Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January 2013 Volume 7 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January 2013 Volume 7 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus

  • Authors:
    • Ae-Chin Oh
    • Jin Kyung Lee
    • Ha Na Lee
    • Young Jun Hong
    • Yoon Hwan Chang
    • Seok-Il Hong
    • Dong Ho Kim
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Nowon-gu, Seoul 139-706, Republic of Korea, College of Pharmacy, Seoul National University Graduate School, Gwanak-gu, Seoul 151-742, Republic of Korea, KIRAMS Radiation Biorepository, Korea Institute of Radiological and Medical Sciences, Nowon-gu, Seoul 139-706, Republic of Korea, Department of Pediatrics, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Nowon-gu, Seoul 139-706, Republic of Korea
    Copyright: © Oh et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 11-15
    |
    Published online on: October 9, 2012
       https://doi.org/10.3892/mmr.2012.1121
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for many nosocomial and community-acquired infections, resulting in significant morbidity and mortality. A practical way to limit the spread of MRSA is early detection and proper treatment. However, screening culture for MRSA typically requires 2-3 days. The Xpert MRSA assay (Cepheid, Sunnyvale, CA, USA) is a real-time polymerase chain reaction-based assay developed for screening an MRSA-specific DNA sequence within the staphylococcal cassette chromosome in 2 h. Lower respiratory tract specimens, such as transtracheal aspirates (TTAs) and bronchoalveolar lavage fluid (BALF), are commonly obtained from intubated patients. Therefore, using the lower respiratory tract specimens with the Xpert MRSA assay may be a practical tool for patient care. We performed the Xpert MRSA assay on 108 TTA and 21 BALF specimens from 92 patients and compared the results to those obtained by culture. The two assays showed concordant results in 120 (93.0%) cases and discordant results in 9 (7.0%) cases, which were culture‑negative and Xpert MRSA-positive. Among the discordant cases, 5 patients developed culture-positive samples 2-15 days after the Xpert MRSA detected MRSA. We conclude that the Xpert MRSA assay is a rapid, sensitive and clinically useful test, particularly for the early detection of MRSA.
View Figures
View References

1 

Kim TS, Yi JY, Hong KH, Park JS and Kim EC: Distribution of virulence genes in spa types of methicillin-resistant Staphylococcus aureus isolated from patients in intensive care units. Korean J Lab Med. 31:30–36. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Van Hal SJ, Stark D, Lockwood B, Marriott D and Harkness J: Methicillin-resistant Staphylococcus aureus (MRSA) detection: comparison of 2 molecular methods (IDI-MRSA PCR assay and GenoType MRSA direct PCR assay) with 3 selective MRSA agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for use with infection-control swabs. J Clin Microbiol. 45:2486–2490. 2007.

3 

Hope WW, Morton AP, Looke DF, Schooneveldt JM and Nimmo GR: A PCR method for the identification of Staphylococcus aureus (MRSA) from screening swabs. Pathology. 36:265–268. 2004.PubMed/NCBI

4 

Stratidis J, Bia FJ and Edberg SC: Use of real-time polymerase chain reaction for identification of methicillin-resistant Staphylococcus aureus directly from positive blood culture bottles. Diagn Microbiol Infect Dis. 58:199–202. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Thomas LC, Gidding HF, Ginn AN, Olma T and Iredell J: Development of a real-time Staphylococcus aureus and MRSA (SAM) PCR for routine blood culture. J Microbiol Methods. 68:296–302. 2007.

6 

Ulrich MP, Christensen DR, Coyne SR, et al: Evaluation of the Cepheid GeneXpert system for detecting Bacillus anthracis. J Appl Microbiol. 100:1011–1016. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Park SH, Jang YH, Sung HS, Kim MN, Kim JS and Park YJ: Performance evaluation of BD GeneOhm MRSA PCR assay for detection of nasal colonization of methicillin-resistant Staphylococcus aureus at endemic intensive care unit. Korean J Lab Med. 29:439–447. 2009.(In Korean).

8 

Oberdorfer K, Pohl S, Frey M, Heeg K and Wendt C: Evaluation of a single-locus real-time polymerase chain reaction as a screening test for specific detection of Staphylococcus aureus in ICU patients. Eur J Clin Microbiol Infect Dis. 25:657–663. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Malhotra-Kumar S, Van Heirstraeten L, Lee A, et al: Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 48:4598–4601. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Laurent C, Bogaerts P, Scoevaerdts D, et al: Evaluation of the Xpert MRSA assay for rapid detection of methicillin-resistant Staphylococcus aureus from nares swabs of geriatric hospitalized patients and failure to detect a specific SCCmec type IV variants. Eur J Clin Microbiol Infect Dis. 29:995–1002. 2010.PubMed/NCBI

11 

Paule SM, Hacek DM, Kufner B, et al: Performance of the BN GeneOhm methicillin-resistant Staphylococcus aureus test before and during high-volume clinical use. J Clin Microbiol. 45:2993–2998. 2007.

