 2020. View Article : Google Scholar : PubMed/NCBI

3 

Delaunay S and Frye M: RNA modifications regulating cell fate in cancer. Nat Cell Biol. 21:552–559. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Roundtree IA, Evans ME, Pan T and He C: Dynamic RNA modifications in gene expression regulation. Cell. 169:1187–1200. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Asada K, Bolatkan A, Takasawa K, Komatsu M, Kaneko S and Hamamoto R: Critical Roles of N6-Methyladenosine (m6A) in cancer and virus infection. Biomolecules. 10:10712020. View Article : Google Scholar

6 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Herath NI, Leggett BA and MacDonald GA: Review of genetic and epigenetic alterations in hepatocarcinogenesis. J Gastroenterol Hepatol. 21:15–21. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Nishida N and Goel A: Genetic and epigenetic signatures in human hepatocellular carcinoma: A systematic review. Curr Genomics. 12:130–137. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Hamamoto R, Furukawa Y, Morita M, Iimura Y, Silva FP, Li M, Yagyu R and Nakamura Y: SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells. Nat Cell Biol. 6:731–740. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Yagyu R, Hamamoto R, Furukawa Y, Okabe H, Yamamura T and Nakamura Y: Isolation and characterization of a novel human gene, VANGL1, as a therapeutic target for hepatocellular carcinoma. Int J Oncol. 20:1173–1178. 2002.PubMed/NCBI

11 

Shigekawa Y, Hayami S, Ueno M, Miyamoto A, Suzaki N, Kawai M, Hirono S, Okada KI, Hamamoto R and Yamaue H: Overexpression of KDM5B/JARID1B is associated with poor prognosis in hepatocellular carcinoma. Oncotarget. 9:34320–34335. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Ryu JW, Kim SK, Son MY, Jeon SJ, Oh JH, Lim JH, Cho S, Jung CR, Hamamoto R, Kim DS and Cho HS: Novel prognostic marker PRMT1 regulates cell growth via downregulation of CDKN1A in HCC. Oncotarget. 8:115444–115455. 2017. View Article : Google Scholar

13 

Chen M, Wei L, Law CT, Tsang FH, Shen J, Cheng CL, Tsang LH, Ho DW, Chiu DK, Lee JM, et al: RNA N6-methyladenosine methyltransferase-like 3 promotes liver cancer progression through YTHDF2-dependent posttranscriptional silencing of SOCS2. Hepatology. 67:2254–2270. 2018. View Article : Google Scholar

14 

Barbieri I, Tzelepis K, Pandolfini L, Shi J, Millán-Zambrano G, Robson SC, Aspris D, Migliori V, Bannister AJ, Han N, et al: Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control. Nature. 552:126–131. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Ma JZ, Yang F, Zhou CC, Liu F, Yuan JH, Wang F, Wang TT, Xu QG, Zhou WP and Sun SH: METTL14 suppresses the metastatic potential of hepatocellular carcinoma by modulating N6-methyladenosine-dependent primary MicroRNA processing. Hepatology. 65:529–543. 2017. View Article : Google Scholar

16 

Bao J, Yu Y, Chen J, He Y, Chen X, Ren Z, Xue C, Liu L, Hu Q, Li J, et al: MiR-126 negatively regulates PLK-4 to impact the development of hepatocellular carcinoma via ATR/CHEK1 pathway. Cell Death Dis. 9:10452018. View Article : Google Scholar : PubMed/NCBI

17 

Zhong L, Liao D, Zhang M, Zeng C, Li X, Zhang R, Ma H and Kang T: YTHDF2 suppresses cell proliferation and growth via destabilizing the EGFR mRNA in hepatocellular carcinoma. Cancer Lett. 442:252–261. 2019. View Article : Google Scholar

18 

Gatza ML, Silva GO, Parker JS, Fan C and Perou CM: An integrated genomics approach identifies drivers of proliferation in luminal-subtype human breast cancer. Nat Genet. 46:1051–1059. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Tan XL, Moyer AM, Fridley BL, Schaid DJ, Niu N, Batzler AJ, Jenkins GD, Abo RP, Li L, Cunningham JM, et al: Genetic variation predicting cisplatin cytotoxicity associated with overall survival in lung cancer patients receiving platinum-based chemotherapy. Clin Cancer Res. 17:5801–5811. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Ignatova VV, Kaiser S, Ho JS, Bing X, Stolz P, Tan YX, Lee CL, Gay FP, Lastres PR, Gerlini R, et al: METTL6 is a tRNA m3C methyltransferase that regulates pluripotency and tumor cell growth. Sci Adv. 6:eaaz45512020. View Article : Google Scholar

