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Article

Expression of acidosis‑dependent genes in human cancer nests

  • Authors:
    • Toshihiko Fukamachi
    • Shunsuke Ikeda
    • Hiromi Saito
    • Masatoshi Tagawa
    • Hiroshi Kobayashi
  • View Affiliations / Copyright

    Affiliations: Graduate School of Pharmaceutical Sciences, Chiba University, Chuo‑ku, Chiba 260‑8675, Japan, Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chuo‑ku, Chiba 260‑8717, Japan
  • Pages: 1160-1166
    |
    Published online on: July 11, 2014
       https://doi.org/10.3892/mco.2014.344
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Abstract

Previous studies investigating cancer cells cultured at acidic pH have shown that the expression level of ~700 genes were more than two‑fold higher than those of the cells cultured in alkaline medium at pH 7.5. The aim of the present study was to confirm whether these acidosis‑induced genes are expressed in human cancer tissues. Therefore, 7 genes were selected from our previous study, which encoded interleukin 32 (IL‑32), lysosomal H+ transporting ATPase, V0 subunit d2 (ATP6V0D2), tumor necrosis factor receptor superfamily, member 9 (TNFRSF9), amphiregulin, schwannoma‑derived growth factor (AREG), v‑erb‑b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB3), PRR5‑ARHGAP8 (LOC553158) and dimethylglycine dehydrogenase (DMGDH), and their expression was examined in human clinical specimens from patients with cancer. In addition, the expression of the gene encoding manganese superoxide dismutase (MnSOD) was examined. The specimens from patients with colon, stomach and renal cancer showed increased MnSOD, IL‑32, and TNFRSF9 transcripts compared to those from non‑tumorous regions of the same patients. Notably, an elevated expression of ATP6V0D2 was found in the specimens from patients with stomach cancer, whereas the expression was decreased in those from patients with colon and renal cancer. The expression of LOC553158 was upregulated in colon and stomach cancer specimens. These results indicate that the investigation of gene expression under acidic conditions is useful for the development of novel cancer markers and/or chemotherapeutic targets.
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1 

Vaupel P, Kallinowski F and Okunieff P: Blood flow, oxygen and nutrient supply, and metabolic microenvironment of human tumors. Cancer Res. 49:6449–6465. 1989.PubMed/NCBI

2 

Dellian M, Helmlinger G, Yuan F and Jain RK: Fluorescence ratio imaging of interstitial pH in solid tumours: effect of glucose on spatial and temporal gradients. Br J Cancer. 74:1206–1215. 1996. View Article : Google Scholar : PubMed/NCBI

3 

Warburg O: On the origin of cancer cells. Science. 123:309–314. 1956. View Article : Google Scholar : PubMed/NCBI

4 

Vukovic V and Tannock IF: Influence of low pH on cytotoxicity of paclitaxel, mitoxantrone and topotecan. Br J Cancer. 75:1167–1172. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Sauvant C, Nowak M, Wirth C, et al: Acidosis induces multi-drug resistance in rat prostate cancer cells (AT1) in vitro and in vivo by increasing the activity of the p-glycoprotein via activation of p38. Int J Cancer. 123:2532–2542. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Zucali PA and Giaccone G: Biology and management of malignant pleural mesothelioma. Eur J Cancer. 42:2706–2714. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Catalano A, Rodilossi S, Rippo MR, Caprari P and Procopio A: Induction of stem cell factor/c-Kit/Slug signal transduction in multidrug-resistant malignant mesothelioma cells. J Biol Chem. 279:46706–46714. 2004. View Article : Google Scholar

8 

Fukamachi T, Chiba Y, Wang X, et al: Tumor specific low pH environments enhance the cytotoxicity of lovastatin and cantharidin. Cancer Lett. 297:182–189. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Nielsen SF, Nordestgaard BG and Bojesen SE: Statin use and reduced cancer-related mortality. N Engl J Med. 367:1792–1802. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Brewer TM, Masuda H and Liu DD: Statin use in primary inflammatory breast cancer: a cohort study. Br J Cancer. 109:318–324. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Fukamachi T, Wang X, Mochizuki Y, et al: Acidic environments enhance the inhibitory effect of statins on proliferation of synovial cells. Int Immunopharmacol. 17:148–153. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Fukamachi T, Saito H, Kakegawa T and Kobayashi H: Different proteins are phosphorylated under acidic environments in Jurkat cells. Immunol Lett. 82:155–158. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Hirata S, Fukamachi T, Sakano H, et al: Extracellular acidic environments induce phosphorylation of ZAP-70 in Jurkat T cells. Immunol Lett. 115:105–109. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Lao Q, Fukamachi T, Saito H, Kuge O, Nishijima M and Kobayashi H: Requirement of an IkappaB-beta COOH terminal region protein for acidic-adaptation in CHO cells. J Cell Physiol. 207:238–243. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Fukamachi T, Lao Q, Okamura S, Saito H and Kobayashi H: CTIB (C-Terminus protein of IkappaB-beta): a novel factor required for acidic adaptation. Adv Exp Med Biol. 584:219–228. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Fukamachi T, Ikeda S, Wang X, et al: Gene expressions for signal transduction under acidic conditions. Genes (Basel). 4:65–85. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Tang X, Lucas JE, Chen JL, et al: Functional interaction between responses to lactic acidosis and hypoxia regulates genomic transcriptional outputs. Cancer Res. 72:491–502. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Darnel J, Lodish H and Baltimore D: Molecular Cell Biology. Scientific American Books, Inc; New York, NY, USA: 1986

