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Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells

  • Authors:
    • Javier Vargas‑Villarreal
    • Marlid Cruz‑Ramos
    • Alba Espino‑Ojeda
    • Hugo Gutierrez‑Hermosillo
    • Enrique Díaz De Leon‑Gonzalez
    • Ofelia Monsivais‑Diaz
    • Rebeca Palacios‑Corona
    • Carlos Alejandro Martinez‑Armenta
    • Francisco González‑Salazar
    • Maria Guadalupe Moreno‑Treviño
    • Francisco Javier Guzman‑De La Garza
  • View Affiliations / Copyright

    Affiliations: Centro de Investigación Biomedica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon 64720, Mexico, Translational Oncology Division, Oncohealth Institute, Health Research Institute of The Jiménez Díaz Foundation, Autonomous University of Madrid, Madrid 28030, Spain, Department of Neurology, Tecnologico de Monterrey, Monterrey, Nuevo Leon 64700, Mexico, High Specialty Medical Unit 1, Bajio National Medical Center, Instituto Mexicano del Seguro Social, Leon, Guanajuato 37328, Mexico, High Specialty Medical Unit 21, Hospital of Traumatology and Orthopedics, Instituto Mexicano Del Seguro Social, Monterrey, Nuevo Leon 64000, Mexico, Basic Sciences Department, School of Medicine, University of Monterrey, San Pedro Garza García, Nuevo Leon 66238, Mexico
  • Article Number: 106
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    Published online on: March 20, 2021
       https://doi.org/10.3892/mco.2021.2268
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Abstract

Malignant ascites (MA) and malignant pleural effusion (MPE) are frequently developed in patients with metastatic cancer; however, the biological properties of these fluids have not been clarified. The present study explored the biological role of a low molecular fraction derived from malignant effusions on the activation of peripheral blood mononuclear cells and on the proliferation of breast cancer cells and fibroblast 55x cells. A <10‑kDa fraction from effusions of 41 oncological patients and 34 individuals without cancer was purified, and its potential role in inhibiting nitric oxide (NO) production on lipopolysaccharide (LPS)‑stimulated peripheral blood mononuclear cells was explored, as well as its cytotoxicity on MCF‑7 breast cancer cells and fibroblast 55x cells. A significant decrease in NO production was observed in the <10‑kDa fraction from malignant effusions. In addition, the acellular fraction from MA decreased the viability of breast cancer cells without affecting human fibroblasts. These data support the presence of low molecular weight molecules in malignant samples with a specific role in inhibiting the defense mechanisms of peripheral blood mononuclear cells and decreasing the viability of breast cancer cells in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Vargas‑Villarreal J, Cruz‑Ramos M, Espino‑Ojeda A, Gutierrez‑Hermosillo H, Díaz De Leon‑Gonzalez E, Monsivais‑Diaz O, Palacios‑Corona R, Martinez‑Armenta CA, González‑Salazar F, Moreno‑Treviño MG, Moreno‑Treviño MG, et al: Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells. Mol Clin Oncol 14: 106, 2021.
APA
Vargas‑Villarreal, J., Cruz‑Ramos, M., Espino‑Ojeda, A., Gutierrez‑Hermosillo, H., Díaz De Leon‑Gonzalez, E., Monsivais‑Diaz, O. ... Guzman‑De La Garza, F.J. (2021). Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells. Molecular and Clinical Oncology, 14, 106. https://doi.org/10.3892/mco.2021.2268
MLA
Vargas‑Villarreal, J., Cruz‑Ramos, M., Espino‑Ojeda, A., Gutierrez‑Hermosillo, H., Díaz De Leon‑Gonzalez, E., Monsivais‑Diaz, O., Palacios‑Corona, R., Martinez‑Armenta, C. A., González‑Salazar, F., Moreno‑Treviño, M. G., Guzman‑De La Garza, F. J."Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells". Molecular and Clinical Oncology 14.5 (2021): 106.
Chicago
Vargas‑Villarreal, J., Cruz‑Ramos, M., Espino‑Ojeda, A., Gutierrez‑Hermosillo, H., Díaz De Leon‑Gonzalez, E., Monsivais‑Diaz, O., Palacios‑Corona, R., Martinez‑Armenta, C. A., González‑Salazar, F., Moreno‑Treviño, M. G., Guzman‑De La Garza, F. J."Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells". Molecular and Clinical Oncology 14, no. 5 (2021): 106. https://doi.org/10.3892/mco.2021.2268
Copy and paste a formatted citation
x
Spandidos Publications style
Vargas‑Villarreal J, Cruz‑Ramos M, Espino‑Ojeda A, Gutierrez‑Hermosillo H, Díaz De Leon‑Gonzalez E, Monsivais‑Diaz O, Palacios‑Corona R, Martinez‑Armenta CA, González‑Salazar F, Moreno‑Treviño MG, Moreno‑Treviño MG, et al: Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells. Mol Clin Oncol 14: 106, 2021.
APA
Vargas‑Villarreal, J., Cruz‑Ramos, M., Espino‑Ojeda, A., Gutierrez‑Hermosillo, H., Díaz De Leon‑Gonzalez, E., Monsivais‑Diaz, O. ... Guzman‑De La Garza, F.J. (2021). Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells. Molecular and Clinical Oncology, 14, 106. https://doi.org/10.3892/mco.2021.2268
MLA
Vargas‑Villarreal, J., Cruz‑Ramos, M., Espino‑Ojeda, A., Gutierrez‑Hermosillo, H., Díaz De Leon‑Gonzalez, E., Monsivais‑Diaz, O., Palacios‑Corona, R., Martinez‑Armenta, C. A., González‑Salazar, F., Moreno‑Treviño, M. G., Guzman‑De La Garza, F. J."Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells". Molecular and Clinical Oncology 14.5 (2021): 106.
Chicago
Vargas‑Villarreal, J., Cruz‑Ramos, M., Espino‑Ojeda, A., Gutierrez‑Hermosillo, H., Díaz De Leon‑Gonzalez, E., Monsivais‑Diaz, O., Palacios‑Corona, R., Martinez‑Armenta, C. A., González‑Salazar, F., Moreno‑Treviño, M. G., Guzman‑De La Garza, F. J."Acellular fraction from malignant effusions has cytotoxicity in breast cancer cells". Molecular and Clinical Oncology 14, no. 5 (2021): 106. https://doi.org/10.3892/mco.2021.2268
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