21 

Dai X, Jiang W, Ma L, Sun J, Yan X, Qian J, Wang Y, Shi Y, Ni S and Yao N: A metabolism-related gene signature for predicting the prognosis and therapeutic responses in patients with hepatocellular carcinoma. Ann Transl Med. 9:5002021. View Article : Google Scholar : PubMed/NCBI

22 

Lopez-Terrada D, Cheung SW, Finegold MJ and Knowles BB: Hep G2 is a hepatoblastoma-derived cell line. Hum Pathol. 40:1512–1515. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Nakabayashi H and Taketa K: HuH-7 cell line established from a highly differentiated human hepatocellular carcinoma. Okayama Igakkai Zasshi (Journal of Okayama Medical Association). 124:231–238. 2012. View Article : Google Scholar

24 

Yamada T, Abei M, Danjoh I, Shirota R, Yamashita T, Hyodo I and Nakamura Y: Identification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of sorafenib. BMC Cancer. 15:2602015. View Article : Google Scholar : PubMed/NCBI

25 

Kim S, Bolatkan A, Kaneko S, Ikawa N, Asada K, Komatsu M, Hayami S, Ojima H, Abe N, Yamaue H and Hamamoto R: Deregulation of the histone lysine-specific demethylase 1 is involved in human hepatocellular carcinoma. Biomolecules. 9:8102019. View Article : Google Scholar

26 

Schneider CA, Rasband WS and Eliceiri KW: NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 9:671–675. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Zhou Y, Zhou B, Pache L, Chang M, Khodabakhshi AH, Tanaseichuk O, Benner C and Chanda SK: Metascape provides a biologist-oriented resource for the analysis of systems-level datasets. Nat Commun. 10:15232019. View Article : Google Scholar : PubMed/NCBI

28 

Ozawa T, Kaneko S, Szulzewsky F, Qiao Z, Takadera M, Narita Y, Kondo T, Holland EC, Hamamoto R and Ichimura K: C11orf95-RELA fusion drives aberrant gene expression through the unique epigenetic regulation for ependymoma formation. Acta Neuropathol Commun. 9:362021. View Article : Google Scholar : PubMed/NCBI

29 

Langmead B and Salzberg SL: Fast gapped-read alignment with Bowtie 2. Nat Methods. 9:357–359. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Robinson JT, Thorvaldsdottir H, Winckler W, Guttman M, Lander ES, Getz G and Mesirov JP: Integrative genomics viewer. Nat Biotechnol. 29:24–26. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Tang Z, Li C, Kang B, Gao G, Li C and Zhang Z: GEPIA: A web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 45:W98–W102. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Anaya J: OncoLnc: Linking TCGA survival data to mRNAs, miRNAs, and lncRNAs. PeerJ Computer Science. 2:e672016. View Article : Google Scholar

33 

Xu L, Liu X, Sheng N, Oo KS, Liang J, Chionh YH, Xu J, Ye F, Gao YG, Dedon PC and Fu XY: Three distinct 3-methylcytidine (m3C) methyltransferases modify tRNA and mRNA in mice and humans. J Biol Chem. 292:14695–14703. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Richon VM, Johnston D, Sneeringer CJ, Jin L, Majer CR, Elliston K, Jerva LF, Scott MP and Copeland RA: Chemogenetic analysis of human protein methyltransferases. Chem Biol Drug Des. 78:199–210. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Leitinger B and Hohenester E: Mammalian collagen receptors. Matrix Biol. 26:146–155. 2007. View Article : Google Scholar

36 

Wilcox ER, Burton QL, Naz S, Riazuddin S, Smith TN, Ploplis B, Belyantseva I, Ben-Yosef T, Liburd NA, Morell RJ, et al: Mutations in the gene encoding tight junction claudin-14 cause autosomal recessive deafness DFNB29. Cell. 104:165–172. 2001. View Article : Google Scholar : PubMed/NCBI

37 

Klar A, Baldassare M and Jessell TM: F-spondin: A gene expressed at high levels in the floor plate encodes a secreted protein that promotes neural cell adhesion and neurite extension. Cell. 69:95–110. 1992. View Article : Google Scholar : PubMed/NCBI

38 

Liu X, Tian H, Li H, Ge C, Zhao F, Yao M and Li J: Derivate Isocorydine (d-ICD) suppresses migration and invasion of hepatocellular carcinoma cell by downregulating ITGA1 expression. Int J Mol Sci. 18:5142017. View Article : Google Scholar :

39 

Dai W, Huang HL, Hu M, Wang SJ, He HJ, Chen NP and Li MY: microRNA-506 regulates proliferation, migration and invasion in hepatocellular carcinoma by targeting F-spondin 1 (SPON1). Am J Cancer Res. 5:2697–2707. 2015.PubMed/NCBI