19 

Malafa M, Margenthaler J, Webb B, Neitzel L and Christophersen M: MnSOD expression is increased in metastatic gastric cancer. J Surg Res. 88:130–134. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Meng X, Wu J, Pan C, et al: Genetic and epigenetic down-regulation of microRNA-212 promotes colorectal tumor metastasis via dysregulation of MnSOD. Gastroenterology. 145:426–436. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Joosten LA, Heinhuis B, Netea MG and Dinarello CA: Novel insights into the biology of interleukin-32. Cell Mol Life Sci. 70:3883–3892. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Ishigami S, Arigami T, Uchikado Y, et al: IL-32 expression is an independent prognostic marker for gastric cancer. Med Oncol. 30:4722013. View Article : Google Scholar : PubMed/NCBI

23 

Zhao S, Zhang H, Xing Y and Natkunam Y: CD137 ligand is expressed in primary and secondary lymphoid follicles and in B-cell lymphomas: diagnostic and therapeutic implications. Am J Surg Pathol. 37:250–258. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Linggi B and Carpenter G: ErbB receptors: new insights on mechanisms and biology. Trends Cell Biol. 16:649–656. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Izycka-Swieszewska E, Wozniak A, Drozynska E, et al: Expression and significance of HER family receptors in neuroblastic tumors. Clin Exp Metastasis. 28:271–282. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Wilzén A, Krona C, Sveinbjörnsson B, et al: ERBB3 is a marker of a ganglioneuroblastoma/ganglioneuroma-like expression profile in neuroblastic tumours. Mol Cancer. 12:702013.PubMed/NCBI

27 

Sithanandam G and Anderson LM: The ERBB3 receptor in cancer and cancer gene therapy. Cancer Gene Ther. 15:413–448. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Guzman MJ, Shao J and Sheng H: Pro-neoplastic effects of amphiregulin in colorectal carcinogenesis. J Gastrointest Cancer. 44:211–221. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Yotsumoto F, Yagi H, Suzuki SO, et al: Validation of HB-EGF and amphiregulin as targets for human cancer therapy. Biochem Biophys Res Commun. 365:555–561. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Song JY, Lee JK, Lee NW, et al: Microarray analysis of normal cervix, carcinoma in situ, and invasive cervical cancer: identification of candidate genes in pathogenesis of invasion in cervical cancer. Int J Gynecol Cancer. 18:1051–1059. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Binzak BA, Wevers RA, Moolenaar SH, et al: Cloning of dimethylglycine dehydrogenase and a new human inborn error of metabolism, dimethylglycine dehydrogenase deficiency. Am J Hum Genet. 68:839–847. 2001. View Article : Google Scholar

32 

Zeidel ML, Silva P and Seifter JL: Intracellular pH regulation and proton transport by rabbit renal medullary collecting duct cells. Role of plasma membrane proton adenosine triphosphatase. J Clin Invest. 77:113–120. 1986. View Article : Google Scholar

33 

Smith AN, Borthwick KJ and Karet FE: Molecular cloning and characterization of novel tissue-specific isoforms of the human vacuolar H+-ATPase C, G and d subunits, and their evaluation in autosomal recessive distal renal tubular acidosis. Gene. 297:169–177. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fukamachi T, Ikeda S, Saito H, Tagawa M and Kobayashi H: Expression of acidosis‑dependent genes in human cancer nests. Mol Clin Oncol 2: 1160-1166, 2014.
APA
Fukamachi, T., Ikeda, S., Saito, H., Tagawa, M., & Kobayashi, H. (2014). Expression of acidosis‑dependent genes in human cancer nests. Molecular and Clinical Oncology, 2, 1160-1166. https://doi.org/10.3892/mco.2014.344
MLA
Fukamachi, T., Ikeda, S., Saito, H., Tagawa, M., Kobayashi, H."Expression of acidosis‑dependent genes in human cancer nests". Molecular and Clinical Oncology 2.6 (2014): 1160-1166.
Chicago
Fukamachi, T., Ikeda, S., Saito, H., Tagawa, M., Kobayashi, H."Expression of acidosis‑dependent genes in human cancer nests". Molecular and Clinical Oncology 2, no. 6 (2014): 1160-1166. https://doi.org/10.3892/mco.2014.344
Copy and paste a formatted citation
x
Spandidos Publications style
Fukamachi T, Ikeda S, Saito H, Tagawa M and Kobayashi H: Expression of acidosis‑dependent genes in human cancer nests. Mol Clin Oncol 2: 1160-1166, 2014.
APA
Fukamachi, T., Ikeda, S., Saito, H., Tagawa, M., & Kobayashi, H. (2014). Expression of acidosis‑dependent genes in human cancer nests. Molecular and Clinical Oncology, 2, 1160-1166. https://doi.org/10.3892/mco.2014.344
MLA
Fukamachi, T., Ikeda, S., Saito, H., Tagawa, M., Kobayashi, H."Expression of acidosis‑dependent genes in human cancer nests". Molecular and Clinical Oncology 2.6 (2014): 1160-1166.
Chicago
Fukamachi, T., Ikeda, S., Saito, H., Tagawa, M., Kobayashi, H."Expression of acidosis‑dependent genes in human cancer nests". Molecular and Clinical Oncology 2, no. 6 (2014): 1160-1166. https://doi.org/10.3892/mco.2014.344
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