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Article

Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells

  • Authors:
    • Jing Tian
    • Yuyang Jiang
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, Tsinghua University, Beijing 100084, P.R. China, The Ministry-Province Jointly Constructed Base for State Key Laboratory, Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P.R. China
  • Pages: 1379-1384
    |
    Published online on: October 1, 2012
       https://doi.org/10.3892/mmr.2012.1113
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Abstract

Zinc finger and BTB domain-containing 7A (Zbtb7A) is a proto-oncogene overexpressed in numerous cancers. In this study, we explored the mechanism of insulin-induced Zbtb7A expression. Real-time PCR and western blotting were used to detect Zbtb7A expression. Zbtb7A promoter activity was monitored by Luciferase reporter assay. It was shown that insulin elevates the mRNA and protein levels of the Zbtb7A gene in HepG2 cells. Using chemical inhibitors of insulin downstream pathways, we demonstrated that the insulin‑induced Zbtb7A gene expression was completely blocked by LY294002, a PI3K/AKT inhibitor, and partially attenuated by the MAPK inhibitor PD98059. Transfection of HepG2 cells with a 1 kb Zbtb7A promoter-luciferase reporter construct revealed a dose‑dependent activation of the Zbtb7A promoter by insulin, while mutation of the Sp1 binding site within the Zbtb7A promoter resulted in the failure of insulin‑induced promoter activation, suggesting that insulin increases Zbtb7A expression through transcriptional regulation mediated by Sp1 in HepG2 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Tian J and Jiang Y: Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells. Mol Med Rep 6: 1379-1384, 2012.
APA
Tian, J., & Jiang, Y. (2012). Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells. Molecular Medicine Reports, 6, 1379-1384. https://doi.org/10.3892/mmr.2012.1113
MLA
Tian, J., Jiang, Y."Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells". Molecular Medicine Reports 6.6 (2012): 1379-1384.
Chicago
Tian, J., Jiang, Y."Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells". Molecular Medicine Reports 6, no. 6 (2012): 1379-1384. https://doi.org/10.3892/mmr.2012.1113
Copy and paste a formatted citation
x
Spandidos Publications style
Tian J and Jiang Y: Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells. Mol Med Rep 6: 1379-1384, 2012.
APA
Tian, J., & Jiang, Y. (2012). Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells. Molecular Medicine Reports, 6, 1379-1384. https://doi.org/10.3892/mmr.2012.1113
MLA
Tian, J., Jiang, Y."Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells". Molecular Medicine Reports 6.6 (2012): 1379-1384.
Chicago
Tian, J., Jiang, Y."Insulin upregulates the expression of zinc finger and BTB domain-containing 7A in HepG2 cells". Molecular Medicine Reports 6, no. 6 (2012): 1379-1384. https://doi.org/10.3892/mmr.2012.1113
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