40 

Li CP, Cai MY, Jiang LJ, Mai SJ, Chen JW, Wang FW, Liao YJ, Chen WH, Jin XH, Pei XQ, et al: CLDN14 is epigenetically silenced by EZH2-mediated H3K27ME3 and is a novel prognostic biomarker in hepatocellular carcinoma. Carcinogenesis. 37:557–566. 2016. View Article : Google Scholar : PubMed/NCBI

41 

Arimbasseri AG, Iben J, Wei FY, Rijal K, Tomizawa K, Hafner M and Maraia RJ: Evolving specificity of tRNA 3-methyl-cytidine-32 (m3C32) modification: A subset of tRNAsSer requires N6-isopentenylation of A37. RNA. 22:1400–1410. 2016. View Article : Google Scholar : PubMed/NCBI

42 

Hopper AK and Huang HY: Quality control pathways for nucleus-encoded eukaryotic tRNA biosynthesis and subcellular trafficking. Mol Cell Biol. 35:2052–2058. 2015. View Article : Google Scholar : PubMed/NCBI

43 

Zhang Z, Ye Y, Gong J, Ruan H, Liu CJ, Xiang Y, Cai C, Guo AY, Ling J and Diao L: Global analysis of tRNA and translation factor expression reveals a dynamic landscape of translational regulation in human cancers. Commun Biol. 1:2342018. View Article : Google Scholar : PubMed/NCBI

44 

Santos M, Fidalgo A, Varanda AS, Oliveira C and Santos MA: tRNA deregulation and its consequences in cancer. Trends Mol Med. 25:853–865. 2019. View Article : Google Scholar : PubMed/NCBI

45 

Huang H, Weng H, Zhou K, Wu T, Zhao BS, Sun M, Chen Z, Deng X, Xiao G, Auer F, et al: Histone H3 trimethylation at lysine 36 guides m6A RNA modification co-transcriptionally. Nature. 567:414–419. 2019. View Article : Google Scholar : PubMed/NCBI

46 

Li Y, Xia L, Tan K, Ye X, Zuo Z, Li M, Xiao R, Wang Z, Liu X, Deng M, et al: N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2. Nat Genet. 52:870–877. 2020. View Article : Google Scholar : PubMed/NCBI

47 

Cheng JX, Chen L, Li Y, Cloe A, Yue M, Wei J, Watanabe KA, Shammo JM, Anastasi J, Shen QJ, et al: RNA cytosine methylation and methyltransferases mediate chromatin organization and 5-azacytidine response and resistance in leukaemia. Nat Commun. 9:11632018. View Article : Google Scholar : PubMed/NCBI

48 

Pereira M, Francisco S, Varanda AS, Santos M, Santos MA and Soares AR: Impact of tRNA Modifications and tRNA-modifying enzymes on proteostasis and human disease. Int J Mol Sci. 19:37382018. View Article : Google Scholar

49 

Hoffer ED, Hong S, Sunita S, Maehigashi T, Gonzalez RL Jnr, Whitford PC and Dunham CM: Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon-anticodon pairing. Elife. 9:e518982020. View Article : Google Scholar : PubMed/NCBI

50 

McLaughlin CS, Grundberg-Manago M, Dondon J, Michelson AM and Saunders G: Stability of the messenger RNA-transfer RNA-ribosome complex. J Mol Biol. 32:521–542. 1968. View Article : Google Scholar : PubMed/NCBI

51 

Alexandrov A, Chernyakov I, Gu W, Hiley SL, Hughes TR, Grayhack EJ and Phizicky EM: Rapid tRNA decay can result from lack of nonessential modifications. Mol Cell. 21:87–96. 2006. View Article : Google Scholar : PubMed/NCBI

52 

Carneiro RL, Requiao RD, Rossetto S, Domitrovic T and Palhano FL: Codon stabilization coefficient as a metric to gain insights into mRNA stability and codon bias and their relationships with translation. Nucleic Acids Res. 47:2216–2228. 2019. View Article : Google Scholar : PubMed/NCBI

53 

Wu Q, Medina SG, Kushawah G, DeVore ML, Castellano LA, Hand JM, Wright M and Bazzini AA: Translation affects mRNA stability in a codon-dependent manner in human cells. Elife. 8:e453962019. View Article : Google Scholar : PubMed/NCBI

54 

Lyu X, Yang Q, Li L, Dang Y, Zhou Z, Chen S and Liu Y: Adaptation of codon usage to tRNA I34 modification controls translation kinetics and proteome landscape. PLoS Genet. 16:e10088362020. View Article : Google Scholar : PubMed/NCBI

55 

Motorin Y and Helm M: tRNA stabilization by modified nucleotides. Biochemistry. 49:4934–4944. 2010. View Article : Google Scholar : PubMed/NCBI

56 

Kimura S and Waldor MK: The RNA degradosome promotes tRNA quality control through clearance of hypomodified tRNA. Proc Natl Acad Sci USA. 116:1394–1403. 2019. View Article : Google Scholar : PubMed/NCBI