12 

Japanese Respiratory Society. Diagnosis of hospital-acquired pneumonia and methods of testing for pathogen. Respirology. 14:S10–S22. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Kluytmans J: Control of Staphylococcus aureus (MRSA) and the value of rapid tests. J Hosp Infect. 65:100–104. 2007.

14 

Wolk DM, Picton E, Johnson D, et al: Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in Nares. J Clin Microbiol. 47:758–764. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Roisin S, Laurent C, Nonhoff C, et al: Positive predictive value of the Xpert MRSA assay diagnostic for universal patient screening at hospital admission: influence of the local ecology. Eur J Clin Microbiol Infect Dis. 31:873–880. 2011. View Article : Google Scholar

16 

Rossney AS, Herra CM, Brennan GI, Morgan PM and O’Connell BO: Evaluation of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay using the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens. J Clin Microbiol. 46:3285–3290. 2008.PubMed/NCBI

17 

San ND, Denis O, Gasasira MF, De Mendona, Nonhoff C and Struelens MJ: Controlled evaluation of the IDI-MRSA assay for detection of colonization by methicillin-resistant Staphylococcus aureus in diverse mucocutaneous specimens. J Clin Microbiol. 45:1098–1101. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Kelly PG, Grabsch EA, Howden BP, Gao W and Grayson ML: Comparison of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) PCR assay, BD GeneOhm MRSA assay, and culture for detection of nasal and cutaneous groin colonization by MRSA. J Clin Microbiol. 47:3769–3772. 2009.PubMed/NCBI

19 

Sarikonda KV, Micek ST, Doherty JA, Reichley RM, Warren D and Koller MH: Methicillin-resistant Staphylococcus aureus nasal colonization is a poor predictor of intensive care unit-acquired of methicillin-resistant Staphylococcus aureus infections requiring antibiotic treatment. Crit Care Med. 38:1991–1995. 2010.

20 

Holfelder M, Eigner U, Turnwald AM, Witte W, Weizenegger M and Fahr A: Direct detection of Staphylococcus aureus in clinical specimens by a nucleic acid-based hybridization assay. Clin Microbiol Infect. 12:1163–1167. 2006.

21 

Paule SM, Pasquariello AC, Hacek DM, et al: Direct detection of Staphylococcus aureus from adult and neonate nasal swab specimens using real-time polymerase chain reaction. J Mol Diagn. 6:191–196. 2004.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Oh A, Lee JK, Lee HN, Hong YJ, Chang YH, Hong S and Kim DH: Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus. Mol Med Rep 7: 11-15, 2013.
APA
Oh, A., Lee, J.K., Lee, H.N., Hong, Y.J., Chang, Y.H., Hong, S., & Kim, D.H. (2013). Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus. Molecular Medicine Reports, 7, 11-15. https://doi.org/10.3892/mmr.2012.1121
MLA
Oh, A., Lee, J. K., Lee, H. N., Hong, Y. J., Chang, Y. H., Hong, S., Kim, D. H."Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus". Molecular Medicine Reports 7.1 (2013): 11-15.
Chicago
Oh, A., Lee, J. K., Lee, H. N., Hong, Y. J., Chang, Y. H., Hong, S., Kim, D. H."Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus". Molecular Medicine Reports 7, no. 1 (2013): 11-15. https://doi.org/10.3892/mmr.2012.1121
Copy and paste a formatted citation
x
Spandidos Publications style
Oh A, Lee JK, Lee HN, Hong YJ, Chang YH, Hong S and Kim DH: Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus. Mol Med Rep 7: 11-15, 2013.
APA
Oh, A., Lee, J.K., Lee, H.N., Hong, Y.J., Chang, Y.H., Hong, S., & Kim, D.H. (2013). Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus. Molecular Medicine Reports, 7, 11-15. https://doi.org/10.3892/mmr.2012.1121
MLA
Oh, A., Lee, J. K., Lee, H. N., Hong, Y. J., Chang, Y. H., Hong, S., Kim, D. H."Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus". Molecular Medicine Reports 7.1 (2013): 11-15.
Chicago
Oh, A., Lee, J. K., Lee, H. N., Hong, Y. J., Chang, Y. H., Hong, S., Kim, D. H."Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus". Molecular Medicine Reports 7, no. 1 (2013): 11-15. https://doi.org/10.3892/mmr.2012.1121
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team