A simple and practical method that prepares high molecular weight DNA ladders

Zhang,Jun-He; Yang,Rui; Wang,Tian-Yun; Dong,Wei-Hua; Wang,Fang; Wang,Li

doi:10.3892/mmr.2012.1061

November 2012 Volume 6 Issue 5

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November 2012 Volume 6 Issue 5

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Article

A simple and practical method that prepares high molecular weight DNA ladders

Authors:
- Jun-He Zhang
- Rui Yang
- Tian-Yun Wang
- Wei-Hua Dong
- Fang Wang
- Li Wang
View Affiliations / Copyright

Affiliations: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Pages: 1211-1213
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Published online on: September 4, 2012

https://doi.org/10.3892/mmr.2012.1061
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Abstract

The purpose of the current study was to report a simple and practical method to prepare high molecular weight (mw) DNA ladders. The method involves 1,000-4,000-base pairs (bp) DNA fragments being amplified by polymerase chain reaction (PCR), using λ DNA as a template. The constructed plasmids are digested by restriction endonucleases to produce 5-, 6-, 8- and 10-kb DNA fragments, followed by purification and precipitation with ethanol, and mixed proportionally. The 1,000-4,000-bp DNA fragments were successfully generated by PCR and 5-, 6-, 8- and 10-kb DNA fragments were obtained through the digestion of the plasmids. The bands of the prepared high mw DNA ladder were clear and may aid future molecular biology studies.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A simple and practical method that prepares high molecular weight DNA ladders

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