57 

Schimmel P: The emerging complexity of the tRNA world: Mammalian tRNAs beyond protein synthesis. Nat Rev Mol Cell Biol. 19:45–58. 2018. View Article : Google Scholar

58 

Xie Y, Yao L, Yu X, Ruan Y, Li Z and Guo J: Action mechanisms and research methods of tRNA-derived small RNAs. Signal Transduct Target Ther. 5:1092020. View Article : Google Scholar : PubMed/NCBI

59 

Lyons SM, Fay MM and Ivanov P: The role of RNA modifications in the regulation of tRNA cleavage. FEBS Lett. 592:2828–2844. 2018. View Article : Google Scholar : PubMed/NCBI

60 

Kim HK, Fuchs G, Wang S, Wei W, Zhang Y, Park H, Roy-Chaudhuri B, Li P, Xu J, Chu K, et al: A transfer-RNA-derived small RNA regulates ribosome biogenesis. Nature. 552:57–62. 2017. View Article : Google Scholar : PubMed/NCBI

61 

Wan J, Wen D, Dong L, Tang J, Liu D, Liu Y, Tao Z, Gao D, Sun H, Cao Y, et al: Establishment of monoclonal HCC cell lines with organ site-specific tropisms. BMC Cancer. 15:6782015. View Article : Google Scholar : PubMed/NCBI

62 

Gharibi A, La Kim S, Molnar J, Brambilla D, Adamian Y, Hoover M, Hong J, Lin J, Wolfenden L and Kelber JA: ITGA1 is a pre-malignant biomarker that promotes therapy resistance and metastatic potential in pancreatic cancer. Sci Rep. 7:100602017. View Article : Google Scholar : PubMed/NCBI

63 

Li H, Wang Y, Rong SK, Li L, Chen T, Fan YY, Wang YF, Yang CR, Yang C, Cho WC and Yang J: Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer. Int J Biol Sci. 16:815–826. 2020. View Article : Google Scholar :

64 

Tryndyak VP, Borowa-Mazgaj B, Steward CR, Beland FA and Pogribny IP: Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells. Arch Toxicol. 94:3993–4005. 2020. View Article : Google Scholar : PubMed/NCBI

65 

Cheng J, Han J and Lin C: A comprehensive assessment of the prognostic role of cell adhesion molecules in acute myeloid leukemia. Transl Cancer Res. 9:7605–7618. 2020. View Article : Google Scholar

66 

Yang G, Jian L and Chen Q: Comprehensive analysis of expression and prognostic value of the claudin family in human breast cancer. Aging (Albany NY). 13:8777–8796. 2021. View Article : Google Scholar

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Bolatkan A, Asada K, Kaneko S, Suvarna K, Ikawa N, Machino H, Komatsu M, Shiina S and Hamamoto R: Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules. Int J Oncol 60: 4, 2022.
APA
Bolatkan, A., Asada, K., Kaneko, S., Suvarna, K., Ikawa, N., Machino, H. ... Hamamoto, R. (2022). Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules. International Journal of Oncology, 60, 4. https://doi.org/10.3892/ijo.2021.5294
MLA
Bolatkan, A., Asada, K., Kaneko, S., Suvarna, K., Ikawa, N., Machino, H., Komatsu, M., Shiina, S., Hamamoto, R."Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules". International Journal of Oncology 60.1 (2022): 4.
Chicago
Bolatkan, A., Asada, K., Kaneko, S., Suvarna, K., Ikawa, N., Machino, H., Komatsu, M., Shiina, S., Hamamoto, R."Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules". International Journal of Oncology 60, no. 1 (2022): 4. https://doi.org/10.3892/ijo.2021.5294
Copy and paste a formatted citation
x
Spandidos Publications style
Bolatkan A, Asada K, Kaneko S, Suvarna K, Ikawa N, Machino H, Komatsu M, Shiina S and Hamamoto R: Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules. Int J Oncol 60: 4, 2022.
APA
Bolatkan, A., Asada, K., Kaneko, S., Suvarna, K., Ikawa, N., Machino, H. ... Hamamoto, R. (2022). Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules. International Journal of Oncology, 60, 4. https://doi.org/10.3892/ijo.2021.5294
MLA
Bolatkan, A., Asada, K., Kaneko, S., Suvarna, K., Ikawa, N., Machino, H., Komatsu, M., Shiina, S., Hamamoto, R."Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules". International Journal of Oncology 60.1 (2022): 4.
Chicago
Bolatkan, A., Asada, K., Kaneko, S., Suvarna, K., Ikawa, N., Machino, H., Komatsu, M., Shiina, S., Hamamoto, R."Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules". International Journal of Oncology 60, no. 1 (2022): 4. https://doi.org/10.3892/ijo.2021.5294
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